Effect of bacteriocin-producing <i>Pediococcus acidilactici</i> strains on the immune system and intestinal flora of normal mice

Yiteng Qiao; Zhichang Qiu; Fengwei Tian; Leilei Yu; Jianxin Zhao; Hao Zhang; Qixiao Zhai; Wei Chen

doi:10.1016/j.fshw.2021.11.008

Food Science and Human Wellness 2022, 11(2): 238-246 https://doi.org/10.1016/j.fshw.2021.11.008

Research Article |

Open Access | Issue | Published: 25 November 2021

Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice

Show Author's Information Hide Author's Information Yiteng Qiao^{^a^,^b}, Zhichang Qiu^{^c}, Fengwei Tian^{^a^,^b}, Leilei Yu^{^a^,^b}, Jianxin Zhao^{^a^,^b}, Hao Zhang^{^a^,^b^,^d^,^e}, Qixiao Zhai^{^a^,^b}, Wei Chen^{^a^,^b^,^d}(

)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China

National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China

Wuxi Translational Medicine Research Center, Jiangsu Translational Medicine, Research Institute Wuxi Branch, Wuxi 214122, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:

Regulation, Pediococcus acidilactici, Bacteriocin, Immune system, Intestinal flora

Cite this article:

Qiao Y, Qiu Z, Tian F, et al. Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice. Food Science and Human Wellness, 2022, 11(2): 238-246. https://doi.org/10.1016/j.fshw.2021.11.008

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Abstract Full text About this article

Abstract

This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice. Two P. acidilactici strains with antibacterial activity (P. acidilactici CCFM28 and CCFM18) were obtained based on the inhibition-zone assay. The produced components were identified as bacteriocins through protease treatment, pH adjustment and hydrogen peroxide treatment. Bacteriocin-producing and non-bacteriocin-producing P. acidilactici strains (P. acidilactici CCFM28, CCFM18 and NT17-3) caused significant changes in serum immune factors and intestinal flora of normal mice. After 14 days of intervention, the relative abundance of Firmicutes was significantly decreased, but that of Proteobacteria was significantly increased at the phylum level. At the genus level, the administration of three P. acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus. Furthermore, there were also different regulations on some probiotic strains, such as Bifidobacterium, Coprococcus and Akkermansia, which were closely related to the antibacterial ability of the bacteriocin and the type of strain. The results indicated that the intervention of different P. acidilactici strains could differently change the structure of intestinal flora in normal mice, which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.

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Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice

Show Author's information Hide Author's Information Yiteng Qiao^{^a^,^b}, Zhichang Qiu^{^c}, Fengwei Tian^{^a^,^b}, Leilei Yu^{^a^,^b}, Jianxin Zhao^{^a^,^b}, Hao Zhang^{^a^,^b^,^d^,^e}, Qixiao Zhai^{^a^,^b}, Wei Chen^{^a^,^b^,^d}(

)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China

National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China

Wuxi Translational Medicine Research Center, Jiangsu Translational Medicine, Research Institute Wuxi Branch, Wuxi 214122, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Keywords: Regulation, Pediococcus acidilactici, Bacteriocin, Immune system, Intestinal flora

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Publication history

Received: 26 March 2021

Revised: 02 April 2021

Accepted: 04 April 2021

Published: 25 November 2021

Issue date: March 2022

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Acknowledgements

This work was supported by the National Natural Science Foundation of China Program (32021005); the Natural Science Foundation of Jiangsu Province (BK20200084); Projects of Innovation and Development Pillar Program for Key Industries in Southern Xinjiang of Xinjiang Production and Construction Corps (2018DB002); National First Class Discipline Program of Food Science and Technology (JUFSTR20180102); the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).