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Food Science and Human Wellness 2023, 12(4): 929-941 https://doi.org/10.1016/j.fshw.2022.10.016
Review Article | Open Access | Issue | Published: 18 November 2022

Emerging natural hemp seed proteins and their functions for nutraceutical applications

Show Author's Information Haihong Chena,b,  Bing Xua,b,  Yi Wangc( ),  Wei Lia,  Dong Hed,  Yan Zhange,  Xizhen Zhangf,  Xinhui Xinga,b,c,g( )
Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China
Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 440300, China
Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
School of Chemical Engineering and Energy Technology, Engineering Research Center of Health Food Design & Nutrition Regulation, Key Laboratory of Healthy Food Development and Nutrition Regulation of China National Light Industry, Dongguan University of Technology, Dongguan 523808, China
Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China
College of life Science and Technology, Guangxi University, Nanning 530004, China
Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Keywords:
Physicochemical properties, Purification, Extraction, Hemp seed protein, Nutraceutical functions, Food industry
Cite this article:
Chen H, Xu B, Wang Y, et al. Emerging natural hemp seed proteins and their functions for nutraceutical applications. Food Science and Human Wellness, 2023, 12(4): 929-941. https://doi.org/10.1016/j.fshw.2022.10.016
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Abstract Full text About this article

With changing dietary habits and increasing awareness of the nutraceutical role of dietary foods, the demand for natural plant proteins and interest in non-traditional protein sources in the food industry are increasing. Industrial hemp, belonging to the plant family Cannabaceae, is cultivated for its fibre and edible seeds. Due to its nutritional value, it has also been used in the food industry and medicine. In particular, hemp seed proteins have drawn considerable attention in both scientific and industrial fields because of their excellent nutraceutical values, superior digestibility, low allergenicity and diverse techno-functional properties. In this review, we provide a summary of the current research progress on the extraction and purification processes, physiochemical properties, nutraceutical functions, and applications of hemp seed proteins. Perspectives in the application of advanced technologies for hemp seed bioactive peptide mining are also discussed. This review provides up-to-date insights into the nutraceutical values, health benefits, and future applications of this emerging plant source protein.


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About this article

Emerging natural hemp seed proteins and their functions for nutraceutical applications

Show Author's information Haihong Chena,b, Bing Xua,b, Yi Wangc( ), Wei Lia, Dong Hed, Yan Zhange, Xizhen Zhangf, Xinhui Xinga,b,c,g( )
Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China
Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 440300, China
Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
School of Chemical Engineering and Energy Technology, Engineering Research Center of Health Food Design & Nutrition Regulation, Key Laboratory of Healthy Food Development and Nutrition Regulation of China National Light Industry, Dongguan University of Technology, Dongguan 523808, China
Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China
College of life Science and Technology, Guangxi University, Nanning 530004, China
Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China

Abstract

With changing dietary habits and increasing awareness of the nutraceutical role of dietary foods, the demand for natural plant proteins and interest in non-traditional protein sources in the food industry are increasing. Industrial hemp, belonging to the plant family Cannabaceae, is cultivated for its fibre and edible seeds. Due to its nutritional value, it has also been used in the food industry and medicine. In particular, hemp seed proteins have drawn considerable attention in both scientific and industrial fields because of their excellent nutraceutical values, superior digestibility, low allergenicity and diverse techno-functional properties. In this review, we provide a summary of the current research progress on the extraction and purification processes, physiochemical properties, nutraceutical functions, and applications of hemp seed proteins. Perspectives in the application of advanced technologies for hemp seed bioactive peptide mining are also discussed. This review provides up-to-date insights into the nutraceutical values, health benefits, and future applications of this emerging plant source protein.

