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Food Science and Human Wellness 2023, 12(4): 1359-1368 https://doi.org/10.1016/j.fshw.2022.10.037
Research Article | Open Access | Issue | Published: 18 November 2022

Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B

Show Author's Information Yangliu Liua,  Yawei Ningb,  Zhou Chena,  Panpan Hana,  Tongxin Zhia,  Siting Lia,  Aijin Maa( ),  Yingmin Jiaa( )
School of Food and Health, Beijing Technology and Business University, Beijing 100048, China
College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Transcriptomics, Antimicrobial lipopeptide, Brevilaterin B, Antibacterial mechanism, Listeria monocytogenes
Cite this article:
Liu Y, Ning Y, Chen Z, et al. Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B. Food Science and Human Wellness, 2023, 12(4): 1359-1368. https://doi.org/10.1016/j.fshw.2022.10.037
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Abstract Full text About this article

Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety. Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L. monocytogenes by interacting with the cell membrane. This research further explored the antibacterial mechanism of brevilaterin B against L. monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis. Brevilaterin B induced growth inhibition rather than direct membrane lysis in L. monocytogenes at the minimum inhibitory concentration. Transcriptomic analysis showed 1779 difference expressed genes, including 895 up-regulated and 884 down-regulated genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L. monocytogenes, including peptidoglycan biosynthesis, membrane transport (ATP-binding cassette transports, ion transport), cellular metabolism (amino acid and lipid metabolism), ATP synthesis, and activation of the stress response (quorum sensing and bacterial chemotaxis). In conclusion, brevilaterin B affects gene expression related to biosynthesis, transport and stress response pathways on the membrane of L. monocytogenes. The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.


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Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B

Show Author's information Yangliu Liua, Yawei Ningb, Zhou Chena, Panpan Hana, Tongxin Zhia, Siting Lia, Aijin Maa( ), Yingmin Jiaa( )
School of Food and Health, Beijing Technology and Business University, Beijing 100048, China
College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety. Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L. monocytogenes by interacting with the cell membrane. This research further explored the antibacterial mechanism of brevilaterin B against L. monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis. Brevilaterin B induced growth inhibition rather than direct membrane lysis in L. monocytogenes at the minimum inhibitory concentration. Transcriptomic analysis showed 1779 difference expressed genes, including 895 up-regulated and 884 down-regulated genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L. monocytogenes, including peptidoglycan biosynthesis, membrane transport (ATP-binding cassette transports, ion transport), cellular metabolism (amino acid and lipid metabolism), ATP synthesis, and activation of the stress response (quorum sensing and bacterial chemotaxis). In conclusion, brevilaterin B affects gene expression related to biosynthesis, transport and stress response pathways on the membrane of L. monocytogenes. The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.

Keywords: Transcriptomics, Antimicrobial lipopeptide, Brevilaterin B, Antibacterial mechanism, Listeria monocytogenes

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Publication history

Received: 07 September 2021
Revised: 27 September 2021
Accepted: 11 October 2021
Published: 18 November 2022
Issue date: July 2023

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© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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This research was financially supported by the National Natural Science Foundation of China (31771951, 32072199, 31801510); the Beijing Natural Science Foundation (KZ201810011016).

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