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Food Science and Human Wellness 2023, 12(4): 1136-1148 https://doi.org/10.1016/j.fshw.2022.10.039
Research Article | Open Access | Issue | Published: 18 November 2022

The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics

Show Author's Information Jun Wanga,b,1,  Xiuxiu Zhangb,1,  Zhijing Nic,  Elnur Elamc,  Kiran Thakurb,c,  Kexin Lia,  Chuyan Wanga,  Jianguo Zhangb,c( ),  Zhaojun Weib,c( )
School of Biological Food and Environment, Hefei University, Hefei 230601, China
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China
School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan 750021, China

1 Co-fi rst author.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:
HepG2 cells, Licochalcone A, Dysregulated miRNAs, Transcription factors, Targets, Regulatory networks
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Wang J, Zhang X, Ni Z, et al. The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics. Food Science and Human Wellness, 2023, 12(4): 1136-1148. https://doi.org/10.1016/j.fshw.2022.10.039
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Abstract Full text About this article

To explore the function of licochalcone A as an anticancer phytochemical on HepG2 cells and investigate its potential mechanisms, we analyzed the microRNAs (miRNAs) expression profile of HepG2 cells in response to licochalcone A (70 μmol/L) in vitro. 102 dysregulated miRNAs were detected, and SP1 was expected as the transcription factor that regulates the functions of most screened miRNAs. A sum of 431 targets, the overlap of predicted mRNAs from TargetScan, miRDB, and miRtarbase were detected as the targets for these dysregulated miRNAs. FoxO signaling pathway was the hub pathway for the targets. A protein-protein interaction network was structured on the STRING platform to discover the hub genes. Among them, PIK3R1, CDC42, ESR1, SMAD4, SUMO1, KRAS, AGO1, etc. were screened out. Afterwards, the miRNA-target networks were established to screen key dysregulated miRNAs. Two key miRNAs (hsa-miR-133b and hsa-miR-145-5p) were filtered. Finally, the miRNA-target-transcription factor networks were constructed for these key miRNAs. The networks for these key miRNAs included three and two transcription factors, respectively. These identified miRNAs, transcription factors, targets, and regulatory networks may offer hints to understand the molecular mechanism of licochalcone A as a natural anticarcinogen.


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About this article

The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics

Show Author's information Jun Wanga,b,1, Xiuxiu Zhangb,1, Zhijing Nic, Elnur Elamc, Kiran Thakurb,c, Kexin Lia, Chuyan Wanga, Jianguo Zhangb,c( ), Zhaojun Weib,c( )
School of Biological Food and Environment, Hefei University, Hefei 230601, China
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China
School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan 750021, China

1 Co-fi rst author.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

To explore the function of licochalcone A as an anticancer phytochemical on HepG2 cells and investigate its potential mechanisms, we analyzed the microRNAs (miRNAs) expression profile of HepG2 cells in response to licochalcone A (70 μmol/L) in vitro. 102 dysregulated miRNAs were detected, and SP1 was expected as the transcription factor that regulates the functions of most screened miRNAs. A sum of 431 targets, the overlap of predicted mRNAs from TargetScan, miRDB, and miRtarbase were detected as the targets for these dysregulated miRNAs. FoxO signaling pathway was the hub pathway for the targets. A protein-protein interaction network was structured on the STRING platform to discover the hub genes. Among them, PIK3R1, CDC42, ESR1, SMAD4, SUMO1, KRAS, AGO1, etc. were screened out. Afterwards, the miRNA-target networks were established to screen key dysregulated miRNAs. Two key miRNAs (hsa-miR-133b and hsa-miR-145-5p) were filtered. Finally, the miRNA-target-transcription factor networks were constructed for these key miRNAs. The networks for these key miRNAs included three and two transcription factors, respectively. These identified miRNAs, transcription factors, targets, and regulatory networks may offer hints to understand the molecular mechanism of licochalcone A as a natural anticarcinogen.

Keywords: HepG2 cells, Licochalcone A, Dysregulated miRNAs, Transcription factors, Targets, Regulatory networks

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Received: 21 March 2021
Revised: 27 March 2021
Accepted: 31 March 2021
Published: 18 November 2022
Issue date: July 2023

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© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

This study was supported by the Hefei University Scientific Research and Development Fund (20ZR09ZDB), the talent fund of Hefei University (20RC48), the University Natural Sciences Research Project of Anhui Province (KJ2021A1009), the Major Projects of Science and Technology in Anhui Province (201903a06020021, 202004a06020042, 202004a06020052, 201904a06020008), and the National Natural Science Foundation of China (31850410476).

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