Keywords: Physicochemical properties, Purification, Extraction, Hemp seed protein, Nutraceutical functions, Food industry

References(67)

[1]

C.E. Turner, M.A. Elsohly, E.G. Boeren, Constituents of C. sativa L. XVⅡ. A review of the natural constituents, J. Nat. Prod. 43 (1980) 169-234.
DOI Google Scholar
[2]
R.E. Aluko, Hemp seed (Cannabis sativa L.) proteins, Canada: Sustainable Protn Sources, 2017.
DOI
[3]

M. Möller, C. Popescu, Chapter 9.6 Natural Fibres. In Sustainable Solutions for Modern Economies. The Royal Society of Chemistry, 2009, pp. 368-393
DOI Google Scholar
[4]
NHS, Chinese medicine and Food homology catalogue, 2021 edition. China: National Health Commission of the People's Republic of China, 2021.
[5]

B. Farinon, R. Molinari, L. Costantini, et al., The seed of industrial hemp (Cannabis sativa L.): nutritional quality and potential functionality for human health and nutrition, Nutrients 12 (2020) 1935. https://doi.org/10.3390/nu12071935.
DOI Google Scholar
[6]

J.L. Deferne, D.W. Pate, Hemp seed oil: a source of valuable essential fatty acids, J. Int. Hemp Assoc. 3 (1996) 4-7.
Google Scholar
[7]

J.D. House, J. Neufeld, G. Leson, Evaluating the quality of protein from hemp seed (Cannabis sativa L.) products through the use of the protein digestibility-corrected amino acid score method, J. Agric. Food Chem. 58(2010) 11801-11807. https://doi.org/10.1021/jf102636b.
DOI Google Scholar
[8]

G. Wu, C.J. Meininger, Regulation of nitric oxide synthesis by dietary factors, Annu. Rev. Nutr. 22 (2002) 61-86. https://doi.org/10.1146/annurev.nutr.22.110901.145329.
DOI Google Scholar
[9]

T. Dapcevic-Hadnadev, M. Dizdar, M. Pojic, et al., Emulsifying properties of hemp proteins: effect of isolation technique, Food Hydrocoll. 89 (2019)912-920. https://doi.org/10.1016/j.foodhyd.2018.12.002.
DOI Google Scholar
[10]

S.A. Malomo, R.E. Aluko, A comparative study of the structural and functional properties of isolated hemp seed (Cannabis sativa L.)albumin and globulin fractions, Food Hydrocoll. 43 (2015) 743-752. https://doi.org/10.1016/j.foodhyd.2014.08.001.
DOI Google Scholar
[11]

S.A. Malomo, H. Rong, R.E. Aluko, Structural and functional properties of hemp seed protein products, J. Food Sci. 79 (2014) C1512-C1521. https://doi.org/10.1111/1750-3841.12537.
DOI Google Scholar
[12]

S.A. Malomo, R.E. Aluko, Conversion of a low protein hemp seed meal into a functional protein concentrate through enzymatic digestion of fibre coupled with membrane ultrafiltration, Innov. Food Sci. Emerg. Technol. 31 (2015)151-159. https://doi.org/10.1016/j.ifset.2015.08.004.
DOI Google Scholar
[13]

M. Hadnadev, T. Dapcevic-Hadnadev, A. Lazaridou, et al., Hempseed meal protein isolates prepared by different isolation techniques. Part Ⅰ. physicochemical properties, Food Hydrocoll. 79 (2018) 526-533. https://doi.org/10.1016/j.foodhyd.2017.12.015.
DOI Google Scholar
[14]

A. Carne, T. Ss, B. Ae-Da, et al., Effect of defatting process, acid and alkali extraction on the physicochemical and functional properties of hemp, flax and canola seed cake protein isolates, Food Measure. 8 (2014) 92-104. https://doi.org/10.1007/s11694-013-9168-x.
DOI Google Scholar
[15]

S. Patel, R. Cudney, A. McPherson, Crystallographic characterization and molecular symmetry of edestin, a legumin from hemp, J. Mol. Biol. 235(1994) 361-363. https://doi.org/10.1016/S0022-2836(05)80040-3.
DOI Google Scholar
[16]

