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Food Science and Human Wellness 2022, 11(3): 697-710 https://doi.org/10.1016/j.fshw.2021.12.027
Research Article | Open Access | Issue | Published: 04 February 2022

Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice

Show Author's Information Kashif Hussaina,1,  Yingying Yanga,b,1,  Jie Wanga,1,  Hengjuan Biana,  Xi Leia,  Junjie Chena,  Qianying Lia,  Li Wanga,  Qingping Zhonga,  Xiang Fanga,  Yutao Wangc,  Hong Weid,  Yigang Tonge,  Zhenlin Liaoa,c( )
College of Food Science, South China Agricultural University, Guangzhou 510642, China
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada
College of Life and Geographic Sciences, Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi University, Kashi 844000, China
Institute of Precision Medicine, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Obesity, Lipid metabolism, High fat diet, Pseudo germ-free mice, Tea polyphenol
Cite this article:
Hussain K, Yang Y, Wang J, et al. Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice. Food Science and Human Wellness, 2022, 11(3): 697-710. https://doi.org/10.1016/j.fshw.2021.12.027
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Abstract Full text About this article

The role of gut microbiota in terms of host health is becoming increasingly important. In this study, the comparative effects of tea polyphenols (TPs) on weight loss and lipid metabolism on conventionalized mice (CVZ) and pseudo germ-free (PGF) mice (treated with antibiotics) were investigated. Our findings revealed that high fat (HF) diet considerably increased the body weight, total fat and upsurge lipid indices in CVZ mice but PGF mice were not sensitive to the effect of HF diet as CVZ mice. After the dietary administration of TP, body weight, perirenal fat and epididymal fat, liver weight, glucose (GLU) level, total chloestrol (TC level), high density lipoprotein-cholesterol (HDL-C) level significantly lowered in PGF mice as compared to CVZ mice group. However, the area of fat cells and triglyceride (TG) level were significantly increased in PGF mice. In CVZ mice, TP intervention resulted in a considerable drop in the Firmicutes/Bacteroides ratio as compared to PGF mice. The intestinal flora of PGF mice was severely reduced after antibiotic treatment, while TP administration restored intestinal diversity; the abundance of Akkermansia and Lactobacillus increased, whereas the abundance of Enterobacteriaceae and Prevotella reduced. Overall, we can assume that PGF obese mice administered with TP have less anti-obesity effects compared to obese CVZ mice.


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About this article

Comparative study on the weight loss and lipid metabolism by tea polyphenols in diet induced obese C57BL/6J pseudo germ free and conventionalized mice

Show Author's information Kashif Hussaina,1, Yingying Yanga,b,1, Jie Wanga,1, Hengjuan Biana, Xi Leia, Junjie Chena, Qianying Lia, Li Wanga, Qingping Zhonga, Xiang Fanga, Yutao Wangc, Hong Weid, Yigang Tonge, Zhenlin Liaoa,c( )
College of Food Science, South China Agricultural University, Guangzhou 510642, China
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada
College of Life and Geographic Sciences, Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi University, Kashi 844000, China
Institute of Precision Medicine, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

The role of gut microbiota in terms of host health is becoming increasingly important. In this study, the comparative effects of tea polyphenols (TPs) on weight loss and lipid metabolism on conventionalized mice (CVZ) and pseudo germ-free (PGF) mice (treated with antibiotics) were investigated. Our findings revealed that high fat (HF) diet considerably increased the body weight, total fat and upsurge lipid indices in CVZ mice but PGF mice were not sensitive to the effect of HF diet as CVZ mice. After the dietary administration of TP, body weight, perirenal fat and epididymal fat, liver weight, glucose (GLU) level, total chloestrol (TC level), high density lipoprotein-cholesterol (HDL-C) level significantly lowered in PGF mice as compared to CVZ mice group. However, the area of fat cells and triglyceride (TG) level were significantly increased in PGF mice. In CVZ mice, TP intervention resulted in a considerable drop in the Firmicutes/Bacteroides ratio as compared to PGF mice. The intestinal flora of PGF mice was severely reduced after antibiotic treatment, while TP administration restored intestinal diversity; the abundance of Akkermansia and Lactobacillus increased, whereas the abundance of Enterobacteriaceae and Prevotella reduced. Overall, we can assume that PGF obese mice administered with TP have less anti-obesity effects compared to obese CVZ mice.

Keywords: Obesity, Lipid metabolism, High fat diet, Pseudo germ-free mice, Tea polyphenol

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Publication history

Received: 24 April 2021
Revised: 06 October 2021
Accepted: 08 October 2021
Published: 04 February 2022
Issue date: May 2022

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© 2022 Beijing Academy of Food Sciences.

Acknowledgements

Acknowledgement

The work was financially support by the Key-Area Research and Development Program of Guangdong Province (2020B020226008, 2018b020206001), the National Science Foundation of China (NSFC31171673).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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