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Food Science and Human Wellness 2022, 11(1): 74-84 https://doi.org/10.1016/j.fshw.2021.07.009
Research Article | Open Access | Issue | Published: 11 September 2021

Mesona chinensis Benth polysaccharides alleviates liver injury by beneficial regulation of gut microbiota in cyclophosphamide-induced mice

Show Author's Information Yuzhen Hong,  Mingyue Shen,  Lixin Huang,  Ting Wu,  Jianhua Xie( )
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
Keywords:
Gut microbiota, Oxidative stress, Polysaccharide, Liver damage
Cite this article:
Hong Y, Shen M, Huang L, et al. Mesona chinensis Benth polysaccharides alleviates liver injury by beneficial regulation of gut microbiota in cyclophosphamide-induced mice. Food Science and Human Wellness, 2022, 11(1): 74-84. https://doi.org/10.1016/j.fshw.2021.07.009
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Abstract Full text About this article

There are a number of health benefits of Mesona chinensis Benth polysaccharide (MP), but little is known about its hepatoprotective effect and effect on gut microbiota composition in mice with liver damage induced by cyclophosphamide (CTX). This study indicated that MP supplementation effectively inhibited the production of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), enhanced liver antioxidant capacity and repaired liver damage in mice caused by CTX. The release of inflammatory cytokines in liver and the concentration of lipopolysaccharide (LPS) in serum were decreased, and the level of short chain fatty acids (SCFAs) in colon was increased after MP administration. Those effects may be correlated with the regulation of the gut microbiota. Importantly, MP restrained liver inflammatory responses induced by CTX may via increasing the SCFAs-producing bacteria family Ruminococcaceae and reduced LPS-producing bacteria genus Bacteroides. In short, the prevention of CTX-induced liver injury by supplementing MP is achieved at least in part by regulating the community structure of the gut microbiota, and MP is expected to be a potential prebiotic to treat and prevent liver diseases.


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Mesona chinensis Benth polysaccharides alleviates liver injury by beneficial regulation of gut microbiota in cyclophosphamide-induced mice

Show Author's information Yuzhen Hong, Mingyue Shen, Lixin Huang, Ting Wu, Jianhua Xie( )
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Abstract

There are a number of health benefits of Mesona chinensis Benth polysaccharide (MP), but little is known about its hepatoprotective effect and effect on gut microbiota composition in mice with liver damage induced by cyclophosphamide (CTX). This study indicated that MP supplementation effectively inhibited the production of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), enhanced liver antioxidant capacity and repaired liver damage in mice caused by CTX. The release of inflammatory cytokines in liver and the concentration of lipopolysaccharide (LPS) in serum were decreased, and the level of short chain fatty acids (SCFAs) in colon was increased after MP administration. Those effects may be correlated with the regulation of the gut microbiota. Importantly, MP restrained liver inflammatory responses induced by CTX may via increasing the SCFAs-producing bacteria family Ruminococcaceae and reduced LPS-producing bacteria genus Bacteroides. In short, the prevention of CTX-induced liver injury by supplementing MP is achieved at least in part by regulating the community structure of the gut microbiota, and MP is expected to be a potential prebiotic to treat and prevent liver diseases.

Keywords: Gut microbiota, Oxidative stress, Polysaccharide, Liver damage

References(45)

[1]

A. Emadi, R.J. Jones, R.A. Brodsky, Cyclophosphamide and cancer: golden annivers ary, Nat. Rev. Clin. Oncol. 6 (2009) 638-647. https://doi.org/10.1038/nrclinonc.2009.146.
DOI Google Scholar
[2]

P.D. King, M.C. Perry, Hepatotoxicity of chemotherapy, Oncologist 6 (2001) 162-176. https://doi.org/10.1634/theoncologist.6-2-162.
DOI Google Scholar
[3]

M.T. Madondo, M. Quinn, M. Plebanski, Low dose cyclophosphamide: mechanisms of T cell modulation, Cancer Treat Rev. 42 (2016) 3-9. https://doi.org/10.1016/j.ctrv.2015.11.005.
DOI Google Scholar
[4]

P. Vitaglione, F. Morisco, N. Caporaso, et al., Dietary antioxidant compounds and liver health, Crit. Rev. Food. Sci. Nutr. 44 (2004) 575-586. https://doi.org/10.1080/10408690490911701.
DOI Google Scholar
[5]