X.S. Wang, C.H. Tang, X.Q. Yang, et al., Characterization, amino acid composition and in vitro digestibility of hemp (Cannabis sativa L.) proteins, Food Chem. 107 (2008) 11-18. https://doi.org/10.1016/j.foodchem.2007.06.064.
DOI Google Scholar
[17]

J.J. Kim, M.Y. Lee, Isolation and characterization of edestin from Cheungsam hempseed, J. Appl. Biol. Chem. 54 (2011) 84-88. https://doi.org/10.3839/jabc.2011.015.
DOI Google Scholar
[18]

S. Odani, S. Odani, Isolation and primary structure of a methionine- and cystine-rich seed protein of Cannabis sativa, Biosci. Biotechnol. Biochem. 62 (1998) 650-654. https://doi.org/10.1271/bbb.62.650.
DOI Google Scholar
[19]

E. Ponzoni, I.M. Brambilla, I. Galasso, Genome-wide identification and organization of seed storage protein genes of Cannabis sativa, Biol. Plant. 62(2018) 1-10. https://doi.org/10.1007/s10535-018-0810-7.
DOI Google Scholar
[20]

J.C. Callaway, Hempseed as a nutritional resource: an overview, Euphytica 140 (2004) 65-72. https://doi.org/10.1007/s10681-004-4811-6.
DOI Google Scholar
[21]

R.R. Lu, R. Qian, R. Sun, et al., Hempseed protein derived antioxidative peptides: purification, identification and protection from hydrogen peroxideinduced apoptosis in PC12 cells, Food Chem. 123 (2010) 1210-1218. https://doi.org/10.1016/j.foodchem.2010.05.089.
DOI Google Scholar
[22]

R. Roberto, R. Remo, Variability in antinutritional compounds in hempseed meal of Italian and French varieties, Plant 1 (2014) 25-29.
DOI Google Scholar
[23]

G. Mamone, G. Picariello, A. Ramondo, et al., Production, digestibility and allergenicity of hemp (Cannabis sativa L.) protein isolates, Food Res. Int. 115 (2019) 562-571. https://doi.org/10.1016/j.foodres.2018.09.017.
DOI Google Scholar
[24]
C.F. Ajibola, Structural and functional properties of hemp seed storage proteins. University of Manitoba, Department of Food and Human Nutritional Sciences; 2020.
[25]

C.H. Tang, T. Zi, X.S. Wang, et al., Physicochemical and functional properties of hemp (Cannabis sativa L.) protein isolate, J. Agric. Food Chem. 54 (2006) 8945-8950. https://doi.org/10.1021/jf0619176.
DOI Google Scholar
[26]

S.W. Yin, C.H. Tang, Q.B. Wen, et al., Properties of cast films from hemp(Cannabis sativa L.) and soy protein isolates. A comparative study, J. Agric. Food Chem. 55 (2007) 7399-7404. https://doi.org/10.1021/jf071117a.
DOI Google Scholar
[27]

D. Goring, P. Johnson, The preparation and stability of ultracentrifugally monodisperse edestin, Arch. Biochem. Biophys. 56 (1955) 448-458. https://doi.org/10.1016/0003-9861(55)90265-4.
DOI Google Scholar
[28]

J. Jiang, Y.L. Xiong, J. Chen, pH shifting alters solubility characteristics and thermal stability of soy protein isolate and its globulin fractions in different pH, salt concentration, and temperature conditions, J. Agric. Food Chem. 58(2010) 8035-8042. https://doi.org/10.1021/jf101045b.
DOI Google Scholar
[29]

Q. Wang, Y.L. Xiong, Processing, nutrition, and functionality of hempseed protein: a review, Compr. Rev. Food Sci. F. 18 (2019) 936-952. https://doi.org/10.1111/1541-4337.12450.
DOI Google Scholar
[30]

