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Food Science and Human Wellness 2023, 12(6): 2211-2220 https://doi.org/10.1016/j.fshw.2023.03.041
Research Article | Open Access | Issue | Published: 04 April 2023

Stachyose modulates gut microbiota and alleviates DSS-induced ulcerative colitis in mice

Show Author's Information Chen Wanga,b,c,1,  Junying Baib,c,1,  Botao Wangb,d,  Leilei Yub,c,  Fengwei Tianb,c,e,  Jianxin Zhaob,c,  Hao Zhangb,c,e,f,  Huayi Suoa,  Wei Chenb,c,e,  Qixiao Zhaib,c( )
College of Food Science, Southwest University, Chongqing 400715, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Bloomage Biotechnology Co., Ltd., Jinan 250000, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China
Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, China

1 These authors contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Gut microbiota, Colitis, Metabonomics, Prebiotic, Stachyose
Cite this article:
Wang C, Bai J, Wang B, et al. Stachyose modulates gut microbiota and alleviates DSS-induced ulcerative colitis in mice. Food Science and Human Wellness, 2023, 12(6): 2211-2220. https://doi.org/10.1016/j.fshw.2023.03.041
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Abstract Full text About this article

Stachyose is a prebiotic that traditionally extracted from plants, such as vegetable. It has been demonstrated to alleviate intestinal inflammation by regulating gut microbiota, but the mechanism has been unclear. This research aims to detect the potential mechanism of stachyose in alleviating the severity of ulcerative colitis (UC). The results indicated that the administration of stachyose could recover the body weight, protect against the colonic tissue damage, reduce the pro-inflammatory cytokines levels, and reverse the histological abnormalities in UC mice. Oral stachyose could restore DSS-induced disturbance in intestinal bacteria. Besides, the metabolome result of serum samples showed that stachyose treatment significantly altered serum metabolites against inflammatory responses in colitis mice. Also, a significant correlation can be found between 23 metabolite biomarkers and 18 differential genera. Our results provided a strong foundation for the future study of the protective role of stachyose in maintaining intestinal homeostasis.


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About this article

Stachyose modulates gut microbiota and alleviates DSS-induced ulcerative colitis in mice

Show Author's information Chen Wanga,b,c,1, Junying Baib,c,1, Botao Wangb,d, Leilei Yub,c, Fengwei Tianb,c,e, Jianxin Zhaob,c, Hao Zhangb,c,e,f, Huayi Suoa, Wei Chenb,c,e, Qixiao Zhaib,c( )
College of Food Science, Southwest University, Chongqing 400715, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Bloomage Biotechnology Co., Ltd., Jinan 250000, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China
Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, China

1 These authors contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Stachyose is a prebiotic that traditionally extracted from plants, such as vegetable. It has been demonstrated to alleviate intestinal inflammation by regulating gut microbiota, but the mechanism has been unclear. This research aims to detect the potential mechanism of stachyose in alleviating the severity of ulcerative colitis (UC). The results indicated that the administration of stachyose could recover the body weight, protect against the colonic tissue damage, reduce the pro-inflammatory cytokines levels, and reverse the histological abnormalities in UC mice. Oral stachyose could restore DSS-induced disturbance in intestinal bacteria. Besides, the metabolome result of serum samples showed that stachyose treatment significantly altered serum metabolites against inflammatory responses in colitis mice. Also, a significant correlation can be found between 23 metabolite biomarkers and 18 differential genera. Our results provided a strong foundation for the future study of the protective role of stachyose in maintaining intestinal homeostasis.

Keywords: Gut microbiota, Colitis, Metabonomics, Prebiotic, Stachyose

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Received: 10 May 2022
Revised: 07 June 2022
Accepted: 03 July 2022
Published: 04 April 2023
Issue date: November 2023

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