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Food Science and Human Wellness 2022, 11(6): 1482-1490 https://doi.org/10.1016/j.fshw.2022.06.005
Research Article | Open Access | Issue | Published: 18 July 2022

Nutritional properties of Europen eel (Anguilla anguilla) bone peptide-calcium and its apoptosis effect on Caco-2 cells

Show Author's Information Hui Tenga,b,1,  Yuewei Qianb,1,  Xiaoyun Fana,  Hui Caoa( ),  Yuting Tiana( ),  Lei Chena,b( )
Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

1 Authors contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Calcium, Peptide, Apoptosis, Europen eel bone, Nutritional properties, Caco-2
Cite this article:
Teng H, Qian Y, Fan X, et al. Nutritional properties of Europen eel (Anguilla anguilla) bone peptide-calcium and its apoptosis effect on Caco-2 cells. Food Science and Human Wellness, 2022, 11(6): 1482-1490. https://doi.org/10.1016/j.fshw.2022.06.005
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Abstract Full text About this article

The present study aimed at making a rational usage for European eel bone by-products by preparing Europen eel bone peptide chelated calcium (EBPC-Ca). Nutritional properties and bioactivity of EBPC-Ca were evaluated. Results showed that nutritional properties of calcium ions will cause intra- and inter-molecular folding and aggregation of peptide to uniformly form EBPC-Ca chelate. The chelated compound of EBPC and calcium ion triggered a strong apoptosis in heterogeneous human epithelial colorectal adenocarcinoma (Caco-2) in concentration- and time-dependent manners. Western blot analysis revealed that the EBPC-Ca induced apoptosis may be the result of a blocked autophagy flux through mitochondrial-dependent pathway. Additionally, the increase in FGF-23 protein expression inhibited the absorption of calcium ions and alleviated cell apoptosis. It was also found that the cell apoptosis occurs with significant increases in the levels of reactive oxygen species (ROS) and Ca2+ in the cells, indicating the anti-tumor potential of EBPC-Ca may involve multiple channels.


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About this article

Nutritional properties of Europen eel (Anguilla anguilla) bone peptide-calcium and its apoptosis effect on Caco-2 cells

Show Author's information Hui Tenga,b,1, Yuewei Qianb,1, Xiaoyun Fana, Hui Caoa( ), Yuting Tiana( ), Lei Chena,b( )
Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

1 Authors contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

The present study aimed at making a rational usage for European eel bone by-products by preparing Europen eel bone peptide chelated calcium (EBPC-Ca). Nutritional properties and bioactivity of EBPC-Ca were evaluated. Results showed that nutritional properties of calcium ions will cause intra- and inter-molecular folding and aggregation of peptide to uniformly form EBPC-Ca chelate. The chelated compound of EBPC and calcium ion triggered a strong apoptosis in heterogeneous human epithelial colorectal adenocarcinoma (Caco-2) in concentration- and time-dependent manners. Western blot analysis revealed that the EBPC-Ca induced apoptosis may be the result of a blocked autophagy flux through mitochondrial-dependent pathway. Additionally, the increase in FGF-23 protein expression inhibited the absorption of calcium ions and alleviated cell apoptosis. It was also found that the cell apoptosis occurs with significant increases in the levels of reactive oxygen species (ROS) and Ca2+ in the cells, indicating the anti-tumor potential of EBPC-Ca may involve multiple channels.

Keywords: Calcium, Peptide, Apoptosis, Europen eel bone, Nutritional properties, Caco-2

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Publication history

Received: 29 March 2021
Revised: 27 April 2021
Accepted: 28 May 2021
Published: 18 July 2022
Issue date: November 2022

Copyright

© 2022 Beijing Academy of Food Sciences.

Acknowledgements

This work is supported by National Natural Science Foundation of China (NSFC, Grant No. 31801459, Grant No. 32072209); China Postdoctoral Science Foundation Funded Project (2020M682073), the Science and Technology General Projects of Fujian Province (2019J01393). We are grateful to the Program of Innovative Research Team in Science and Technology in Fujian Province University ([2020]12), for providing the equipment used in this research.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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