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Food Science and Human Wellness 2023, 12(6): 2451-2459 https://doi.org/10.1016/j.fshw.2023.03.013
Research Article | Open Access | Issue | Published: 04 April 2023

Effect of ultrasonic modification on the protective activity of Flammulina velutipes polysaccharide to prevent ethanol-induced injury on GES-1 cells

Show Author's Information Jinrong Xiaoa,1,  Xin Chena,1,  Jingsi Zhanga,  Yifan Wanga,  Lei Zhonga,  Qiping Zhana,  Qiuhui Hua,b,  Liyan Zhaoa( )
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China

1 These authors contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Gastric ulcer, Flammulina velutipes polysaccharide, Ultrasonic modification, GES-1 cell, Ethanol-induced injury
Cite this article:
Xiao J, Chen X, Zhang J, et al. Effect of ultrasonic modification on the protective activity of Flammulina velutipes polysaccharide to prevent ethanol-induced injury on GES-1 cells. Food Science and Human Wellness, 2023, 12(6): 2451-2459. https://doi.org/10.1016/j.fshw.2023.03.013
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Abstract Full text About this article

Flammulina velutipes (F. velutipes) polysaccharides were modified by ultrasound at the rated power of 150 W and 900 W. The monosaccharide composition, ultraviolet-visible, and Fourier transform infrared spectral characteristics of F. velutipes polysaccharides (FVP) and their ultrasonic modification products (U-FVPs) were determined. The protective effects of FVP and U-FVPs on human gastric mucosal cells GES-1 were confirmed for the first time. The mole ratios of glucose and galactose were decreased and the mole ratio of mannose was increased after ultrasonic modification. Compared with the original FVP and the FVP modified by ultrasound of 150 W (U-FVP1), the FVP modified by ultrasound of 900 W (U-FVP2) could better prevent ethanol-induced damage to GES-1 cells. With increasing ultrasound intensity, the protective effect of FVPs on GES-1 cells was significantly enhanced by more effective prevention of intracellular reactive oxygen species (ROS) production and more promotion of expression of triglyceride factor 2 (TFF2), prostaglandin E2 (PGE2), epidermal growth factor (EGF), and transforming growth factor β1 (TGF-β1) mRNA. The ultrasonic modification might be an effective way to develop novel F. velutipes polysaccharides that could effectively resist the gastric injury caused by excessive alcohol consumption.


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About this article

Effect of ultrasonic modification on the protective activity of Flammulina velutipes polysaccharide to prevent ethanol-induced injury on GES-1 cells

Show Author's information Jinrong Xiaoa,1, Xin Chena,1, Jingsi Zhanga, Yifan Wanga, Lei Zhonga, Qiping Zhana, Qiuhui Hua,b, Liyan Zhaoa( )
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China

1 These authors contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Flammulina velutipes (F. velutipes) polysaccharides were modified by ultrasound at the rated power of 150 W and 900 W. The monosaccharide composition, ultraviolet-visible, and Fourier transform infrared spectral characteristics of F. velutipes polysaccharides (FVP) and their ultrasonic modification products (U-FVPs) were determined. The protective effects of FVP and U-FVPs on human gastric mucosal cells GES-1 were confirmed for the first time. The mole ratios of glucose and galactose were decreased and the mole ratio of mannose was increased after ultrasonic modification. Compared with the original FVP and the FVP modified by ultrasound of 150 W (U-FVP1), the FVP modified by ultrasound of 900 W (U-FVP2) could better prevent ethanol-induced damage to GES-1 cells. With increasing ultrasound intensity, the protective effect of FVPs on GES-1 cells was significantly enhanced by more effective prevention of intracellular reactive oxygen species (ROS) production and more promotion of expression of triglyceride factor 2 (TFF2), prostaglandin E2 (PGE2), epidermal growth factor (EGF), and transforming growth factor β1 (TGF-β1) mRNA. The ultrasonic modification might be an effective way to develop novel F. velutipes polysaccharides that could effectively resist the gastric injury caused by excessive alcohol consumption.

Keywords: Gastric ulcer, Flammulina velutipes polysaccharide, Ultrasonic modification, GES-1 cell, Ethanol-induced injury

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Received: 15 May 2022
Revised: 05 June 2022
Accepted: 23 June 2022
Published: 04 April 2023
Issue date: November 2023

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© 2023 Beijing Academy of Food Sciences.

Acknowledgements

This research was supported by the Special Funds for Scientific and Technological Achievement Transformation Project in Jiangsu Province (BA2021062).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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