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Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22

Tiantian Chen1,2, Lei Li1,2, Dan Liu1, Yubing Tian1,2, Lingli Li1,2, Jianqi Zeng1,2, Awais Rasheed1, Shuanghe Cao1,2, Xianchun Xia1, Zhonghu He1,2,3, Jindong Liu1,2( ), Yong Zhang1,2( )
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/State Key Laboratory of Crop Gene Resources and Breeding, Ministry of Science and Technology/National Wheat Improvement Center, Beijing 100081, China
Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang 453519, China
International Maize and Wheat Improvement Center (CIMMYT), China Office, c/o CAAS, Beijing 100081, China
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Highlights

  • Genotype and environment both contributes to extensive variation of the incidence of black point.

  • QTL mapping identified six stable QTLs and five of them were developed into KASP markers.

  • Pyramiding favorable alleles of identified QTLs can reduce the incidence of black point.

Abstract

Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade. Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat. Here, 262 recombinant inbred lines (RILs) from the cross of Zhongmai 578/Jimai 22 were evaluated for their black point reactions in five environments. A high-density genetic linkage map of the RIL population was constructed with the wheat 50K single nucleotide polymorphism (SNP) array. Six stable QTLs for black point resistance were detected, QBp.caas-2A, QBp.caas-2B1, QBp.caas-2B2, QBp.caas-2D, QBp.caas-3A, and QBp.caas-5B, which explained 2.1–28.8% of the phenotypic variances. The resistance alleles of QBp.caas-2B1 and QBp.caas-2B2 were contributed by Zhongmai 578 while the others were from Jimai 22. QBp.caas-2B2, QBp.caas-2D and QBp.caas-3A overlapped with previously reported loci, whereas QBp.caas-2A, QBp.caas-2B1 and QBp.caas-5B are likely to be new. Five kompetitive allele-specific PCR (KASP) markers, Kasp_2A_BP, Kasp_2B1_BP, Kasp_2B2_BP, Kasp_3A_BP, and Kasp_5B_BP, were validated in a natural population of 165 cultivars. The findings of this study provide useful QTLs and molecular markers for the improvement of black point resistance in wheat through marker-assisted breeding.

Keywords

black pointcandidate genecommon wheatkompetitive allele-specific PCRquantitative trait locus

References

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Journal of Integrative Agriculture (JIA)
Volume 24 Issue 9,
September 2025
Pages 3311-3321
DOI: 10.1016/j.jia.2023.12.039

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Cite this article:
Chen T, Li L, Liu D, et al. Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22. Journal of Integrative Agriculture (JIA), 2025, 24(9): 3311-3321. https://doi.org/10.1016/j.jia.2023.12.039

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Received: 21 September 2023
Revised: 30 October 2023
Accepted: 08 November 2023
Published: 03 January 2024
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