Physicochemical properties and biological activities of polysaccharides from the peel of <i>Dioscorea opposita</i> Thunb. extracted by four different methods

Zuowei Zhao; Li Wang; Yuan Ruan; Chunnan Wen; Menghuan Ge; Yanyan Qian; Bingji Ma

doi:10.1016/j.fshw.2022.07.031

Food Science and Human Wellness 2023, 12(1): 130-139 https://doi.org/10.1016/j.fshw.2022.07.031

Research Article |

Open Access | Issue | Published: 09 August 2022

Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods

Show Author's Information Hide Author's Information Zuowei Zhao, Li Wang(

), Yuan Ruan, Chunnan Wen, Menghuan Ge, Yanyan Qian, Bingji Ma(

)

Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:

Polysaccharides, Dioscorea opposita Thunb peel, Different extraction methods, Physicochemical characteristics, Biological activities

Cite this article:

Zhao Z, Wang L, Ruan Y, et al. Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods. Food Science and Human Wellness, 2023, 12(1): 130-139. https://doi.org/10.1016/j.fshw.2022.07.031

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Abstract Full text About this article

Abstract

Polysaccharides are the important biologically active components found in the peel of Dioscorea opposita Thunb. (DTTP). The influences of 4 extraction methods, namely hot water extraction (W), acidic extraction (HA), hot-compressed water extraction (HCW) and enzyme-assisted extraction (EAE), on the yields, physicochemical properties, hypoglycemic and antioxidant activities of polysaccharides from DTTP were studied and compared. Among these DTTP polysaccharides, DTTP-HA was outstanding in several respects. DTTP-HA was the most water soluble; it had the highest total carbohydrate content (85.08%), the highest uronic acid (13.20%) and the highest thermal stability. DTTP-HA and DTTP-W possessed a triple-helix structure. These 4 kinds of polysaccharides have the same types of monosaccharides, but in different molar percentages. Extraction method had a significant impact on the microstructures of the extracted polysaccharides. DTT-HA exhibited irregular structure with many holes. Among the 4 extracted methods, the DTTP-HA and DTTP-W initially exhibited higher hypoglycemic and antioxidant activities. The better bioactivities of DTTP-HA may be related to the above factors. The findings indicated that acid extraction is an effective method to extract polysaccharides with high biological activities from DTTP.

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About this article

Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods

Show Author's information Hide Author's Information Zuowei Zhao, Li Wang(

), Yuan Ruan, Chunnan Wen, Menghuan Ge, Yanyan Qian, Bingji Ma(

)

Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Keywords: Polysaccharides, Dioscorea opposita Thunb peel, Different extraction methods, Physicochemical characteristics, Biological activities

References(37)

[1]

W.H. Yang, Z.L. Shi, Z.F. Bai, et al., High content screening for promoting effect of Dioscorea opposita on macrophage phagocytosis, Chin. Tradit. Herb, Drugs 48 (2017) 1604-1610. https://doi.org/10.7501/j.issn.0253-2670.2017.08.019.

DOI Google Scholar

[2]

S.J. Wang, H.Y. Liu, W.Y. Gao, et al., Characterization of new starches separated from different Chinese yam (Dioscorea opposita Thunb.) cultivars, Food Chem. 99 (1) (2006) 30-37. https://doi.org/10.1016/j.foodchem.2005.07.008.

DOI Google Scholar

[3]

X.M. Yun, S. Dong, Q.Q. Hu, et al., ¹H NMR-based metabolomics approach to investigate the urine samples of collagen-induced arthritis rats and the intervention of tetrandrine, J. Pharmaceut. Biomed. Anal. 154 (2018) 302-311. https://doi.org/10.1016/j.jpba.2018.03.026.

DOI Google Scholar

[4]

A. Aipire, P.F. Yuan, A. Aimaier, et al., Preparation, characterization, and immuno-enhancing activity of polysaccharides from Glycyrrhiza uralensis, Biomolecules 10 (1) (2020) 159. https://doi.org/10.3390/biom10010159.

DOI Google Scholar

[5]

X.L. Ji, C.Y. Hou, Y.Z. Yan, et al., Comparison of structural characterization and antioxidant activity of polysaccharides from jujube (Ziziphus jujuba Mill.) fruit, Int. J. Biol. Macromol. 149 (2020) 1008-1018. https://doi.org/10.1016/j.ijbiomac.2020.02.018.

DOI Google Scholar

[6]

L. An, Y.L. Yuan, J.W. Ma, et al., NMR-based metabolomics approach to investigate the distribution characteristics of metabolites in Dioscorea opposita Thunb. cv. Tiegun, Food Chem. 298 (2019) 125063. https://doi.org/10.1016/j.foodchem.2019.125063.