S.K. Asrani, H. Devarbhavi, J. Eaton, et al., Burden of liver diseases in the world, J. Hepatol. 70 (2019) 151-171. https://doi.org/10.1016/j.jhep.2018.09.014.
DOI Google Scholar
[6]

M.O. Germoush, Diosmin protects against cyclophosphamide-induced liver injury through attenuation of oxidative stress, inflammation and apoptosis, Int. J. Pharmacol. 12 (2016) 644-654. https://doi.org/10.3923/ijp.2016.644.654.
DOI Google Scholar
[7]

M. Zarei, T. Shivanandappa, Amelioration of cyclophosphamide-induced hepatotoxicity by the root extract of Decalepis hamiltonii in mice, Food. Chem. Toxicol. 57 (2013) 179-184. https://doi.org/10.1016/j.fct.2013.03.028.
DOI Google Scholar
[8]

Y. Yu, M.Y. Shen, Q.Q. Song, et al., Biological activities and pharmaceutical applications of polysaccharide from natural resources: a review, Carbohydr. Polym. 183 (2018) 91-101. https://doi.org/10.1016/j.carbpol.2017.12.009.
DOI Google Scholar
[9]

J.H. Xie, M.Y. Xie, S.P. Nie, et al., Isolation, chemical composition and antioxidant activities of a water-soluble polysaccharide from Cyclocarya paliurus (Batal.) Iljinskaja, Food. Chem. 119 (2010) 1626-1632. https://doi.org/10.1016/j.foodchem.2009.09.055.
DOI Google Scholar
[10]

J. Luo, C. Zhang, R. Liu, et al., Ganoderma lucidum polysaccharide alleviating colorectal cancer by alteration of special gut bacteria and regulation of gene expression of colonic epithelial cells, J. Funct. Foods. 47 (2018) 127-135. https://doi.org/10.1016/j.jff.2018.05.041.
DOI Google Scholar
[11]

X. Xu, J. Yang, Z. Ning, et al., Lentinula edodes-derived polysaccharide rejuvenates mice in terms of immune responses and gut microbiota, Food. Funct. 6 (2015) 2653-2663. https://doi.org/10.1039/C5FO00689A.
DOI Google Scholar
[12]

J. Xie, M. Jin, G.A. Morris, et al., Advances on bioactive polysaccharides from medicinal plants, Crit. Rev. Food. Sci. Nutr. 56(sup1) (2015) S60-S84. https://doi.org/10.1080/10408398.2015.1069255.
DOI Google Scholar
[13]

S. Lin, G. Hao, M. Long, et al., Oyster (Ostrea plicatula Gmelin) polysaccharides intervention ameliorates cyclophosphamide-Induced genotoxicity and hepatotoxicity in mice via the Nrf2-ARE pathway, Biomed. Pharmacother. 95 (2017) 1067-1071. https://doi.org/10.1016/j.biopha.2017.08.058.
DOI Google Scholar
[14]

L. Huang, M. Huang, M. Shen, et al., Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress, Int. J. Biol. Macromol. 136 (2019) 1000-1006. https://doi.org/10.1016/j.ijbiomac.2019.06.199.
DOI Google Scholar
[15]

L.H. Lin, J.H. Xie, S.C. Liu, et al., Polysaccharide from Mesona chinensis: extraction optimization, physicochemical characterizations and antioxidant activities, Int. J. Biol. Macromol. 99 (2017) 665-673. https://doi.org/10.1016/j.ijbiomac.2017.03.040.
DOI Google Scholar
[16]

L.X. Huang, M.Y. Shen, X.W. Zhang, et al., Effect of high-pressure microfluidization treatment on the physicochemical properties and antioxidant activities of polysaccharide from Mesona chinensis Benth, Carbohydr. Polym. 200 (2018) 191-199. https://doi.org/10.1016/j.carbpol.2018.07.087.
DOI Google Scholar
[17]

W. Wang, L. Jiang, Y. Ren, et al., Characterizations and hepatoprotective effect of polysaccharides from Mesona blumes against tetrachloride-induced acute liver injury in mice, Int. J. Biol. Macromol. 124 (2019) 788-795. https://doi.org/10.1016/j.ijbiomac.2018.11.260.
DOI Google Scholar
[18]

