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Food Science and Human Wellness 2023, 12(6): 2428-2439 https://doi.org/10.1016/j.fshw.2023.03.010
Research Article | Open Access | Issue | Published: 04 April 2023

Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice

Show Author's Information Pengxiang Xua,b,1,  Shuwei Luoa,b,1,  Jiangfeng Songa,b( ),  Zhuqing Daia,  Dajing Lia,  Cai’e Wub
Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Hydrogel, Intestinal microbiota, Colitis, Lutein, Inflammation regulation
Cite this article:
Xu P, Luo S, Song J, et al. Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice. Food Science and Human Wellness, 2023, 12(6): 2428-2439. https://doi.org/10.1016/j.fshw.2023.03.010
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Abstract Full text About this article

In order to effectively deliver lutein to the inflamed colon and better exert its pharmacological activity, this paper constructed a sodium alginate hydrogel-based delivery system loaded with lutein nanoparticles, evaluated the regulation on the expression and secretion of related inflammatory factors in mice with colitis, and its impact on intestinal microbial environment. The results showed that comparing lutein crystal and its nanoparticle, lutein hydrogel alleviated dextran sodium sulfate (DSS)-induced colitis in mice more effectively by adjusting fecal heme content, colon tissue damage, and inflammatory factor levels. Moreover, lutein hydrogel increased the expression of intestinal tight junction proteins zonula occluden-1 (ZO-1), claudin-1 and occludin to maintain the integrity of the intestinal-barrier, inhibited the nuclear factor-κB (NF-κB) pathway and reduced expression and secretion of inflammatory factors including tumour necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), NOD-like receptors 3 (NLRP3) and interleukin (IL)-1β. In addition, the intestinal microbial environment of mice with colitis was improved by down-regulating the relative abundance of Desulfovibrionaceae and up-regulating the relative abundance of Erysipelotrichaceae and Rikenellaceae. As a slow-release carrier to load lutein nanoparticles, sodium alginate-based hydrogel has potential application prospect.


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About this article

Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice

Show Author's information Pengxiang Xua,b,1, Shuwei Luoa,b,1, Jiangfeng Songa,b( ), Zhuqing Daia, Dajing Lia, Cai’e Wub
Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

In order to effectively deliver lutein to the inflamed colon and better exert its pharmacological activity, this paper constructed a sodium alginate hydrogel-based delivery system loaded with lutein nanoparticles, evaluated the regulation on the expression and secretion of related inflammatory factors in mice with colitis, and its impact on intestinal microbial environment. The results showed that comparing lutein crystal and its nanoparticle, lutein hydrogel alleviated dextran sodium sulfate (DSS)-induced colitis in mice more effectively by adjusting fecal heme content, colon tissue damage, and inflammatory factor levels. Moreover, lutein hydrogel increased the expression of intestinal tight junction proteins zonula occluden-1 (ZO-1), claudin-1 and occludin to maintain the integrity of the intestinal-barrier, inhibited the nuclear factor-κB (NF-κB) pathway and reduced expression and secretion of inflammatory factors including tumour necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), NOD-like receptors 3 (NLRP3) and interleukin (IL)-1β. In addition, the intestinal microbial environment of mice with colitis was improved by down-regulating the relative abundance of Desulfovibrionaceae and up-regulating the relative abundance of Erysipelotrichaceae and Rikenellaceae. As a slow-release carrier to load lutein nanoparticles, sodium alginate-based hydrogel has potential application prospect.

Keywords: Hydrogel, Intestinal microbiota, Colitis, Lutein, Inflammation regulation

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Received: 03 January 2022
Revised: 01 February 2022
Accepted: 28 March 2022
Published: 04 April 2023
Issue date: November 2023

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© 2023 Beijing Academy of Food Sciences.

Acknowledgements

Acknowledgements

This work was supported by the Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu Province (Project No. CX (20)3047).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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