DOI Google Scholar

[7]

Z.Y. Zhu, F.Y. Dong, X.C. Liu, et al., Effects of extraction methods on the yield, chemical structure and anti-tumor activity of polysaccharides from Cordyceps gunnii mycelia, Carbohydr Polym. 140 (2016) 461-471. https://doi.org/10.1016/j.carbpol.2015.12.053.

DOI Google Scholar

[8]

H.M. Shang, S.L. Chen, R. Li, et al., Influences of extraction methods on physicochemical characteristics and activities of Astragalus cicer L. polysaccharides, Process Biochem. 73 (2018) 220-227. https://doi.org/10.1016/j.procbio.2018.07.016.

DOI Google Scholar

[9]

S.Y. Jia, F. Li, Y. Liu, et al., Effects of extraction methods on the antioxidant activities of polysaccharides from Agaricus blazei Murrill, Int. J. Biol. Macromol. 62 (2013) 66-69. https://doi.org/10.1016/j.ijbiomac.2013.08.031.

DOI Google Scholar

[10]

L. Wang, H. Liu, G. Qin, Structure characterization and antioxidant activity of polysaccharides from Chinese quince seed meal, Food Chem. 234 (2017) 314-322. https://doi.org/10.1016/j.foodchem.2017.05.002.

DOI Google Scholar

[11]

C. Thetsrimuang, S. Khammuang, K. Chiablaem, et al., Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév, Food Chem. 128 (3) (2011) 634-639. https://doi.org/10.1016/j.foodchem.2011.03.077.

DOI Google Scholar

[12]

X.T. Wang, Z.Y. Zhu, L. Zhao, et al., Structural characterization and inhibition on α-D-glucosidase activity of non-starch polysaccharides from Fagopyrum tartaricum, Carbohydr. Polym. 153 (2016) 679-685. https://doi.org/10.1016/j.carbpol.2016.08.024.

DOI Google Scholar

[13]

F. Xiong, X. Li, L.H. Zheng, et al., Characterization and antioxidant activities of polysaccharides from Passiflora edulis Sims peel under different degradation methods, Carbohydr Polym. 218 (2019) 46-52. https://doi.org/10.1016/j.carbpol.2019.04.069.

DOI Google Scholar

[14]

H.C. Liu, H.X. Fan, J. Zhang, et al., Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don, Food Sci. Hum. Well. 9 (2020) 71-79. https://doi.org/10.1016/j.fshw.2019.12.006.

DOI Google Scholar

[15]

J.Q. Qiu, H. Zhang, Z.Y. Wang, Ultrasonic degradation of polysaccharides from Auricularia auricula and the antioxidant activity of their degradation products, LWT-Food Sci. Tech. 113 (2019) 108266. https://doi.org/10.1016/j.lwt.2019.108266.

DOI Google Scholar

[16]

C.J. Gao, Y.H. Wang, C.Y. Wang, et al., Antioxidant and immunological activity in vitro of polysaccharides from Gomphidius rutilus mycelium, Carbohydr Polym. 92 (2) (2013) 2187-2192. https://doi.org/10.1016/j.carbpol.2012.12.011.

DOI Google Scholar

[17]

M.Y. Duan, H.M. Shang, S.L. Chen, et al., Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques, Int. J. Biol. Macromol. 115 (2018) 876-882. https://doi.org/10.1016/j.ijbiomac.2018.04.188.

DOI Google Scholar

[18]

S.A. Ahmed, S.A. EI-Mahdy, A.M. Hashem, et al., Functional change of Bacillus acidocaldarius α-amylase chemically modified with periodate oxidized polysaccharides, Biocatal. Agric. Biotechnol. 16 (2018) 73-78. https://doi.org/10.1016/j.bcab.2018.07.020.

DOI Google Scholar

[19]

Y. Chi, Y. Li, G. Zhang, et al., Effect of extraction techniques on properties of polysaccharides from Enteromorpha prolifera and their applicability in iron chelation, Carbohydr Polym. 181 (2018) 616-623. https://doi.org/10.1016/j.carbpol.2017.11.104.

DOI Google Scholar

[20]

Z.B. Wang, J.J. Pei, H.L. Ma, et al., Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides, Carbohydr. Polym. 109 (2014) 49-55. https://doi.org/10.1016/j.carbpol.2014.03.057.

DOI Google Scholar

[21]

DOI Google Scholar

[22]

L. He, X.T. Yan, J. Liang, et al., Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem, Carbohydr. Polym. 198 (2018) 101-108. https://doi.org/10.1016/j.carbpol.2018.06.073.

DOI Google Scholar

[23]

J.Y. Qian, W. Chen, W.M. Zhang, et al., Adulteration identification of some fungal polysaccharides with SEM, XRD, IR and optical rotation: A primary approach, Carbohydr. Polym. 78 (3) (2009) 620-625. https://doi.org/10.1016/j.carbpol.2009.05.025.

