Journal Home > Volume 10 , Issue 4
Food Science and Human Wellness 2021, 10(4): 490-496 https://doi.org/10.1016/j.fshw.2021.04.011
Research Article | Open Access | Issue | Published: 04 June 2021

Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice

Show Author's Information Kudrat Randhawaa,  Varinder Singhb( ),  Sanimardeep Kaura,  Ravinder Kaura,  Suresh Kumara,  Richa Shria( )
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India
Chitkara College of Pharmacy, Chitkara University, Punjab, India

Peer review under responsibility of KeAi Communications Co., Ltd

Keywords:
Alzheimer's disease, Mushrooms, Resveratrol, Acetylcholinesterase inhibitor, Pleurotus florida
Cite this article:
Randhawa K, Singh V, Kaur S, et al. Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice. Food Science and Human Wellness, 2021, 10(4): 490-496. https://doi.org/10.1016/j.fshw.2021.04.011
Download citation
EndNote(RIS)
BibTeX

310

Views

30

Downloads

Citations

12

Crossref

10

WoS

10

Scopus

0

CSCD

Abstract Full text About this article

Our earlier work showed that hydromethanol extract (HME) of Pleurotus florida had antioxidant and anti-cholinesterase potential. This study aimed at isolating the constituent responsible for the activities. HME was subjected to bioactivity guided fractionation using in vitro anti-acetylcholinesterase (Ellman method) and antioxidant (DPPH scavenging assay). The most active constituent was evaluated in vivo employing i.c.v. streptozotocin (STZ) induced dementia in mice. Morris water maze test was used for evaluating memory, followed by biochemical estimations and histopathological studies. Bioactivity guided fractionation resulted in isolation of resveratrol (identified by IR, NMR and MS) and its content in P. florida fruiting bodies was 0.0098% (m/m, by a validated TLC densitometric method). It improved STZ induced dementia and neurodegeneration in mice by reducing brain acetylcholinesterase action and oxidative stress. The observed effects might be the presence of resveratrol. Our results further endorse that P. florida derived resveratrol can be a promising therapy for Alzheimer's disease.


menu
Abstract
Full text
Outline
About this article

Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice

Show Author's information Kudrat Randhawaa, Varinder Singhb( ), Sanimardeep Kaura, Ravinder Kaura, Suresh Kumara, Richa Shria( )
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India
Chitkara College of Pharmacy, Chitkara University, Punjab, India

Peer review under responsibility of KeAi Communications Co., Ltd

Abstract

Our earlier work showed that hydromethanol extract (HME) of Pleurotus florida had antioxidant and anti-cholinesterase potential. This study aimed at isolating the constituent responsible for the activities. HME was subjected to bioactivity guided fractionation using in vitro anti-acetylcholinesterase (Ellman method) and antioxidant (DPPH scavenging assay). The most active constituent was evaluated in vivo employing i.c.v. streptozotocin (STZ) induced dementia in mice. Morris water maze test was used for evaluating memory, followed by biochemical estimations and histopathological studies. Bioactivity guided fractionation resulted in isolation of resveratrol (identified by IR, NMR and MS) and its content in P. florida fruiting bodies was 0.0098% (m/m, by a validated TLC densitometric method). It improved STZ induced dementia and neurodegeneration in mice by reducing brain acetylcholinesterase action and oxidative stress. The observed effects might be the presence of resveratrol. Our results further endorse that P. florida derived resveratrol can be a promising therapy for Alzheimer's disease.

Keywords: Alzheimer's disease, Mushrooms, Resveratrol, Acetylcholinesterase inhibitor, Pleurotus florida

References(38)

[1]

J.M. Long, D.M. Holtzman, Alzheimer disease: an update on pathobiology and treatment strategies, Cell 179 (2019) 312-339. http://doi.org/10.1016/j.cell.2019.09.001.
DOI Google Scholar
[2]

D. Bishara, J. Sauer, D. Taylor, The pharmacological management of Alzheimer's disease, Prog. Neurol. Psychiatry 19 (2015) 9-16. http://doi.org/10.1002/pnp.151.
DOI Google Scholar
[3]

T. Singh, N. Bagga, A. Kaur, et al., Agmatine for combined treatment of epilepsy, depression and cognitive impairment in chronic epileptic animals, Biomed. Pharmacother. 92 (2017) 720-725. http://doi.org/10.1016/j.biopha.2017.05.085.
DOI Google Scholar
[4]

R. Kaur, V. Singh, R. Shri, Anti-amnesic effects of Ganoderma species: a possible cholinergic and antioxidant mechanism, Biomed. Pharmacother. 92 (2017) 1055-1061. http://doi.org/10.1016/j.biopha.2017.06.029.
DOI Google Scholar
[5]

K. Randhawa, R. Shri, Mushrooms in the management of Alzheimer's: a review, J. Pharm. Biol. 5 (2015) 278-288.
Google Scholar
[6]

C.W. Phan, P. David, V. Sabaratnam, Edible and medicinal mushrooms: emerging brain food for the mitigation of neurodegenerative diseases, J. Med. Food 20 (2017) 1-10. http://doi.org/10.1089/jmf.2016.3740.
DOI Google Scholar
[7]

A. Barh, V.P. Sharma, S.K. Annepu, et al., Genetic improvement in Pleurotus (oyster mushroom): a review, 3 Biotech 9 (2019) 322. http://doi.org/10.1007/s13205-019-1854-x.
DOI Google Scholar
[8]

G. Guzmán, Genus Pleurotus (Jacq. : Fr. ) P. Kumm. (Agaricomycetideae): diversity, taxonomic problems, and cultural and traditional medicinal uses, Int. J. Med. Mushrooms 2 (2000) 95-123. http://doi.org/10.1615/IntJMedMushr.v2.i2.10.
DOI Google Scholar
[9]

