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Food Science and Human Wellness 2021, 10(4): 514-522 https://doi.org/10.1016/j.fshw.2021.04.014
Research Article | Open Access | Issue | Published: 04 June 2021

Therapeutic effect of natural melanin from edible fungus Auricularia auricula on alcohol-induced liver damage in vitro and in vivo

Show Author's Information Ruolin Houa,b,  Xin Liua,b( ),  Xiaoping Wuc,  Mingfeng Zhengb,  Junsheng Fud( )
The Third People's Hospital Health Care Group of Cixi, Ningbo 315000, China
College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Peer review under responsibility of KeAi Communications Co., Ltd

Keywords:
Liver damage, Auricularia auricula, Edible fungus, Melanin, Therapeutic
Cite this article:
Hou R, Liu X, Wu X, et al. Therapeutic effect of natural melanin from edible fungus Auricularia auricula on alcohol-induced liver damage in vitro and in vivo. Food Science and Human Wellness, 2021, 10(4): 514-522. https://doi.org/10.1016/j.fshw.2021.04.014
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Abstract Full text About this article

This study explored the therapeutic effects of Auricularia auricula melanin (AAM) on alcoholic liver damage in vitro and in vivo. Human normal liver L02 cells were pre-treated with ethanol and then treated with AAM to explore the therapeutic effect of AAM on ethanol-induced hepatocyte injury. The results show that AAM significantly elevated the cell viability, ameliorated the cell morphology, reduced the ROS and increased the GSH/GSSG of ethanol-pretreated L02 cells. Then, mice were administered with ethanol to induce acute alcoholic liver damage, and administered with AAM to further study the therapeutic effect of AAM on alcoholic liver damage in mice. As a result, AAM reduced the levels of ALT, AST, TG, and MDA, increased the levels of ADH, SOD, and CAT in liver damage mice. The therapeutic effect of AAM may be related to inhibition of CYP2E1 expression and activation of Nrf2 and its downstream antioxidase. The research enriched the bioactivity of AAM and provided some ideas for the development of melanin-related health foods.


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About this article

Therapeutic effect of natural melanin from edible fungus Auricularia auricula on alcohol-induced liver damage in vitro and in vivo

Show Author's information Ruolin Houa,b, Xin Liua,b( ), Xiaoping Wuc, Mingfeng Zhengb, Junsheng Fud( )
The Third People's Hospital Health Care Group of Cixi, Ningbo 315000, China
College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Peer review under responsibility of KeAi Communications Co., Ltd

Abstract

This study explored the therapeutic effects of Auricularia auricula melanin (AAM) on alcoholic liver damage in vitro and in vivo. Human normal liver L02 cells were pre-treated with ethanol and then treated with AAM to explore the therapeutic effect of AAM on ethanol-induced hepatocyte injury. The results show that AAM significantly elevated the cell viability, ameliorated the cell morphology, reduced the ROS and increased the GSH/GSSG of ethanol-pretreated L02 cells. Then, mice were administered with ethanol to induce acute alcoholic liver damage, and administered with AAM to further study the therapeutic effect of AAM on alcoholic liver damage in mice. As a result, AAM reduced the levels of ALT, AST, TG, and MDA, increased the levels of ADH, SOD, and CAT in liver damage mice. The therapeutic effect of AAM may be related to inhibition of CYP2E1 expression and activation of Nrf2 and its downstream antioxidase. The research enriched the bioactivity of AAM and provided some ideas for the development of melanin-related health foods.

Keywords: Liver damage, Auricularia auricula, Edible fungus, Melanin, Therapeutic

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Publication history

Received: 26 September 2020
Revised: 16 December 2020
Accepted: 20 December 2020
Published: 04 June 2021
Issue date: July 2021

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© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

This work was financially supported by the special fund project for technological innovation of Fujian Agriculture and Forestry University (CXZX2019055G).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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