F. Backhed, R.E. Ley, J.L. Sonnenburg, et al., Host-bacterial mutualism in the human intestine, Science 307 (2005) 1915-1920. https://doi.org/10.1126/science.1104816.
DOI Google Scholar
[19]

G.A. Cresci, B. Glueck, M.R. McMullen, et al., Prophylactic tributyrin treatment mitigates chronic-binge ethanol-induced intestinal barrier and liver injury, J. Gastroenterol. Hepatol. 32 (2017) 1587-1597. https://doi.org/10.1111/jgh.13731.
DOI Google Scholar
[20]

P.A. van Leeuwen, M.A. Boermeester, A.P. Houdijk, et al., Clinical significance of translocation, Gut 35 (1994) S28-S34. https://doi.org/10.1136/gut.35.1_suppl.s28.
DOI Google Scholar
[21]

X.C. Peng, S.T. Li, J.M. Luo, et al., Effects of dietary fibers and their mixtures on short chain fatty acids and microbiota in mice guts, Food. Funct. 4 (2013) 932-938. https://doi.org/10.1039/c3fo60052a.
DOI Google Scholar
[22]

H.J. Flint, K.P. Scott, P. Louis, et al., The role of the gut microbiota in nutrition and health, Nat. Rev. Gastroenterol. Hepatol. 9 (2012) 577-589. https://doi.org/10.1038/nrgastro.2012.156.
DOI Google Scholar
[23]

R. Zhao, Q. Hu, G. Ma, et al., Effects of Flammulina velutipes polysaccharide on immune response and intestinal microbiota in mice, J. Funct. Foods. 56 (2019) 255-264. https://doi.org/10.1016/j.jff.2019.03.031.
DOI Google Scholar
[24]

Y. Xia, T. Wang, S. Yu, et al., Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism, Carbohydr. Polym. 207 (2019) 256-265. https://doi.org/10.1016/j.carbpol.2018.11.097.
DOI Google Scholar
[25]

J. Wu, H. Shao, J. Zhang, et al., Mussel polysaccharide alpha-D-glucan (MP-A) protects against non-alcoholic fatty liver disease via maintaining the homeostasis of gut microbiota and regulating related gut-liver axis signaling pathways, Int. J. Biol. Macromol. 130 (2019) 68-78. https://doi.org/10.1016/j.ijbiomac.2019.02.097.
DOI Google Scholar
[26]

D. Hou, Q. Zhao, L. Yousaf, et al., Consumption of mung bean (Vigna radiata L.) attenuates obesity, ameliorates lipid metabolic disorders and modifies the gut microbiota composition in mice fed a high-fat diet, J. Funct. Foods. 64 (2020) 1756-4646. https://doi.org/10.1016/j.jff.2019.103687.
DOI Google Scholar
[27]

S.R. Fahmy, A.I. Amien, F.M. Abd-Elgleel, et al., Antihepatotoxic efficacy of Mangifera indica L. polysaccharides against cyclophosphamide in rats, Chem. Biol. Interact. 244 (2016) 113-120. https://doi.org/10.1016/j.cbi.2015.11.009.
DOI Google Scholar
[28]

Y.V. Yuan, D.E. Bone, M.F. Carrington, Antioxidant activity of dulse (Palmaria palmata) extract evaluated in vitro, Food. Chem. 91 (2015) 485-494. https://doi.org/10.1016/j.foodchem.2004.04.039.
DOI Google Scholar
[29]

D.H. Mak, S.P. Ip, P.C. Li, et al., Alterations in tissue glutathione antioxidant system in streptozotocin-induced diabetic rats, Mol. Cell. Biochem. 162 (1996) 153-158. https://doi.org/10.1007/BF00227543.
DOI Google Scholar
[30]

R.K. Nitharwal, H. Patel, M.S. Karchuli, et al., Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity, Indian. J. Pharmacol. 45 (2013) 502-507. https://doi.org/10.4103/0253-7613.117783.
DOI Google Scholar
[31]

G. Malaguarnera, M. Giordano, G. Nunnari, et al., Gut microbiota in alcoholic liver disease: pathogenetic role and therapeutic perspectives, World. J. Gastroenterol. 20 (2014) 16639-16648. https://doi.org/10.3748/wjg.v20.i44.16639.
DOI Google Scholar
[32]

A. Schwiertz, D. Taras, K. Schafer, et al., Microbiota and SCFA in lean and overweight healthy subjects, Obesity (Silver Spring) 18 (2010) 190-195. https://doi.org/10.1038/oby.2009.167.
DOI Google Scholar
[33]

