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Food Science and Human Wellness 2023, 12(5): 1526-1537 https://doi.org/10.1016/j.fshw.2023.02.022
Research Article | Open Access | Issue | Published: 21 March 2023

Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier

Show Author's Information Yang Liua,b,  Qing Liua,b,  Chengcheng Zhanga,b,  Jianxin Zhaoa,b,  Hao Zhanga,b,c,  Wei Chena,b,  Qixiao Zhaia,b( )
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China
Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Comparative genomics, Akkermansia muciniphila, Intestinal barrier, Strain specificity, Bacterial surface components
Cite this article:
Liu Y, Liu Q, Zhang C, et al. Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier. Food Science and Human Wellness, 2023, 12(5): 1526-1537. https://doi.org/10.1016/j.fshw.2023.02.022
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Abstract Full text About this article

Akkermansia muciniphila, one of the most promising next-generation probiotics, was reported to exhibit beneficial modulatory effects on the gut barrier. However, the strain-specific and underlying regulatory mechanisms of this species on gut barrier function were not well studied. Therefore, this study evaluated the protective effect of A. muciniphila strains on the intestinal barrier and investigated the mode of action and material basis of this modulatory effect. We first confirmed the strain-specific effects of A. muciniphila on intestinal barrier regulation and found that this phenomenon may be explained by the different abilities of strains to affect tight junction protein expression in enterocytes. Comparative genomic analysis proved that the ability of A. muciniphila to regulate the intestinal barrier was exerted in part by the functional genes (such as COG0438, COG0463, and COG2244) related to the synthesis of cellular surface proteins. The role of these surface proteins in intestinal barrier regulation was further verified by strain-comparative experiments in animal and cell models and surface protein removal trials. This study confirmed the different effects of A. muciniphila strains on gut barrier modulation and provided molecular and genetic targets for the screening of A. muciniphila strains with superior protection against gut barrier dysfunction.


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About this article

Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier

Show Author's information Yang Liua,b, Qing Liua,b, Chengcheng Zhanga,b, Jianxin Zhaoa,b, Hao Zhanga,b,c, Wei Chena,b, Qixiao Zhaia,b( )
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China
Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Akkermansia muciniphila, one of the most promising next-generation probiotics, was reported to exhibit beneficial modulatory effects on the gut barrier. However, the strain-specific and underlying regulatory mechanisms of this species on gut barrier function were not well studied. Therefore, this study evaluated the protective effect of A. muciniphila strains on the intestinal barrier and investigated the mode of action and material basis of this modulatory effect. We first confirmed the strain-specific effects of A. muciniphila on intestinal barrier regulation and found that this phenomenon may be explained by the different abilities of strains to affect tight junction protein expression in enterocytes. Comparative genomic analysis proved that the ability of A. muciniphila to regulate the intestinal barrier was exerted in part by the functional genes (such as COG0438, COG0463, and COG2244) related to the synthesis of cellular surface proteins. The role of these surface proteins in intestinal barrier regulation was further verified by strain-comparative experiments in animal and cell models and surface protein removal trials. This study confirmed the different effects of A. muciniphila strains on gut barrier modulation and provided molecular and genetic targets for the screening of A. muciniphila strains with superior protection against gut barrier dysfunction.

Keywords: Comparative genomics, Akkermansia muciniphila, Intestinal barrier, Strain specificity, Bacterial surface components

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Publication history

Received: 17 July 2021
Revised: 17 August 2021
Accepted: 20 August 2021
Published: 21 March 2023
Issue date: September 2023

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© 2023 Beijing Academy of Food Sciences.

Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (BK20200084); The National Natural Science Foundation of China (32021005 and 31871773); and Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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