A. Moure, J. Sineiro, H. Dominguez, et al., Functionality of oilseed protein products: a review, Food Res. Int. 39 (2006) 945-963. https://doi.org/10.1016/j.foodres.2006.07.002.
DOI Google Scholar
[31]

O. Radočaj, E. Dimić, R. Tsao, Effects of hemp (Cannabis sativa L.) seed oil press-cake and decaffeinated green tea leaves (Camellia sinensis) on functional characteristics of gluten-free crackers, J. Food Sci. 79 (2014)C318-C325. https://doi.org/10.1111/1750-3841.12370.
DOI Google Scholar
[32]

L.E. Abugoch, N. Romero, C.A. Tapia, et al., Study of some physicochemical and functional properties of quinoa (Chenopodium quinoa willd) protein isolates, J. Agric. Food Chem. 56 (2008) 4745. https://doi.org/10.1021/jf703689u.
DOI Google Scholar
[33]
G. Isinguzo: Physicochemical, functional and in vitro bioactive properties of hempseed (Cannabis sativa) protein isolates and hydrolysates. University of Manitoba, Department of Human Nutritional Sciences; 2011.
[34]

S. Damodaran, Structure-function relationship of food proteins, Prot. Funct. Food Syst. 9 (1994) 1-37.
Google Scholar
[35]

J.E. Kinsella, Functional properties of proteins: possible relationships between structure and function in foams, Food Chem. 7 (1981) 273-288. https://doi.org/10.1016/0308-8146(81)90033-9.
DOI Google Scholar
[36]

P. Walstra, Food emulsions: principles, practice and techniques, Trends Food Sci. Tech. 10 (1999) 241. https://doi.org/10.1046/j.1365-2621.2001.00459.x.
DOI Google Scholar
[37]

J. Boye, F. Zare, A. Pletch, Pulse proteins: processing, characterization, functional properties and applications in food and feed, Food Res. Int. 43(2010) 414-431. https://doi.org/10.1016/j.foodres.2009.09.003.
DOI Google Scholar
[38]

L. Amagliani, J. Regan, A.L. Kelly, et al., The composition, extraction, functionality and applications of rice proteins: a review, Trends Food Sci. Tech. 64 (2017) 1-12. https://doi.org/10.1016/j.tifs.2017.01.008.
DOI Google Scholar
[39]
J.M. Krochta, Proteins as raw materials for films and coatings: definitions, current status, and opportunities, Gennadios A. ed. : Protein-Based Films and Coatings, 2002.
DOI
[40]

Y. Wang, Y.L. Xiong, G.K. Rentfrow, et al., Oxidation promotes crosslinking but impairs film-forming properties of whey proteins, J. Food Eng. 115 (2013) 11-19. https://doi.org/10.1016/j.jfoodeng.2012.09.013.
DOI Google Scholar
[41]

L. Mirmoghtadaie, S.S. Aliabadi, S.M. Hosseini, Recent approaches in physical modification of protein functionality, Food Chem. 199 (2016) 619-627. https://doi.org/10.1016/j.foodchem.2015.12.067.
DOI Google Scholar
[42]

Y.L. Li, X.R. Dai, X. Yue, et al., Identification of small secreted peptides(SSPs) in maize and expression analysis of partial SSP genes in reproductive tissues, Planta 240 (2014) 713-728. https://doi.org/10.1007/s00425-014-2123-1.
DOI Google Scholar
[43]

C.H. Tang, X.S. Wang, X.Q. Yang, Enzymatic hydrolysis of hemp(Cannabis sativa L.) protein isolate by various proteases and antioxidant properties of the resulting hydrolysates, Food Chem. 114 (2009) 1484-1490. https://doi.org/10.1016/j.foodchem.2008.11.049.
DOI Google Scholar
[44]