J.S. Park, E.J. Lee, J.C. Lee, et al., Anti-inflammatory effects of short chain fatty acids in IFN-gamma-stimulated RAW 264.7 murine macrophage cells: involvement of NF-kappaB and ERK signaling pathways, Int. Immunopharmacol. 7 (2007) 70-77. https://doi.org/10.1016/j.intimp.2006.08.015.
DOI Google Scholar
[34]

X. Xu, P. Xu, C. Ma, et al., Gut microbiota, host health, and polysaccharides, Biotechnol. Adv. 31 (2013) 318-337.https://doi.org/10.1016/j.biotechadv.2012.12.009.
DOI Google Scholar
[35]

J. Hu, S. Nie, C. Li, et al., In vitro fermentation of polysaccharide from the seeds of Plantago asiatica L. by human fecal microbiota, Food. Hydrocoll. 33 (2013) 384-392. https://doi.org/10.1016/j.foodhyd.2013.04.006.
DOI Google Scholar
[36]

I. Sekirov, S.L. Russell, L.C. Antunes, et al., Gut microbiota in health and disease, Physiol. Rev. 90 (2010) 859-904. https://doi.org/10.1152/physrev.00045.2009.
DOI Google Scholar
[37]

M.K. Takanori Kana, The gut microbiota and inflammatory bowel disease, Semin. Immunopathol. 37 (2015) 47-55. https://doi.org/10.1007/s00281-014-0454-4.
DOI Google Scholar
[38]

M. Lyu, Y.F. Wang, G.W. Fan, et al., Balancing herbal medicine and functional food for prevention and treatment of cardiometabolic diseases through modulating gut microbiota, Front. Microbiol. 8 (2017) 2146. https://doi.org/10.3389/fmicb.2017.02146.
DOI Google Scholar
[39]

Q. Shang, X. Shan, C. Cai, et al., Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae, Food. Funct. 7 (2016) 3224-3232. https://doi.org/10.1039/C6FO00309E.
DOI Google Scholar
[40]

C.J. Zheng, R. Liu, B. Xue, et al., Impact and consequences of polyphenols and fructooligosaccharide interplay on gut microbiota in rats, Food. Funct. 8 (2017) 1925-1932. https://doi.org/10.1039/C6FO01783E.
DOI Google Scholar
[41]

H. Wang, Y. Li, X. Feng, et al., Dysfunctional gut microbiota and relative co-abundance network in infantile eczema, Gut Pathog. 8 (2016) 36. https://doi.org/10.1186/s13099-016-0118-0.
DOI Google Scholar
[42]

F.F. Anhe, R.T. Nachbar, T.V. Varin, et al., Treatment with camu camu (Myrciaria dubia) prevents obesity by altering the gut microbiota and increasing energy expenditure in diet-induced obese mice, Gut 68 (2019) 453-464. https://doi.org/10.1136/gutjnl-2017-315565.
DOI Google Scholar
[43]

D.L. Chen, C.Q. Zheng, J. Yang, et al., Immunomodulatory activities of a fungal protein extracted from Hericium erinaceus through regulating the gut microbiota, Front. Immunol. 8 (2017) 666. https://doi.org/10.3389/fimmu.2017.00666.
DOI Google Scholar
[44]

L. Zhang, Y.N. Wu, T. Chen, et al., Relationship between intestinal microbial dysbiosis and primary liver cancer, Hepatobiliary. Pancreat. Dis. Int. 18 (2019) 149-157. https://doi.org/10.1016/j.hbpd.2019.01.002.
DOI Google Scholar
[45]

T. Liu, J. Li, Y. Liu, et al., Short-chain fatty acids suppress lipopolysaccharide-induced production of nitric oxide and proinflammatory cytokines through inhibition of NF-kappaB pathway in RAW264.7 cells, Inflammation 35 (2012) 1676-1684. https://doi.org/10.1007/s10753-012-9484-z.
DOI Google Scholar
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Publication history

Received: 31 March 2020
Revised: 05 June 2020
Accepted: 10 June 2020
Published: 11 September 2021
Issue date: January 2022

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© 2021 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgement

This study was supported by grants from the National Natural Science Foundation of China (21566024), and the Natural Science Foundation of Jiangxi province, China (20181ACB20013).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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