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Food Science and Human Wellness 2023, 12(5): 1637-1645 https://doi.org/10.1016/j.fshw.2023.02.017
Research Article | Open Access | Issue | Published: 21 March 2023

Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice

Show Author's Information Zitao Guoa,b,  Dalong Yic,  Bo Hud,  Lingyu Zhua,b,  Ji Zhanga,b,  Yuliang Yanga,b,  Chunyu Liua,b,  Yi Shia,b,  Zhenghua Gua,b,  Yu Xina,b,  Huaigao Liuc,  Liang Zhanga,b( )
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China
Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China
Anhui Guotai Biotechnology Co., Ltd, Xuancheng 242100, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Gut microbiota, Peptides, Short-chain fatty acid, Yak bone collagen hydrolysate, Lachnospiraceae
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Guo Z, Yi D, Hu B, et al. Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice. Food Science and Human Wellness, 2023, 12(5): 1637-1645. https://doi.org/10.1016/j.fshw.2023.02.017
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Abstract Full text About this article

In this study, yak bone collagen hydrolysate (YBCH) was produced by mixed proteases and provided to standard-diet mice at a different dose (low dose (LD), medium dose (MD), and high dose (HD)) to investigate its effects on the composition of gut microbiota and short-chain fatty acids (SCFA) production. It was found that YBCH was mainly composed of small molecular peptides whose molecular weight below 2000 Da. Notably, supplementation with different doses of YBCH could significantly downregulate the ratio of Firmicutes to Bacteroidetes in the fecal microbiota. At the family level, the Lachnospiraceae abundance was significantly reduced in the YBCH gavage groups (mean reduction ratio 41.7%, 35.2%, and 36.4% for LD, MD, and HD group, respectively). The predicted functions of gut microbes in the MD group were significantly increased at "lipid metabolism" and "glycan biosynthesis and metabolism". Moreover, the SCFA production in the YBCH groups was elevated. Especially, the concentration of acetic acid, propionic acid, and butyric acid in the MD group was separately increased 79.7%, 89.2%, and 78.8% than that in the NC group. These results indicated that YBCH might be applied in the development of functional food for intestinal microecological regulation.


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About this article

Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice

Show Author's information Zitao Guoa,b, Dalong Yic, Bo Hud, Lingyu Zhua,b, Ji Zhanga,b, Yuliang Yanga,b, Chunyu Liua,b, Yi Shia,b, Zhenghua Gua,b, Yu Xina,b, Huaigao Liuc, Liang Zhanga,b( )
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China
Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China
Anhui Guotai Biotechnology Co., Ltd, Xuancheng 242100, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

In this study, yak bone collagen hydrolysate (YBCH) was produced by mixed proteases and provided to standard-diet mice at a different dose (low dose (LD), medium dose (MD), and high dose (HD)) to investigate its effects on the composition of gut microbiota and short-chain fatty acids (SCFA) production. It was found that YBCH was mainly composed of small molecular peptides whose molecular weight below 2000 Da. Notably, supplementation with different doses of YBCH could significantly downregulate the ratio of Firmicutes to Bacteroidetes in the fecal microbiota. At the family level, the Lachnospiraceae abundance was significantly reduced in the YBCH gavage groups (mean reduction ratio 41.7%, 35.2%, and 36.4% for LD, MD, and HD group, respectively). The predicted functions of gut microbes in the MD group were significantly increased at "lipid metabolism" and "glycan biosynthesis and metabolism". Moreover, the SCFA production in the YBCH groups was elevated. Especially, the concentration of acetic acid, propionic acid, and butyric acid in the MD group was separately increased 79.7%, 89.2%, and 78.8% than that in the NC group. These results indicated that YBCH might be applied in the development of functional food for intestinal microecological regulation.

Keywords: Gut microbiota, Peptides, Short-chain fatty acid, Yak bone collagen hydrolysate, Lachnospiraceae

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Publication history
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Publication history

Received: 12 April 2021
Revised: 07 July 2021
Accepted: 01 August 2021
Published: 21 March 2023
Issue date: September 2023

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© 2023 Beijing Academy of Food Sciences.

Acknowledgements

Acknowledgements

The authors are very grateful for the technical support from the staff of the National Engineering Research Center for Functional Food, Jiangnan University. This work was supported by the Postdoctoral Research Funding of Jiangsu Province (2021K269B) and National Key Research & Developmental Program of China (2018YFA0900300).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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