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Food Science and Human Wellness 2022, 11(6): 1527-1538 https://doi.org/10.1016/j.fshw.2022.06.010
Research Article | Open Access | Issue | Published: 18 July 2022

The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway

Show Author's Information Jiaying Huoa,b,  Yuezhang Mingc,  Huifeng Lic,  Anjun Lid,  Jiwen Zhaoe,  Mingquan Huanga( ),  Weizheng Sunb,  Jihong Wua( ),  Jinglin Zhanga
Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, China
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
Inner Mongolia Taibusiqi Grassland King Wine Co., Ltd., Inner Mongolia 027000, China
Anhui Gujing Distillery Co., Ltd., Anhui 236000, China
Shandong Bandaojing Co., Ltd., Shandong 256300, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
HepG2 cells, Chinese baijiu, High-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, Antioxidant peptides, Nrf2 signaling pathway
Cite this article:
Huo J, Ming Y, Li H, et al. The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway. Food Science and Human Wellness, 2022, 11(6): 1527-1538. https://doi.org/10.1016/j.fshw.2022.06.010
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Abstract Full text About this article

Antioxidant peptides have been widely reported. However, only a few reports have been published examining the antioxidant peptides derived from Chinese baijiu. In this study, 6 novel peptides derived from Chinese baijiu were identified successfully using high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (HPLC-QTOF-MS) with a concentration of 0.835–24.540 µg/L. The underlying molecular mechanisms were investigated, and their cytoprotective effects were examined against 2,2’-azobis (2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells. The results showed that these peptides exerted protective effects by suppressing reactive oxygen species (ROS) generation, preventing malondialdehyde (MDA) formation, and upregulating cellular antioxidant enzyme activities (SOD, CAT, and GSH-Px) in a dose-dependent manner. Further experiments proved that these peptides exerted antioxidant effects via Nrf2/ARE-mediated signaling pathway by promoting Nrf2 nuclear translocation, inhibiting ubiquitination, and enhancing transcription capacity of Nrf2 in HepG2 cells. These findings provide the molecular basis for the effects of antioxidant peptides derived from Chinese baijiu, which is important for a deeper understanding of the relationship between human health and moderate drinking.


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About this article

The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway

Show Author's information Jiaying Huoa,b, Yuezhang Mingc, Huifeng Lic, Anjun Lid, Jiwen Zhaoe, Mingquan Huanga( ), Weizheng Sunb, Jihong Wua( ), Jinglin Zhanga
Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, China
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
Inner Mongolia Taibusiqi Grassland King Wine Co., Ltd., Inner Mongolia 027000, China
Anhui Gujing Distillery Co., Ltd., Anhui 236000, China
Shandong Bandaojing Co., Ltd., Shandong 256300, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Antioxidant peptides have been widely reported. However, only a few reports have been published examining the antioxidant peptides derived from Chinese baijiu. In this study, 6 novel peptides derived from Chinese baijiu were identified successfully using high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (HPLC-QTOF-MS) with a concentration of 0.835–24.540 µg/L. The underlying molecular mechanisms were investigated, and their cytoprotective effects were examined against 2,2’-azobis (2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells. The results showed that these peptides exerted protective effects by suppressing reactive oxygen species (ROS) generation, preventing malondialdehyde (MDA) formation, and upregulating cellular antioxidant enzyme activities (SOD, CAT, and GSH-Px) in a dose-dependent manner. Further experiments proved that these peptides exerted antioxidant effects via Nrf2/ARE-mediated signaling pathway by promoting Nrf2 nuclear translocation, inhibiting ubiquitination, and enhancing transcription capacity of Nrf2 in HepG2 cells. These findings provide the molecular basis for the effects of antioxidant peptides derived from Chinese baijiu, which is important for a deeper understanding of the relationship between human health and moderate drinking.

Keywords: HepG2 cells, Chinese baijiu, High-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, Antioxidant peptides, Nrf2 signaling pathway

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Publication history

Received: 26 September 2020
Revised: 30 November 2020
Accepted: 31 January 2021
Published: 18 July 2022
Issue date: November 2022

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© 2022 Beijing Academy of Food Sciences.

Acknowledgements

Acknowledgements

This work was supported by National Key Research & Development Program of China (2017YFC1600401-3) and National Natural Science Foundation of China (31871749 and 31701567).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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