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Food Science and Human Wellness 2021, 10(4): 536-544 https://doi.org/10.1016/j.fshw.2021.04.016
Research Article | Open Access | Issue | Published: 04 June 2021

Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia

Show Author's Information Yingyin Xua,#,  Yuanhui Lib,#,  Yuxiao Luc,  Xiaobin Fengb,  Guoting Tiand,  Qinghong Liub( )
Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
Department of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, China
Department of Environment and Chemical Engineering, Tangshan College, Tangshan 063000, China
Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China

# The authors contribute equally.Peer review under responsibility of KeAi Communications Co., Ltd]]>

Keywords:
Antioxidant activity, Polysaccharide, Lepista sordida, Hepatoprotective effects
Cite this article:
Xu Y, Li Y, Lu Y, et al. Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia. Food Science and Human Wellness, 2021, 10(4): 536-544. https://doi.org/10.1016/j.fshw.2021.04.016
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Abstract Full text About this article

A novel alkali-soluble polysaccharide from Lepista sordida (LSAP) mycelia with antioxidative and hepatoprotective activities was characterized. The weight-average molecular weight and number-average molecular weight of LSAP were 1.442×103 and 6.05×102 kDa, respectively. LSAP was consisted of glucose (57.9%), xylose (31.8%), and small amounts of rhamnose, arabinose, galactose, glucuronic acid, and galacturonic acid (1.2%–3.1%). The FT-IR and 2D NMR confirmed that LSAP was composed of Xylp, Araf, 4-O-Me-α-D-GlcpA, (1→4)-linked β-D-Glcp, and (1→4)-α-D-GalA, and β-glycosidic linkages between these sugar units. LSAP displayed notable effects on 1, 1-dephenyl-2-picryhydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, lipid peroxidation inhibitory ability, reducing power and Fe2+ chelating property. These biological effects were further verified by suppressing CCl4-induced oxidative liver damage in mice at doses of 100 and 200 mg/kg. Administration of LSAP in mice prior to CCl4 significantly prevented the CCl4-induced elevation in serum alanine aminotransferase, aspartate aminotransferase, and hepatic malondialdehyde. Mice treated with LSAP demonstrated to increase activities in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver. We also found out that LSAP prevented CCl4-induced oxidative liver histological alteration. LSAP may exert hepatoprotective effects against CCl4-induced damage through antioxidant mechanisms in model mice.


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About this article

Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia

Show Author's information Yingyin Xua,#, Yuanhui Lib,#, Yuxiao Luc, Xiaobin Fengb, Guoting Tiand, Qinghong Liub( )
Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
Department of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, China
Department of Environment and Chemical Engineering, Tangshan College, Tangshan 063000, China
Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China

# The authors contribute equally.Peer review under responsibility of KeAi Communications Co., Ltd]]>

Abstract

A novel alkali-soluble polysaccharide from Lepista sordida (LSAP) mycelia with antioxidative and hepatoprotective activities was characterized. The weight-average molecular weight and number-average molecular weight of LSAP were 1.442×103 and 6.05×102 kDa, respectively. LSAP was consisted of glucose (57.9%), xylose (31.8%), and small amounts of rhamnose, arabinose, galactose, glucuronic acid, and galacturonic acid (1.2%–3.1%). The FT-IR and 2D NMR confirmed that LSAP was composed of Xylp, Araf, 4-O-Me-α-D-GlcpA, (1→4)-linked β-D-Glcp, and (1→4)-α-D-GalA, and β-glycosidic linkages between these sugar units. LSAP displayed notable effects on 1, 1-dephenyl-2-picryhydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, lipid peroxidation inhibitory ability, reducing power and Fe2+ chelating property. These biological effects were further verified by suppressing CCl4-induced oxidative liver damage in mice at doses of 100 and 200 mg/kg. Administration of LSAP in mice prior to CCl4 significantly prevented the CCl4-induced elevation in serum alanine aminotransferase, aspartate aminotransferase, and hepatic malondialdehyde. Mice treated with LSAP demonstrated to increase activities in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver. We also found out that LSAP prevented CCl4-induced oxidative liver histological alteration. LSAP may exert hepatoprotective effects against CCl4-induced damage through antioxidant mechanisms in model mice.

Keywords: Antioxidant activity, Polysaccharide, Lepista sordida, Hepatoprotective effects

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Publication history

Received: 14 October 2020
Revised: 06 November 2020
Accepted: 09 November 2020
Published: 04 June 2021
Issue date: July 2021

Copyright

© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

This study was supported by grants from the National Key R & D Program of China (Project No. 2018YFD0400200) and the China Agriculture Research System (No. CARS-20-08B).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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