J.Y. Lin, Q.Z. Zeng, A.N. Qi, Research on the liquid fermentation of hemp seed meal by Bacillus subtilis, China Condiment 36 (2011) 31-36.
Google Scholar
[45]
F. Liu, W. Liang, Comparative study on anti-fatigue and immunomodulatory functions of hemp seed protein. In; 2020. WILEY 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, (2020) 290-290.
[46]

S.A. Malomo, R.E. Aluko, In vitro acetylcholinesterase-inhibitory properties of enzymatic hemp seed protein hydrolysates, J. Am. Oil Chem. Soc. 93(2016) 411-420. https://doi.org/10.1007/s11746-015-2779-0.
DOI Google Scholar
[47]

A. Nk, A. Sd, B. Pk, Ameliorative effects of hempseed (Cannabis sativa) against hypercholesterolemia associated cardiovascular changes, Nutr. Metab. Cardiovas. 30 (2020) 330-338. https://doi.org/10.1016/j.numecd.2019.09.006.
DOI Google Scholar
[48]

F. Rivero-Pino, F.J. Espejo-Carpio, E.M. Guadix, Production and identification of dipeptidyl peptidase Ⅳ (DPP-Ⅳ) inhibitory peptides from discarded Sardine pilchardus protein, Food Chem. 328 (2020) 127096.https://doi.org/10.1016/j.foodchem.2020.127096.
DOI Google Scholar
[49]

Y. Xu, J. Zhao, R. Hu, et al., Effect of genotype on the physicochemical, nutritional, and antioxidant properties of hempseed, J. Agric. Food Res. 3(2021) 100119. https://doi.org/10.1016/j.jafr.2021.100119.
DOI Google Scholar
[50]

A.T. Girgih, C.C. Udenigwe, H. Li, et al., Kinetics of enzyme inhibition and antihypertensive effects of hemp seed (Cannabis sativa L.)protein hydrolysates, J. Am. Oil Chem. Soc. 88 (2011) 1767-1774.https://doi.org/10.1007/s11746-011-1841-9.
DOI Google Scholar
[51]

A.T. Girgih, C.C. Udenigwe, R.E. Aluko, Reverse-phase HPLC separation of hemp seed (Cannabis sativa L.) protein hydrolysate produced peptide fractions with enhanced antioxidant capacity, Plant Foods Hum. Nutr. 68(2013) 39-46. https://doi.org/10.1007/s11130-013-0340-6.
DOI Google Scholar
[52]

R.E. Aluko, Antihypertensive peptides from food proteins, Annu.Rev. Food Sci. T. 6 (2015) 235-262. https://doi.org/10.1146/annurevfood-022814-015520.
DOI Google Scholar
[53]

M. Sunday, O. John, G. Abraham, et al., Structural and antihypertensive properties of enzymatic hemp seed protein hydrolysates, Nutrients 7 (2015)7616-7632. https://doi.org/10.3390/nu7095358.
DOI Google Scholar
[54]

H.M. Aukema, J. Gauthier, M. Roy, et al., Distinctive effects of plant protein sources on renal disease progression and associated cardiac hypertrophy in experimental kidney disease, Mol. Nutr. Food Res. 55 (2011) 1044-1051.https://doi.org/10.1002/mnfr.201000558.
DOI Google Scholar
[55]

P. Saravanaraman, R.K. Chinnadurai, R. Boopathy, A new role for the nonpathogenic nonsynonymous single-nucleotide polymorphisms of acetylcholinesterase in the treatment of Alzheimer's disease: a computational study, J. Compu. Biol. 21 (2014) 632-647. https://doi.org/10.1089/cmb.2014.0005.
DOI Google Scholar
[56]

H. Zare-Zardini, B. Tolueinia, A. Hashemi, et al., Antioxidant and cholinesterase inhibitory activity of a new peptide from Ziziphus jujuba fruits, Am. J. Alzheimers Dis. 28 (2013) 702-709.https://doi.org/10.1177/1533317513500839.
DOI Google Scholar
[57]

