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Lactic acid bacteria (LAB) fermentation is the simplest and safest way of food preservation, and the use of probiotics in yoghurt could provide dairy products with unique flavors, textures and health benefits. In this study, Lactobacillus bulgaricus, Streptococcus thermophilus, L. reuteri DSMZ 8533 and the potential probiotic strain L. plantarum A3 were used for the milk fermentation. Results found the texture properties such as hardness, consistency, and viscosity of the yoghurt were enhanced in the mixed culture condition. Furthermore, components like amino acid (leucine), vanilla (vanillin), C18:3n6 (unsaturated fatty acids) were also accumulated in L. plantarum A3 fermented yoghurt, which leads to the significant sensory profiling difference compared with the former plain yoghurt. All these results proved L. plantarum A3 is a potential probiotic strain which could enhance the sensory and nutrition profiling of the fermented milk. Future work still needs to be done on the synergistic interaction between the traditional strains and the probiotics during the fermentation process.
Lactic acid bacteria (LAB) fermentation is the simplest and safest way of food preservation, and the use of probiotics in yoghurt could provide dairy products with unique flavors, textures and health benefits. In this study, Lactobacillus bulgaricus, Streptococcus thermophilus, L. reuteri DSMZ 8533 and the potential probiotic strain L. plantarum A3 were used for the milk fermentation. Results found the texture properties such as hardness, consistency, and viscosity of the yoghurt were enhanced in the mixed culture condition. Furthermore, components like amino acid (leucine), vanilla (vanillin), C18:3n6 (unsaturated fatty acids) were also accumulated in L. plantarum A3 fermented yoghurt, which leads to the significant sensory profiling difference compared with the former plain yoghurt. All these results proved L. plantarum A3 is a potential probiotic strain which could enhance the sensory and nutrition profiling of the fermented milk. Future work still needs to be done on the synergistic interaction between the traditional strains and the probiotics during the fermentation process.
N.M. Ali, S.K. Yeap, M.H. Yusof, et al., Comparison of free amino acids, antioxidants, soluble phenolic acids, cytotoxicity and immunomodulation of fermented mung bean and soybean, J. Sci. Food. Agri. 9 (2016) 1648-1658. https://doi.org/10.1002/jsfa.7267.
K.Y. Park, J.K. Jeong, Y.E. Lee, et al., Health benefits of Kimchi (Korean fermented vegetables) as a probiotic food, J. Med. Food 17(1) (2014) 6-20. https://doi.org/10.1089/jmf.2013.3083.
M. Kleerebezemab, P. Hols, J. Hugenholtz, Lactic acid bacteria as a cell factory: rerouting of carbon metabolism in Lactococcus lactis by metabolic engineering, Enzyme Microb. Tech. 26 (2000) 9-10. https://doi.org/10.1016/S0141-0229(00)00180-0.
N. Şanlier, B.B. Gökcen, A.C. Sezgin, Health benefits of fermented foods, Crit. Rev. Food Sci. 59(3) (2019) 506-527. https://doi.org/10.1080/10408398.2017.1383355.
M. Iwasa, W. Aoi, Chapter 13-Benefits of Lactobacillus helveticus fermented milk in sports and health, Dairy in Human Health & Disease Across the Lifespan (2017) 183-195. https://doi.org/10.1016/B978-0-12-809868-4.00013-3
S. Handa, N. Sharma, In vitro study of probiotic properties of Lactobacillus plantarum F22 isolated from chhang - a traditional fermented beverage of Himachal Pradesh, India, J. Genet. Eng. Biotechnol. 14 (2016) 91-97. https://doi.org/10.1016/j.jgeb.2016.08.001.
C.S. Ranadheera, C.A. Evans, M.C. Adams, et al., Effect of dairy probiotic combinations on in vitro gastrointestinal tolerance, intestinal epithelial cell adhesion and cytokine secretion, J. Funct. Foods 8(1) (2014) 18-25. https://doi.org/10.1016/j.jff.2014.02.022.
I. Khan, S.C. Kang, Probiotic potential of nutritionally improved Lactobacillus plantarum DGK-17 isolated from Kimchi–a traditional Korean fermented food, Food Control 60 (2016) 88-94. https://doi.org/10.1016/j.foodcont.2015.07.010.
P. Russo, M.P. Arena, D. Fiocco, et al., Lactobacillus plantarum with broad antifungal activity: a promising approach to increase safety and shelf-life of cereal-based products, Int. J. Food. Microbiol. 247 (2016) 48-54. https://doi.org/10.1016/j.ijfoodmicro.2016.04.027
S. Xu, T.D. Boylston, B.A. Glatz, Effect of inoculation level of Lactobacillus rhamnosus and yogurt cultures on conjugated linoleic acid content and quality attributes of fermented milk products, J. Food Sci. 71(4) (2010) C275-C280. https://doi.org/10.1111/j.1750-3841.2006.00010.x.
