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Food Science and Human Wellness 2022, 11(2): 383-392 https://doi.org/10.1016/j.fshw.2021.11.018
Research Article | Open Access | Issue | Published: 25 November 2021

Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane

Show Author's Information Qun Huanga,b,c,1,  Ran Yangb,1,  Qia Wangb,  Hui Tengb,  Hongbo Songb,  Fang Gengc( ),  Peng Luoa( )
School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Meat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan 610106, China

1 Theses authors contributed equally to this article. Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:
Transcriptome, Incubation, Chorioallantoic membrane, Calcium transport
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Huang Q, Yang R, Wang Q, et al. Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane. Food Science and Human Wellness, 2022, 11(2): 383-392. https://doi.org/10.1016/j.fshw.2021.11.018
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Abstract Full text About this article

Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic development, especially the calcium absorption mechanism, transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period (E) of 9, 13, 17, and 20 days. A total of 6378 differentially expressed genes (DEGs) were identified. Functional enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such as "ion transport regulation", "immune response" and "cell cycle". Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at E13. Simultaneously, the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13, and the mastoid layer was partly exposed. Therefore, it is preliminarily inferred that CAM calcium transport starts at E13, and genes such as TRPV6, S100A10, and RANKL cooperate to regulate calcium release and transport.


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About this article

Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane

Show Author's information Qun Huanga,b,c,1, Ran Yangb,1, Qia Wangb, Hui Tengb, Hongbo Songb, Fang Gengc( ), Peng Luoa( )
School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Meat Processing Key Laboratory of Sichuan Province, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan 610106, China

1 Theses authors contributed equally to this article. Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

Chorioallantoic membrane (CAM) is responsible for respiratory gas exchange, eggshell calcium transport, embryonic acid-base equilibrium, allantoic ion, and water reabsorption during avian embryonic development. To further understand the timing of CAM gene expression during chick embryonic development, especially the calcium absorption mechanism, transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period (E) of 9, 13, 17, and 20 days. A total of 6378 differentially expressed genes (DEGs) were identified. Functional enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such as "ion transport regulation", "immune response" and "cell cycle". Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at E13. Simultaneously, the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13, and the mastoid layer was partly exposed. Therefore, it is preliminarily inferred that CAM calcium transport starts at E13, and genes such as TRPV6, S100A10, and RANKL cooperate to regulate calcium release and transport.

Keywords: Transcriptome, Incubation, Chorioallantoic membrane, Calcium transport

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Publication history

Received: 25 November 2020
Revised: 30 January 2021
Accepted: 03 February 2021
Published: 25 November 2021
Issue date: March 2022

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© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgements

This study was financially supported by the Foundation of Guizhou Educational Committee (No. KY[2021]008 and No.KY[2020]014) and the National Natural Science Foundation of China (No. 31871732).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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