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Food Science and Human Wellness 2022, 11(5): 1355-1361 https://doi.org/10.1016/j.fshw.2022.04.035
Research Article | Open Access | Issue | Published: 02 June 2022

Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice

Show Author's Information Yangwenjie Wanga,1,  Yang Xiangb,1,  Ruiqi Wanga,  Xiangning Lia,  Jianxiong Wangc,  Siwang Yud,  Ying Zhanga( )
School of Sport Science, Beijing Sport University, Beijing 100084, China
College of Physical Education, Yan'an University, Shaanxi 716000, China
Faculty of Health, Engineering, and Sciences, University of Southern Queensland, Toowoomba 4350, Australia
State Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, Peking University School of Pharmaceutical Sciences, Beijing 100191, China

1 The authors have contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Antioxidant, Mice, Sulforaphane, Skeletal muscle, Nrf2 (nuclear factor erythroid-derived 2-like 2), HIIT (high-intensity interval training)
Cite this article:
Wang Y, Xiang Y, Wang R, et al. Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice. Food Science and Human Wellness, 2022, 11(5): 1355-1361. https://doi.org/10.1016/j.fshw.2022.04.035
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Abstract Full text About this article

Nuclear factor erythroid-derived 2-like 2 (Nrf2) is the master regulator of antioxidant defenses. High-intensity interval training (HIIT) has been proposed as a time-efficient training program and has become a substantial component of modern training program. In the present study, we evaluated the effects of sulforaphane (SFN), a dietary isothiocyanate derived from cruciferous vegetables and a potent Nrf2 activator, on Nrf2-mediated antioxidant defense responses of skeletal muscle induced by exhaustive exercise in HIIT mice. Male C57BL/6J mice were randomly allocated into control group, HIIT group, and HIIT pretreated with SFN (HIIT+SFN) group. On the third day after completion of a 6-weeks HIIT protocol, an exhaustive treadmill test was conducted in all mice. Mice were intraperitoneally injected with SFN (HIIT+SFN group) or PBS (HIIT and control mice) 4 times in 3 days prior to the exhaustive treadmill test. The results indicated that the 6-weeks HIIT protocol did not increase the antioxidative capacity of skeletal muscle during exhaustive exercise. Importantly, SFN treatment improved antioxidative capacity of skeletal muscle in response to the acute exhaustive exercise by increasing mRNA and nucleoprotein expression of Nrf2 and these genes involved in antioxidant generation and decreasing blood creatine kinase (CK) and 4-hydroxy-2-nonenal (4-HNE)-modified protein levels in the HIIT mice.


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Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice

Show Author's information Yangwenjie Wanga,1, Yang Xiangb,1, Ruiqi Wanga, Xiangning Lia, Jianxiong Wangc, Siwang Yud, Ying Zhanga( )
School of Sport Science, Beijing Sport University, Beijing 100084, China
College of Physical Education, Yan'an University, Shaanxi 716000, China
Faculty of Health, Engineering, and Sciences, University of Southern Queensland, Toowoomba 4350, Australia
State Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, Peking University School of Pharmaceutical Sciences, Beijing 100191, China

1 The authors have contributed equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Nuclear factor erythroid-derived 2-like 2 (Nrf2) is the master regulator of antioxidant defenses. High-intensity interval training (HIIT) has been proposed as a time-efficient training program and has become a substantial component of modern training program. In the present study, we evaluated the effects of sulforaphane (SFN), a dietary isothiocyanate derived from cruciferous vegetables and a potent Nrf2 activator, on Nrf2-mediated antioxidant defense responses of skeletal muscle induced by exhaustive exercise in HIIT mice. Male C57BL/6J mice were randomly allocated into control group, HIIT group, and HIIT pretreated with SFN (HIIT+SFN) group. On the third day after completion of a 6-weeks HIIT protocol, an exhaustive treadmill test was conducted in all mice. Mice were intraperitoneally injected with SFN (HIIT+SFN group) or PBS (HIIT and control mice) 4 times in 3 days prior to the exhaustive treadmill test. The results indicated that the 6-weeks HIIT protocol did not increase the antioxidative capacity of skeletal muscle during exhaustive exercise. Importantly, SFN treatment improved antioxidative capacity of skeletal muscle in response to the acute exhaustive exercise by increasing mRNA and nucleoprotein expression of Nrf2 and these genes involved in antioxidant generation and decreasing blood creatine kinase (CK) and 4-hydroxy-2-nonenal (4-HNE)-modified protein levels in the HIIT mice.

Keywords: Antioxidant, Mice, Sulforaphane, Skeletal muscle, Nrf2 (nuclear factor erythroid-derived 2-like 2), HIIT (high-intensity interval training)

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Received: 11 December 2020
Revised: 06 January 2021
Accepted: 02 February 2021
Published: 02 June 2022
Issue date: September 2022

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© 2022 Beijing Academy of Food Sciences.

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