R.P.S. Oliveira, A.C.R. Florence, R.C. Silva, et al., Effect of different prebiotics on the fermentation kinetics, probiotic survival and fatty acids profiles in nonfat mixed-strain fermented milk, Int. J. Food Microbiol. 128(3) (2009) 467-472. https://doi.org/10.1016/j.ijfoodmicro.2008.10.012.
L. Ong, A. Henriksson, N.P. Shah, Development of probiotic Cheddar cheese containing Lactobacillus acidophilus, Lb. casei, Lb. paracasei and Bifidobacterium spp. and the influence of these bacteria on proteolytic patterns and production of organic acid, Int. Dairy J. 16(5) (2006) 446-456. https://doi.org/10.1016/j.idairyj.2005.05.008.
S.S. Chen, P. Cao, F.X. Lang, et al., Adhesion-related immunomodulatory activity of the screened Lactobacillus plantarum from Sichuan pickle, Curr. Microbiology 76 (2019) 29-36. https://doi.org/10.1007/s00284-018-1580-3.
B. Del Re, B. Sgorbati, M. Miglioli, et al., Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum, Lett. Appl. Microbiol. 31 (2000) 438-442. https://doi.org/10.1046/j.1365-2672.2000.00845.x.
E. Izquierdo, P. Horvatovich, E. Marchioni, et al., 2-DE and MS analysis of key proteins in the adhesion of Lactobacillus plantarum, a first step toward early selection of probiotics based on bacterial biomarkers, Electrophoresis 30 (2009) 949-956. https://doi.org/10.1002/elps.200800399.
J.Y. He, W.W. Wang, Z. Wu, et al., Effect of Lactobacillus reuteri on intestinal microbiota and immune parameters: involvement of sex differences, J. Funct. Foods 53 (2019) 36-43. https://doi.org/10.1016/j.jff.2018.12.010.
S. Trifonov, Y. Yamashita, M. Kase, et al., Overview and assessment of the histochemical methods and reagents for the detection of β-galactosidase activity in transgenic animals, Anat. Sci. Int. 91 (2016) 56–67. https://doi.org/10.1007/s12565-015-0300-3.
T. Vasiljevic, P. Jelen, Production of β-galactosidase for lactose hydrolysis in milk and dairy products using thermophilic lactic acid bacteria, Innov. Food Sci. Emerg. 2(2) (2001) 75-85. https://doi.org/10.1016/S1466-8564(01)00027-3.
S.N. Rajagopal, W.E. Sandine, Associative growth and proteolysis of Streptococcus thermophilus and Lactobacillus bulgaricus in skim milk, J. Dairy Sci. 73(4) (1990) 894-899. https://doi.org/10.3168/jds.S0022-0302(90)78745-0.
M. Carević, M. Vukašinović-Sekulić, S. Grbavčić, et al., Optimization of β-galactosidase production from lactic acid bacteria, Chem. Ind. 69(3) (2015) 305-312. https://doi.org/10.2298/HEMIND140303044C.
G.A. Somkuti, D.H. Steinberg, Pediocin production in milk by Pediococcus acidilactici in co-culture with Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus, J. Ind. Microbiol. Biot. 37 (2010) 65-69. https://doi.org/10.1007/s10295-009-0648-2.
C. Stanton, G. Gardiner, P.B. Lynch, et al., Probiotic cheese, Int. Dairy. J. 8(5-6) (1998) 491-496. https://doi.org/10.1016/S0958-6946(98)00080-6.
M.D. Iličić, S.D. Milanović, M. Carić, et al., Volatile compounds of functional dairy products, Acta Period Technol. 43 (2012) 11-19. https://doi.org/10.2298/APT1243011I.
M.E. Sanders, J. Hamilton, G. Reid, et al., A nonviable preparation of Lactobacillus acidophilus is not a probiotic, Clin. Infect. Dis. 44(6) (2007) 886-886. https://doi.org/10.1086/511694.
L. Trigueros, E. Sendra, Fatty acid and conjugated linoleic acid (CLA) content in fermented milks as assessed by direct methylation, LWT-Food Sci. Technol. 60(1) (2015) 315-319. https://doi.org/10.1016/j.lwt.2014.09.053.
M.R. Damin, E. Minowa, M.R. Alcântara, et al., Effect of cold storage on culture viability and some rheological properties of fermented milk prepared with yogurt and probiotic bacteria, J. Texture. Stud. 39(1) (2010) 40-55. https://doi.org/10.1111/j.1745-4603.2007.00129.x.
D.M.D.L. Alejandra, P. Gabriela, The application of probiotic fermented milks in cancer and intestinal inflammation, P. Nutr. Soc. 69(3) (2010) 421-428. https://doi.org/10.1017/S002966511000159X.
This work was supported by the National Natural Science Foundation of China [32072192, 31901668, 31671869], Key Research and Development Project of Zhejiang Province [2020C02042], the Natural Science Foundation of Zhejiang Province [LY19C200005], the Natural Science Foundation of Ningbo (202003N4129), the Open Project Program of the First-Class Bioengineering Disciplines in Zhejiang Province [KF2020007], the Graduate General Program of the Education Department in Zhejiang Province [Y202045625] and the K. C. Wong Magna Fund in Ningbo University.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).