Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice

Jiaping Pan; Huan Wang; Ye Li; Chenyang Lu

doi:10.1016/j.fshw.2021.11.005

Food Science and Human Wellness 2022, 11(2): 316-322 https://doi.org/10.1016/j.fshw.2021.11.005

Research Article |

Open Access | Issue | Published: 25 November 2021

Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice

Show Author's Information Hide Author's Information Jiaping Pan^{^a^,^b^,¹}, Huan Wang^{^a^,^b^,¹}, Ye Li^{^a}(

), Chenyang Lu^{^a}

School of Marine Science, Ningbo University, Ningbo 315832, China

Collage of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China

1Authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:

LC-PUFAs, Fish oil, Krill oil, Label-free quantitative proteomics, Lipids metabolism

Cite this article:

Pan J, Wang H, Li Y, et al. Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice. Food Science and Human Wellness, 2022, 11(2): 316-322. https://doi.org/10.1016/j.fshw.2021.11.005

Download citation

EndNote(RIS)

BibTeX

479

Views

Downloads

Citations

Crossref

WoS

Scopus

CSCD

Abstract Full text About this article

Abstract

Long-chain omega-3 polyunsaturated fatty acids (LC-PUFAs), known for having many health benefits, are usually present in three forms: triglycerides (TG), ethyl esters (EE), and phospholipid (PL). In this study, the effects of these three LC-PUFAs forms (fish oil for TG and EE, krill oil for PL) on the obese mice were compared, and the proteomic changes that focused on lipid metabolism were evaluated via label-free quantitative proteomics analysis. Compared with the model group, all three of the LC-PUFA form supplementations (labeled as the FO-TG group, FO-EE group and KO-PL groups) could significantly reduce body weight gain (P < 0.01). Low-density lipoprotein cholesterol levels were significantly decreased, whereas high-density lipoprotein cholesterol levels were significantly increased in the FO-TG group and FO-EE group (P < 0.01), and especially in the PL group (P < 0.001). Furthermore, proteomics analysis results suggested that some differentially expressed genes involved in the fatty acid degradation and oxidation pathways had a higher expression fold in the KO-PL group than in the FO-TG or FO-EE groups. Our results showed that dietary LC-PUFAs can reduce fat deposition and inhibit lipogenesis in the liver by upregulating the expression of proteins that are involved in the fatty acid degradation and oxidation pathways. Additionally, KO-PL elicits stronger effects than FO-TG or FO-EE.

Full text

Abstract

Full text

Outline

About this article

Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice

Show Author's information Hide Author's Information Jiaping Pan^{^a^,^b^,¹}, Huan Wang^{^a^,^b^,¹}, Ye Li^{^a}(

), Chenyang Lu^{^a}

School of Marine Science, Ningbo University, Ningbo 315832, China

Collage of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China

1Authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

Keywords: LC-PUFAs, Fish oil, Krill oil, Label-free quantitative proteomics, Lipids metabolism

References(22)

[1]

P. Flachs, M. Rossmeisl, M. Bryhn, et al., Cellular and molecular effects of n-3 polyunsaturated fatty acids on adipose tissue biology and metabolism, Clin. Sci. 116 (2009) 1-16. https://doi.org/10.1042/CS20070456.

DOI Google Scholar

[2]

J. Dyerberg, P.H. Madsen, J.M. Møller, et al., Bioavailability of marine n-3 fatty acid formulations, Prostaglandins, Leukotrienes Essent. Fatty Acids. 83 (2010) 137-141. https://doi.org/10.1016/j.plefa.2010.06.007.

DOI Google Scholar

[3]

J. Neubronner, J.P. Schuchardt, G. Kressel, et al., Enhanced increase of omega-3 index in response to long-term n-3 fatty acid supplementation from triacylglycerides versus ethyl esters, Eur. J. Clin. Nutr. 65 (2011) 247-254. https://10.1038/ejcn.2010.239.

DOI Google Scholar

[4]

J.C. Gigliotti, M.P. Davenport, S.K. Beamer, et al., Extraction and characterization of lipids from Antarctic krill (Euphausia superba), Food Chem. 125 (2011) 1028-1036. https://doi.org/10.1016/j.foodchem.2010.10.013.

DOI Google Scholar

[5]

J.P. Schuchardt, I. Schneider, H. Meyer, et al., Incorporation of EPA and DHA into plasma phospholipids in response to different omega-3 fatty acid formulations - a comparative bioavailability study of fish oil vs. krill oil, Lipids Health Dis. 10 (2011) 145. https://10.1186/1476-511X-10-145.

DOI Google Scholar

[6]

H. Che, J. Cui, M. Wen, et al., Long-term effects of docosahexaenoic acid-bound phospholipids and the combination of docosahexaenoic acid-bound triglyceride and egg yolk phospholipid on lipid metabolism in mice, J. Ocean Univ. China 17 (2018) 392-398. https://doi.org/10.1007/s11802-018-3444-7.

DOI Google Scholar

[7]

J.H. Song, Y. Inoue, T. Miyazawa, Oxidative stability of docosahexaenoic acid-containing oils in the form of phospholipids, triacylglycerols, and ethyl esters, Biosci. Biotechnol. Biochem. 61(12) (1997) 2085-2088. https://doi.org/10.1271/bbb.61.2085.

