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Research Article | Open Access

Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff

Siyuan Sua,b,1, Xiang Dingc,1, Yiling Houa( ), Binbin Liub, Zhouhe Dub, Junfeng Liub
Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong 637009, China
Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, China
College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China

1 Authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

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Abstract

A new water-soluble heteropolysaccharide with a molecular weight of 15 kDa was isolated from the fruiting bodies of Boletus reticulatus Schaeff. Structural characterization results revealed that B. reticulatus Schaeff polysaccharide (BRS-X) had a backbone of 1,6-linked α-Dgalactose and 1,2,6-linked α-D-galactose which branches were mainly composed of a terminal 4-linked β-D-glucose and the ratio of D-galactose and D-glucose was 5:1. Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G (IgG), IgE, IgD and IgM. In addition, BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice. The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3K/Akt signaling pathways in BRS-X group. The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X. These findings provide a scientific basis for the edible and medicinal value of BRS-X.

Keywords

Boletus reticulatus SchaeffPolysaccharidesStructure identificationImmunomodulatory activityAntitumor activity

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Food Science and Human Wellness
Volume 12 Issue 2,
March 2023
Pages 647-661
DOI: 10.1016/j.fshw.2022.07.067
Cite this article:
Su S, Ding X, Hou Y, et al. Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff. Food Science and Human Wellness, 2023, 12(2): 647-661. https://doi.org/10.1016/j.fshw.2022.07.067

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Received: 24 July 2021
Revised: 17 August 2021
Accepted: 30 September 2021
Published: 07 September 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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