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Food Science and Human Wellness 2022, 11(6): 1580-1590 https://doi.org/10.1016/j.fshw.2022.06.016
Research Article | Open Access | Issue | Published: 18 July 2022

Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) protein-derived antioxidant peptides: mechanisms of action and structure-activity relationship in Caco-2 cell models

Show Author's Information Yiming Zhoua,  Xuanming Shea,  Zhidong Chena,  Yun Weia,  Ying Xiaoa,  Xiaoli Zhoua,b( )
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China
University Think Tank of Shanghai Municipality, Institute of Beautiful China and Ecological Civilization, Shanghai 201418, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Antioxidant protective, Tartary buckwheat peptides, Metabolic mechanim, Caco-2 cells
Cite this article:
Zhou Y, She X, Chen Z, et al. Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) protein-derived antioxidant peptides: mechanisms of action and structure-activity relationship in Caco-2 cell models. Food Science and Human Wellness, 2022, 11(6): 1580-1590. https://doi.org/10.1016/j.fshw.2022.06.016
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Abstract Full text About this article

Excessive reactive oxygen species (ROS) can cause oxidative damage and lead to various metabolic disease. Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) is a new kind of protein-rich functional food, the protein in which has been proved to have good antioxidant capacity. In this study, in order to further explore the antioxidant mechanism of Tartary buckwheat protein, 4 peptides (CR-8, LR-8, GK-10 and SR-12) were isolated and identified from it. H2O2 was used to induce oxidative damage to Caco-2 cells to evaluate antioxidant capacity of these peptides. The results of superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and mitochondrial membrane potential etc. showed that these peptides have superior antioxidant capacity. CR-8 has the best antioxidant capacity. In order to further clarify the antioxidant mechanism of CR-8, metabolomics was used to analyze related metabolites and metabolic pathways. The results showed that after CR-8 intervention, the content of metabolites such as L-acetyl carnitine has increased. This indicated that CR-8 can improve the antioxidant capacity of damaged cells by intervening in multiple metabolic pathways. This also revealed the anti-oxidant mechanism of tartary buckwheat protein. In conclusion, it provided a theoretical basis for further studying the activity of tartary buckwheat portein and utilizing buckwheat resources.


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About this article

Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) protein-derived antioxidant peptides: mechanisms of action and structure-activity relationship in Caco-2 cell models

Show Author's information Yiming Zhoua, Xuanming Shea, Zhidong Chena, Yun Weia, Ying Xiaoa, Xiaoli Zhoua,b( )
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China
University Think Tank of Shanghai Municipality, Institute of Beautiful China and Ecological Civilization, Shanghai 201418, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Excessive reactive oxygen species (ROS) can cause oxidative damage and lead to various metabolic disease. Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) is a new kind of protein-rich functional food, the protein in which has been proved to have good antioxidant capacity. In this study, in order to further explore the antioxidant mechanism of Tartary buckwheat protein, 4 peptides (CR-8, LR-8, GK-10 and SR-12) were isolated and identified from it. H2O2 was used to induce oxidative damage to Caco-2 cells to evaluate antioxidant capacity of these peptides. The results of superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and mitochondrial membrane potential etc. showed that these peptides have superior antioxidant capacity. CR-8 has the best antioxidant capacity. In order to further clarify the antioxidant mechanism of CR-8, metabolomics was used to analyze related metabolites and metabolic pathways. The results showed that after CR-8 intervention, the content of metabolites such as L-acetyl carnitine has increased. This indicated that CR-8 can improve the antioxidant capacity of damaged cells by intervening in multiple metabolic pathways. This also revealed the anti-oxidant mechanism of tartary buckwheat protein. In conclusion, it provided a theoretical basis for further studying the activity of tartary buckwheat portein and utilizing buckwheat resources.

Keywords: Antioxidant protective, Tartary buckwheat peptides, Metabolic mechanim, Caco-2 cells

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Publication history

Received: 13 March 2021
Revised: 28 April 2021
Accepted: 19 June 2021
Published: 18 July 2022
Issue date: November 2022

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© 2022 Beijing Academy of Food Sciences.

Acknowledgements

Acknowledgements

The authors thank Shanghai Natural Science Foundation (20ZR1455800), the National Science Foundation of China (31871805), China Agriculture Research System (CARS-08-D2) and Shanghai Municipal Education Commission (Plateau Discipline Construction Program).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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