DOI Google Scholar

[24]

R.F. Wang, P. Chen, F. Jia, et al., Optimization of polysaccharides from Panax japonicus C.A. Meyer by RSM and its anti-oxidant activity, Int. J. Biol. Macromol. 50 (2) (2012) 331-336. https://doi.org/10.1016/j.ijbiomac.2011.12.023.

DOI Google Scholar

[25]

Z.Y. Zhu, N. Liu, C.L. Si, et al., Structure and anti-tumor activity of a high-molecular-weight polysaccharide from cultured mycelium of Cordyceps gunnii, Carbohydr. Polym. 88 (3) (2012) 1072-1076. https://doi.org/10.1016/j.carbpol.2012.01.068.

DOI Google Scholar

[26]

M. Kac̆uráková, P. Capek, V. Sasinková, et al., FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicelluloses, Carbohydr Polym. 43 (2) (2000) 195-203. https://doi.org/10.1016/S0144-8617(00)00151-X.

DOI Google Scholar

[27]

Y. Li, G.Y.X. Qin, C. Cheng, et al., Purification, characterization and anti-tumor activities of polysaccharides from Ecklonia kurome obtained by three different extraction methods, Int. J, Biol. Macromol. 150 (2020) 1000-1010. https://doi.org/10.1016/j.ijbiomac.2019.10.216.

DOI Google Scholar

[28]

L. Wang, P.Z. Zhang, J.W. Shen, et al., Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus, Int. J. Biol. Macromol. 141 (2019) 150-160. https://doi.org/10.1016/j.ijbiomac.2019.08.250.

DOI Google Scholar

[29]

Y.H. Xiao, S.C. Liu, M.Y. Shen, et al., Physicochemical, rheological and thermal properties of Mesona chinensis polysaccharides obtained by sodium carbonate assisted and cellulase assisted extraction, Int. J. Biol Macromol. 126 (2019) 30-36. https://doi.org/10.1016/j.ijbiomac.2018.12.211.

DOI Google Scholar

[30]

Z. Qin, H.M. Liu, T.T. Lv, et al., Structure, rheological, thermal and antioxidant properties of cell wall polysaccharides from Chinese quince fruits, Int. J. Biol. Macromol. 147 (2020) 1146-1155. https://doi.org/10.1016/j.ijbiomac.2019.10.083.

DOI Google Scholar

[31]

J.C. Chen, X. Zhang, D. Huo, et al., Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus, Carbohydr Polym. 215 (2019) 307-315. https://doi.org/10.1016/j.carbpol.2019.03.099.

DOI Google Scholar

[32]

J.Y. Gu, H.H. Zhang, H. Yao, et al., Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L, Carbohydr Polym. 235 (2020) 115939. https://doi.org/10.1016/j.carbpol.2020.115939.

DOI Google Scholar

[33]

F.Y. Ma, D.K. Wang, Y. Zhang, et al., Characterisation of the mucilage polysaccharides from Dioscorea opposita Thunb. with enzymatic hydrolysis, Food Chem. 245 (2018) 13-21. https://doi.org/10.1016/j.foodchem.2017.10.080.

DOI Google Scholar

[34]

J.K. Yan, L.X. Wu, W.D. Cai, et al., Subcritical water extraction-based methods affect the physicochemical and functional properties of soluble dietary fibers from wheat bran, Food Chem. 298 (2019) 124987. https://doi.org/10.1016/j.foodchem.2019.124987.

DOI Google Scholar

[35]

J.K. Yan, Z.C. Ding, X.L. Gao, et al., Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions, Carbohydr. Polym. 193 (2018) 373-382. https://doi.org/10.1016/j.carbpol.2018.04.019.

DOI Google Scholar

[36]

J.X. Song, Y. Wu, X.J. Ma, et al., Structural characterization and α-glycosidase inhibitory activity of a novel polysaccharide fraction from Aconitum coreanum, Carbohydr. Polym. 230 (2020) 115586. https://doi.org/10.1016/j.carbpol.2019.115586.

DOI Google Scholar

[37]

C.C. Fang, G.J. Chen, J.Q. Kan, Comparison on characterization and biological activities of Mentha haplocalyx polysaccharides at different solvent extractions, Int. J. Biol. Macromol. 154 (2020) 916-928. https://doi.org/10.1016/j.ijbiomac.2020.03.169.

DOI Google Scholar

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Publication history

Received: 27 January 2021

Revised: 07 February 2021

Accepted: 09 April 2021

Published: 09 August 2022

Issue date: January 2023

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The authors gratefully acknowledge the financial support provided by Zhengzhou 1125 Talents Gathering Project (2018) and the Doctor startup fund of Henan Agricultural University (30500431).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).