H. Md, P. Mehnaz, L. Beong, Acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice, Molecules 18 (2013) 6663-6678.https://doi.org/10.3390/molecules18066663.
DOI Google Scholar
[58]

S.A. Malomo, R.E. Aluko, Kinetics of acetylcholinesterase inhibition by hemp seed protein-derived peptides, J. Food Biochem. 43 (2019) e12897.https://doi.org/10.1111/jfbc.12897.
DOI Google Scholar
[59]

R. Yao, L. Kai, Y. Jin, et al., Identification and characterization of two novel α-glucosidase inhibitory oligopeptides from hemp (Cannabis sativa L.) seed protein, J. Funct. Foods 26 (2016) 439-450. https://doi.org/10.1016/j.jff.2016.07.024.
DOI Google Scholar
[60]

C. Lammi, C. Bollati, F. Gelain, et al., Enhancement of the stability and anti-DPPIV activity of hempseed hydrolysates through self-assembling peptide-based hydrogels, Front. Chem. 6 (2019) 670. https://doi.org/10.3389/fchem.2018.00670.
DOI Google Scholar
[61]

L.H. Wei, Y. Dong, Y.F. Sun, et al., Anticancer property of hemp bioactive peptides in Hep3B liver cancer cells through Akt/GSK3β/β-atenin signaling pathway, Food Sci. Nutr. 9 (2021) 1833-1841. https://doi.org/10.1002/fsn3.1976.
DOI Google Scholar
[62]

N.M. Rodriguez-Martin, M. Paz, R. Toscano, et al., Hemp (Cannabis sativa L.) protein hydrolysates promote anti-inflammatory response in primary human monocytes, Biomolecules 10 (2020) 803. https://doi.org/10.3390/biom10050803.
DOI Google Scholar
[63]

N.M. Rodriguez-Martin, R. Toscano, A. Villanueva, et al., Neuroprotective protein hydrolysates from hemp (Cannabis sativa L.) seeds, Food Funct. 10(2020) 6732-6739. https://doi.org/10.1039/C9FO01904A.
DOI Google Scholar
[64]

A. Jurgoński, P.M. Opyd, B. Fotschki, Effects of native or partially defatted hemp seeds on hindgut function, antioxidant status and lipid metabolism in diet-induced obese rats, J. Funct. Foods 72 (2020) 104071.https://doi.org/10.1016/j.jff.2020.104071.
DOI Google Scholar
[65]

B. Ckta, C. Wwk, D. Chd, et al., The soybean bioactive peptide VHVV alleviates hypertension-induced renal damage in hypertensive rats via the SIRT1-PGC1α/Nrf2 pathway, J. Funct. Foods 75 (2020) 104255.https://doi.org/10.1016/j.jff.2020.104255.
DOI Google Scholar
[66]

J. Tkaczewska, Peptides and protein hydrolysates as food preservatives and bioactive components of edible films and coatings-a review, Trends Food Sci. Tech. 106 (2020) 298-311. https://doi.org/10.1016/j.tifs.2020.10.022.
DOI Google Scholar
[67]

B. Msa, A. Gh, B. Mg, Unravelling the molecular mechanisms associated with the role of food-derived bioactive peptides in promoting cardiovascular health, J. Funct. Foods 64 (2020) 103645. https://doi.org/10.1016/j.jff.2019.103645.
DOI Google Scholar
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Received: 19 December 2021
Revised: 29 January 2022
Accepted: 07 March 2022
Published: 18 November 2022
Issue date: July 2023

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© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

The financial support received from the Shenzhen Science and Technology Innovation Commission (KCXFZ20201221173207022), Youth Science Foundation Project (32101936) and China Postdoctoral Science Foundation, No.15 Special Fund (In-Station) (2022T150366) are gratefully acknowledged.

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