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Review Article | Open Access

Molecular mastery: Harnessing DNA technology for disease prevention

Giri Rajasekhar Dornadulaa,fRamakrishna Chilakalab,fSadak Basha ShaikcPramod Kumar MerigadLikhitha ChinthaaYeshwanth GurugariaKranthi Kumar DaSameena Fatima ShaikeSun Hee Cheongb( )
Department of Pharmacy Practice, Annamacharya College of Pharmacy, Rajampet, Andhra Pradesh 516126, India
Department of Marine Bio-Food Science, Chonnam National University, Yeosu 59626, Republic of Korea
College of Dentistry Department of Bio-clinical Sciences, Kuwait University 13110, Kuwait
Department of Pharmacy Practice, Jawaharlal Nehru Technological University - OTPRI, Ananthapuramu, Andhra Pradesh 515001, India
Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh 517502, India

f These authors contributed equally to this work.

Peer review under the responsibility of Chongqing Medical University.

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Abstract

Advances in DNA technology are revolutionizing the landscape of illness prevention, providing new opportunities to combat hereditary abnormalities and chronic diseases before they manifest. CRISPR-Cas9 gene editing, epigenetic alterations, somatic cell genome editing, and RNA-based therapies are all significant advancements in this field. These techniques enable precise changes to DNA and gene expression, potentially preventing a wide range of illnesses at their genetic origins. The following discussion delves into the transformative potential of these technologies. CRISPR-Cas9 is notable for its precision in fixing defective genes, offering promise for avoiding diseases, such as cystic fibrosis and sickle cell anemia. Epigenetic modifications caused by environmental factors are also opening up new avenues for preventing chronic illnesses and malignancies. Furthermore, somatic cell genome editing is a potential, ethically viable strategy as it targets non-reproductive cells, preventing heritable genetic alterations. Along with these developments, RNA-based therapies are emerging as a new avenue for disease prevention. This paper will explore each of these advancements, their applications, and the ethical and technical challenges involved.

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Cite this article:
Dornadula GR, Chilakala R, Shaik SB, et al. Molecular mastery: Harnessing DNA technology for disease prevention. Genes & Diseases, 2026, 13(4). https://doi.org/10.1016/j.gendis.2025.101976

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Received: 04 December 2024
Revised: 15 August 2025
Accepted: 17 September 2025
Published: 11 December 2025
© 2025 The Authors.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).