<i>Chimonanthus nitens</i> Oliv. leaves flavonoids alleviate hyperuricemia by regulating uric acid metabolism and intestinal homeostasis in mice

Wenya Meng; Lingli Chen; Kehui Ouyang; Suyun Lin; Yang Zhang; Jing He; Wenjun Wang

doi:10.1016/j.fshw.2023.03.011

Food Science and Human Wellness 2023, 12(6): 2440-2450 https://doi.org/10.1016/j.fshw.2023.03.011

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Open Access | Issue | Published: 04 April 2023

Chimonanthus nitens Oliv. leaves flavonoids alleviate hyperuricemia by regulating uric acid metabolism and intestinal homeostasis in mice

Show Author's Information Hide Author's Information Wenya Meng^{^a}, Lingli Chen^{^a}, Kehui Ouyang^{^b}, Suyun Lin^{^a}, Yang Zhang^{^a}, Jing He^{^a}, Wenjun Wang^{^a}(

)

Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China

College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China

Keywords:

Gut microbiota, Flavonoids, Hyperuricemia, Chimonanthus nitens Oliv. leaves, Renal damage

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Meng W, Chen L, Ouyang K, et al. Chimonanthus nitens Oliv. leaves flavonoids alleviate hyperuricemia by regulating uric acid metabolism and intestinal homeostasis in mice. Food Science and Human Wellness, 2023, 12(6): 2440-2450. https://doi.org/10.1016/j.fshw.2023.03.011

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Abstract

This study aimed to evaluate the alleviating effect of Chimonanthus nitens Oliv. leaves flavonoids (CLF) on hyperuricemia induced by potassium oxonate in mice. The results showed that CLF lowered the serum levels of uric acid (UA), creatinine and blood urea nitrogen, downregulated hepatic mRNA expressions of xanthine oxidase (XO), phosphate ribose pyrophosphate synthetase (PRPS) and adenosine deaminase (ADA) in hyperuricemia mice. In addition, CLF repaired renal injury by significantly down-regulating mRNA and protein expressions of renal UA reabsorption-related proteins and up-regulating the mRNA and protein expressions of UA secretory-related proteins. Finally, CLF inhibited UA synthesis and promoted UA excretion to alleviate hyperuricemia. Besides, CLF supplementation repaired the intestinal barrier function as demonstrated by significant increased mRNA levels of intestinal zonula occludens-1 (ZO-1), Occludin, mucin 2 (MUC2) and mucin 4 (MUC4), as well as decreased mRNA levels of toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88) in mice. Further research showed that CLF treatment restored intestinal homeostasis mediated by improving the composition of gut microbiota and elevating the abundance of beneficial bacteria like Lactobacillus, Alistipes, Prevotellaceae_UCG-001 and Parasutterella. Overall, our findings revealed a novel function of CLF as a promising therapeutic candidate for the treatment of hyperuricemia.

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Chimonanthus nitens Oliv. leaves flavonoids alleviate hyperuricemia by regulating uric acid metabolism and intestinal homeostasis in mice

Show Author's information Hide Author's Information Wenya Meng^{^a}, Lingli Chen^{^a}, Kehui Ouyang^{^b}, Suyun Lin^{^a}, Yang Zhang^{^a}, Jing He^{^a}, Wenjun Wang^{^a}(

)

Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China

College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China

Abstract

Keywords: Gut microbiota, Flavonoids, Hyperuricemia, Chimonanthus nitens Oliv. leaves, Renal damage

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Publication history

Received: 08 April 2022

Revised: 10 May 2022

Accepted: 10 May 2022

Published: 04 April 2023

Issue date: November 2023

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Acknowledgements

The authors gratefully acknowledge the financial supports by National Natural Science Foundation of China (31560459), Major Discipline Academic and Technical Leaders Training Program of Jiangxi Province (20182BCB22003), the Earmarked Fund for Jiangxi Agriculture Research System (JXARS-13) and the Graduate Innovative Special Fund Projects of Jiangxi Province, China (YC2021-S343).

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