DOI Google Scholar

[8]

L.D. Lawson, B.G. Hughes, Human absorption of fish oil fatty acids as triacylglyceroils, free acids, or ethyl esters, Biochem. Biophys. Res. Commun. 152(1) (1988) 328-335. https://doi.org/10.1016/s0006-291x(88)80718-6.

DOI Google Scholar

[9]

K. Yurko-Mauro, J. Kralovec, E. Bailey-Hall, et al., Similar eicosapentaenoic acid and docosahexaenoic acid plams levels achieved with fish oil or krill oil in a randomized double-blind four-week bioavailability study, Lipids Health Dis. 14 (2015) 99. https://doi.org/10.1186/s12944-015-0109-z.

DOI Google Scholar

[10]

D.B. Jump, S.D. Clarke, A. Thelen, et al., Dietary polyunsaturated fatty acid regulation of gene transcription, Annu. Rev. Nutr. 14 (1994) 83-98. https://doi.org/10.1016/s0163-7827(96)00007-0.

DOI Google Scholar

[11]

I. Niot, H. Poirier, P.H. Besnard, Regulation of gene expression by fatty acids, special reference to fatty acid-binding protein (FABP), Biochimie. 79(2/3) (1997) 129-133. https://doi.org/10.1016/s0300-9084(97)81504-0.

DOI Google Scholar

[12]

X. Liu, J. Cui, Z. Li, et al., Comparative study of DHA-enriched phospholipids and EPA-enriched phospholipids on metabolic disorders in diet-induced-obese C57BL/6J mice, Eur. J. Lipid Sci. Technol. 116(3) (2014) 255-265. https://doi.org/10.1002/ejlt.201300407.

DOI Google Scholar

[13]

S.D. Clarke, D.B. Jump, Polyunsaturated fatty acid regulation of hepatic gene transcription, Lipids 31 (1996) 7-11. https://doi.org/10.1007/BF02637044.

DOI Google Scholar

[14]

S. Schmidt, J. Willers, F. Stahl, et al., Regulation of lipid metabolism-related gene expression in whole blood cells of normo-and dyslipodemic men after fish oil supplementation, Lipids Health Dis. 11 (2012) 172. https://doi.org/10.1186/1476-511X-11-172.

DOI Google Scholar

[15]

P. Flachs, O. Horakova, P. Brauner, et al., Polyunsaturated fatty acids of marine origin upregulate mitochondrial biogenensis and induce β-oxidation in white fat, Diabetologia. 48 (2005) 2365-2375. https://doi.org/10.1007/s00125-005-1944-7.

DOI Google Scholar

[16]

M. Schrader, J. Costello, L.F. Godinho, et al., Peroxisome-mitochondria interplay and disease, J. Inherited Metab. Dis. 38(4) (2015) 681-702. https://doi.org/10.1007/s10545-015-9819-7.

DOI Google Scholar

[17]

V. Ruipérez, F. Darios, B. Davletov, Alpha-synuclein, lipids and Parkinson's disease, Prog. Lipid Res. 49 (2010) 420-428. https://doi.org/10.1016/j.plipres.2010.05.004.

DOI Google Scholar

[18]

A.E. Leonard, S.L. Pereira, H. Sprecher, et al., Elongation of long-chain fatty acids, Prog. Lipid Res. 43(1) (2004) 36-54. https://doi.org/10/1016/S0163-782(03)00040-7.

[19]

D.C. Zeldin, Epoxygenase pathways of arachidonic acid metabolism, J. Biol. Chem. 276(39) (2001) 36059-36062. https://doi.org/10.1074/jbc.R100030200.

DOI Google Scholar

[20]

B. Staels, J. Dallongeville, J. Auwerx, et al., Mechanism of action of fibrates on lipid and lipoprotein metabolism, Circulation 98 (1998) 2088-2093. https://doi.org/10.1161/01.CIR.98.19.2088.

DOI Google Scholar

[21]

G.C. Shearer, O.V. Savinova, W.S. Harris, Fish oil - how does it reduce plasma triglycerides? Biochim. Biophys. Acta. 5 (2012) 843-851. https://doi.org/10.1016/j.bbalip.2011.10.011.

DOI Google Scholar

[22]

R. Schnitzer-Polokoff, D. Compton, G. Boykow, et al., Effects of acyl-CoA: cholesterol O-acyltransferase inhibition on cholesterol absorption and plasma lipoprotein composition in hamsters, Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 99(4) (1991) 665-670. https://doi.org/10.1016/0300-9629(91)90147-5.

DOI Google Scholar

About this article

Publication history

Acknowledgements

Rights and permissions

Publication history

Received: 22 April 2020

Revised: 12 October 2020

Accepted: 13 October 2020

Published: 25 November 2021

Issue date: March 2022

Copyright

Acknowledgements

This work was supported by the Regional Demonstration Project of Marine Economic Innovation and Development (2013 and 2016), National Natural Science Foundation of China (31800117), and the K. C. Wong Magna Fund offered by the Ningbo University.

Rights and permissions

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).