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Food Science and Human Wellness 2022, 11(2): 374-382 https://doi.org/10.1016/j.fshw.2021.11.016
Research Article | Open Access | Issue | Published: 25 November 2021

Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells

Show Author's Information Zhiguo Zhanga( ),  Meilin Wanga,  Shu Xingb( ),  Chen Zhangc
School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
School of Science, The Hong Kong University of Science and Technology, Hong Kong, China
Keywords:
Apoptosis, Tumor metastasis, Rose flavonoids, Human liver cancer
Cite this article:
Zhang Z, Wang M, Xing S, et al. Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells. Food Science and Human Wellness, 2022, 11(2): 374-382. https://doi.org/10.1016/j.fshw.2021.11.016
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Abstract Full text About this article

Rosa rugosa Thunb. is recognized as both medicine and edible in China. The article investigated the antitumor activity of rose flavonoids. Water-extracted rose flavonoids (RFW) and ethanol-extracted rose flavonoids (RFE) were achieved by extracting with distilled water and 70% ethanol, respectively. The effects of the two extracts on proliferation inhibition, apoptosis inducement and metastasis prevention of human HepG2 hepatocellular carcinoma cell lines were tested, via optical/fluorescence microscopy, MTT detection, Transwell assay, flow cytometry and Western blot, etc. The results indicated that rose flavonoids at low concentration (10–40 μg/mL) had a better inhibitory effect on migration and invasion of HepG2 cells in a dose-dependent manner, while rose flavonoids at high concentration (80–160 μg/mL) could induce apoptosis of HepG2 cells by up-regulating the expression of pro-apoptotic proteins p53 and Bax, and down-regulating the expression of anti-apoptotic proteins Bcl-2, leading to the functioning of caspase-3 and caspase-9. The effect of RFE at the same concentration was significantly better than that of RFW. Conclusion, this study found that rose flavonoids had a certain inhibitory effect on proliferation and metastasis of human liver cancer cells HepG2, indicating the application of rose flavonoids in preventing and treating of liver cancer.


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About this article

Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells

Show Author's information Zhiguo Zhanga( ), Meilin Wanga, Shu Xingb( ), Chen Zhangc
School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
School of Science, The Hong Kong University of Science and Technology, Hong Kong, China

Abstract

Rosa rugosa Thunb. is recognized as both medicine and edible in China. The article investigated the antitumor activity of rose flavonoids. Water-extracted rose flavonoids (RFW) and ethanol-extracted rose flavonoids (RFE) were achieved by extracting with distilled water and 70% ethanol, respectively. The effects of the two extracts on proliferation inhibition, apoptosis inducement and metastasis prevention of human HepG2 hepatocellular carcinoma cell lines were tested, via optical/fluorescence microscopy, MTT detection, Transwell assay, flow cytometry and Western blot, etc. The results indicated that rose flavonoids at low concentration (10–40 μg/mL) had a better inhibitory effect on migration and invasion of HepG2 cells in a dose-dependent manner, while rose flavonoids at high concentration (80–160 μg/mL) could induce apoptosis of HepG2 cells by up-regulating the expression of pro-apoptotic proteins p53 and Bax, and down-regulating the expression of anti-apoptotic proteins Bcl-2, leading to the functioning of caspase-3 and caspase-9. The effect of RFE at the same concentration was significantly better than that of RFW. Conclusion, this study found that rose flavonoids had a certain inhibitory effect on proliferation and metastasis of human liver cancer cells HepG2, indicating the application of rose flavonoids in preventing and treating of liver cancer.

Keywords: Apoptosis, Tumor metastasis, Rose flavonoids, Human liver cancer

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Publication history

Received: 08 October 2020
Revised: 17 January 2021
Accepted: 17 January 2021
Published: 25 November 2021
Issue date: March 2022

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© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgements

This study was supported by the natural science foundation of Shandong province ZR2017BH053 and the youth doctor cooperation foundation of Qilu University of Technology (Shandong Academy of Sciences) 2017BSH2017.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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