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Food Science and Human Wellness 2022, 11(4): 854-864 https://doi.org/10.1016/j.fshw.2022.03.007
Research Article | Open Access | Issue | Published: 28 April 2022

Transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis during development of Korla fragrant pear in Xinjiang

Show Author's Information Hao Wena,  Wenqiang Wanga,  Xi Jiangb,  Minyu Wua,  Hongjin Baib,  Cuiyun Wub( ),  Lirong Shena( )
Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China
College of Horticulture Forestry, Tarim University of Land-Reclamation, Aral 843300, China
Keywords:
Korla fragrant pear, Development period, Chlorogenic acid content, De novo transcriptome assembly, Phenylpropanoid biosynthesis pathway
Cite this article:
Wen H, Wang W, Jiang X, et al. Transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis during development of Korla fragrant pear in Xinjiang. Food Science and Human Wellness, 2022, 11(4): 854-864. https://doi.org/10.1016/j.fshw.2022.03.007
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Abstract Full text About this article

Korla fragrant pear (KFP) with special fragrance is a unique cultivar in Xinjiang, China. In order to explore the biosynthesis molecular mechanism of chlorogenic acid (CGA) in KFP, the samples at different development periods were collected for transcriptome analysis. High performance liquid chromatography analysis showed that CGA contents of KFP at 88, 118 and 163 days after full bloom were (20.96 ± 1.84), (12.01 ± 0.91) and (7.16 ± 0.41) mg/100 g, respectively, and decreased with the fruit development. Pears from these typical 3 periods were selected for de novo transcriptome assemble and 68059 unigenes were assembled from 444037960 clean reads. One 'phenylpropanoid biosynthesis' pathway including 57 unigenes, 11 PALs, 1 PTAL, 6 4CLs, 9 C4Hs, 25 HCTs and 5 C3'Hs related to CGA biosynthesis was determined. It was found that the expression levels of 11 differentially expressed genes including 1 PAL, 2 C4Hs, 3 4CLs and 5 HCTs were consistent with the change of CGA content. Quantitative polymerase chain reaction analysis further showed that 8 unigenes involved in CGA biosynthesis were consistent with the RNA-seq data. These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in KFP.


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About this article

Transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis during development of Korla fragrant pear in Xinjiang

Show Author's information Hao Wena, Wenqiang Wanga, Xi Jiangb, Minyu Wua, Hongjin Baib, Cuiyun Wub( ), Lirong Shena( )
Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China
College of Horticulture Forestry, Tarim University of Land-Reclamation, Aral 843300, China

Abstract

Korla fragrant pear (KFP) with special fragrance is a unique cultivar in Xinjiang, China. In order to explore the biosynthesis molecular mechanism of chlorogenic acid (CGA) in KFP, the samples at different development periods were collected for transcriptome analysis. High performance liquid chromatography analysis showed that CGA contents of KFP at 88, 118 and 163 days after full bloom were (20.96 ± 1.84), (12.01 ± 0.91) and (7.16 ± 0.41) mg/100 g, respectively, and decreased with the fruit development. Pears from these typical 3 periods were selected for de novo transcriptome assemble and 68059 unigenes were assembled from 444037960 clean reads. One 'phenylpropanoid biosynthesis' pathway including 57 unigenes, 11 PALs, 1 PTAL, 6 4CLs, 9 C4Hs, 25 HCTs and 5 C3'Hs related to CGA biosynthesis was determined. It was found that the expression levels of 11 differentially expressed genes including 1 PAL, 2 C4Hs, 3 4CLs and 5 HCTs were consistent with the change of CGA content. Quantitative polymerase chain reaction analysis further showed that 8 unigenes involved in CGA biosynthesis were consistent with the RNA-seq data. These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in KFP.

Keywords: Korla fragrant pear, Development period, Chlorogenic acid content, De novo transcriptome assembly, Phenylpropanoid biosynthesis pathway

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Publication history

Received: 07 November 2020
Revised: 30 November 2020
Accepted: 16 December 2020
Published: 28 April 2022
Issue date: July 2022

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© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgement

We would like to thank Prof. Liang Liu from Department of Statistics, The University of Georgia, United States for his help on writing and revising the manuscript, Prof. Qikang Gao of Center of Analysis and Measurement, Faculty of Agriculture, Life and Environment Science, Zhejiang University for his assistance in experiment of qPCR analysis. This work was supported by Major scientific and technological projects of XPCC (2020KWZ-012).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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