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Food Science and Human Wellness 2023, 12(2): 647-661 https://doi.org/10.1016/j.fshw.2022.07.067
Research Article | Open Access | Issue | Published: 07 September 2022

Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff

Show Author's Information Siyuan Sua,b,1,  Xiang Dingc,1,  Yiling Houa( ),  Binbin Liub,  Zhouhe Dub,  Junfeng Liub
Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong 637009, China
Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, China
College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China

1 Authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:
Polysaccharides, Structure identification, Boletus reticulatus Schaeff, Immunomodulatory activity, Antitumor activity
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Su S, Ding X, Hou Y, et al. Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff. Food Science and Human Wellness, 2023, 12(2): 647-661. https://doi.org/10.1016/j.fshw.2022.07.067
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Abstract Full text About this article

A new water-soluble heteropolysaccharide with a molecular weight of 15 kDa was isolated from the fruiting bodies of Boletus reticulatus Schaeff. Structural characterization results revealed that B. reticulatus Schaeff polysaccharide (BRS-X) had a backbone of 1,6-linked α-Dgalactose and 1,2,6-linked α-D-galactose which branches were mainly composed of a terminal 4-linked β-D-glucose and the ratio of D-galactose and D-glucose was 5:1. Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G (IgG), IgE, IgD and IgM. In addition, BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice. The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3K/Akt signaling pathways in BRS-X group. The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X. These findings provide a scientific basis for the edible and medicinal value of BRS-X.


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About this article

Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff

Show Author's information Siyuan Sua,b,1, Xiang Dingc,1, Yiling Houa( ), Binbin Liub, Zhouhe Dub, Junfeng Liub
Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong 637009, China
Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, China
College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China

1 Authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

A new water-soluble heteropolysaccharide with a molecular weight of 15 kDa was isolated from the fruiting bodies of Boletus reticulatus Schaeff. Structural characterization results revealed that B. reticulatus Schaeff polysaccharide (BRS-X) had a backbone of 1,6-linked α-Dgalactose and 1,2,6-linked α-D-galactose which branches were mainly composed of a terminal 4-linked β-D-glucose and the ratio of D-galactose and D-glucose was 5:1. Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G (IgG), IgE, IgD and IgM. In addition, BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice. The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3K/Akt signaling pathways in BRS-X group. The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X. These findings provide a scientific basis for the edible and medicinal value of BRS-X.

Keywords: Polysaccharides, Structure identification, Boletus reticulatus Schaeff, Immunomodulatory activity, Antitumor activity

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Acknowledgements
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Publication history

Received: 24 July 2021
Revised: 17 August 2021
Accepted: 30 September 2021
Published: 07 September 2022
Issue date: March 2023

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© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

This project was supported by the Open Project Program of Irradiation Preservation Technology Key Laboratory of Sichuan Province, Sichuan Institute of Atomic Energy (FZBC2020009), the Open Research Fund Program of Departmental and Municipal Co-construction of Crops Genetic Improvement of Hill Land Key Laboratory of Sichuan Province (2021CGIHL02), Science and Technology Support Project of Nanchong Science and Technology Bureau of Sichuan Province (20YFZJ0053 and 20YFZJ0054), and the Sericulture Innovation Team of Sichuan Province (SCCXTD-2021-17).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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