R.C. Corrêa, T. Brugnari, A. Bracht, et al., Biotechnological, nutritional and therapeutic uses of Pleurotus spp. (oyster mushroom) related with its chemical composition: a review on the past decade findings, Trends Food Sci. Technol. 50 (2016) 103-117. http://doi.org/10.1016/j.tifs.2016.01.012.
DOI Google Scholar
[10]

G. Tel, M. Ozturk, M.E. Duru, et al., Antioxidant and anticholinesterase activities of five wild mushroom species with total bioactive contents, Pharm. Biol. 53 (2015) 824-830. http://doi.org/10.3109/13880209.2014.943245.
DOI Google Scholar
[11]

T.K. Nguyen, K.H. Im, J. Choi, et al., Evaluation of antioxidant, anti-cholinesterase, and anti-inflammatory effects of culinary mushroom Pleurotus pulmonarius, Mycobiology 44 (2016) 291-301. http://doi.org/10.5941/MYCO.2016.44.4.291.
DOI Google Scholar
[12]

K. Randhawa, R. Shri, Comparison of antioxidant and anticholinesterase activities of selected Pleurotus species (Agaricomycetes) from India, Int. J. Med. Mushrooms 20 (2018) 739-748. http://doi.org/10.1615/IntJMedMushrooms.2018027033.
DOI Google Scholar
[13]

G.L. Ellman, K.D. Courtney, V. Andres, et al., A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem. Pharmacol. 7 (1961) 88-95. http://doi.org/10.1016/0006-2952(61)90145-9.
DOI Google Scholar
[14]

V. Singh, A. Kahol, I.P. Singh, et al., Evaluation of anti-amnesic effect of extracts of selected Ocimum species using in-vitro and in-vivo models, J. Ethnopharmacol. 193 (2016) 490-499. http://doi.org/10.1016/j.jep.2016.10.026.
DOI Google Scholar
[15]

M.S. Blois, Antioxidant determinations by the use of a stable free radical, Nature 181 (1958) 1199-1200. http://doi.org/10.1038/1811199a0.
DOI Google Scholar
[16]
ICH Guideline. Validation of analytical procedures: text and methodology Q2 (R1), In International conference on harmonization, Geneva, Switzerland (2005) 11-12.
[17]

A. Kaur, K. Randhawa, V. Singh, et al., Bioactivity-guided isolation of acetylcholinesterase inhibitor from Ganoderma mediosinense (Agaricomycetes), Int. J. Med. Mushrooms 21(8) (2019) 755-763. http://doi.org/10.1615/IntJMedMushrooms.2019031508.
DOI Google Scholar
[18]

A. Kumar, N. Singh, Pharmacological activation of protein kinase A improves memory loss and neuropathological changes in a mouse model of dementia of Alzheimer's type, Behav. Pharmacol. 28 (2017) 187-198. http://doi.org/10.1097/fbp.0000000000000294.
DOI Google Scholar
[19]

R. Morris, Developments of a water-maze procedure for studying spatial learning in the rat, J. Neurosci. Methods 11 (1984) 47-60. http://doi.org/10.1016/0165-0270(84)90007-4.
DOI Google Scholar
[20]

V. Singh, P. Krishan, R. Shri, Amelioration of ischaemia reperfusioninduced cerebral injury in mice by liposomes containing Allium cepa fraction administered intranasally, Artif. Cells Nanomed. Biotechnol. 46 (2018) S982-S992. http://doi.org/10.1080/21691401.2018.1523181.
DOI Google Scholar
[21]

O.H. Lowry, N.J. Rosebrough, A.L. Farr, et al., Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275. http://doi.org/10.1515/bchm2.1951.286.1-6.270.
DOI Google Scholar
[22]

H. Ohkawa, Assay of lipid peroxidation in animal tissues by thiobarbituric acid reaction, Anal. Biochem. 95 (1979) 371-379. http://doi.org/10.1016/0003-2697(79)90738-3.
DOI Google Scholar
[23]

E. Beutler, Improved method for the determination of blood glutathione, J. Lab. Clin. Med. 61 (1963) 882-888. http://doi.org/10.1057/jibs.2013.3.
DOI Google Scholar
[24]

V. Singh, P. Krishan, N. Singh, et al., Amelioration of ischemiareperfusion induced functional and biochemical deficit in mice by Ocimum kilimandscharicum leaf extract, Biomed. Pharmacother. 85 (2017) 556-563. http://doi.org/10.1016/j.biopha.2016.11.064.
DOI Google Scholar
[25]

T. Singh, R.K. Goel, Neuroprotective effect of Allium cepa L. in aluminium chloride induced neurotoxicity, Neurotoxicology 49 (2015) 1-7. http://doi.org/10.1016/j.neuro.2015.04.007.
DOI Google Scholar
[26]

A.S. Banchroft, D.R. Turner, Theory and practice of histological techniques (4th edn). (1996) China: Elsevier Health Sciences.
[27]

P. Grieb, Intracerebroventricular streptozotocin injections as a model of Alzheimer's disease: in search of a relevant mechanism, Mol. Neurobiol. 53 (2016) 1741-1752. http://doi.org/10.1007/s12035-015-9132-3.
DOI Google Scholar
[28]

M.G. Fronza, R. Baldinotti, M.C. Martins, et al., Rational design, cognition and neuropathology evaluation of QTC-4-MeOBnE in a streptozotocin-induced mouse model of sporadic Alzheimer's disease, Sci. Rep. 9 (2019) 1-4.
DOI Google Scholar
[29]

C.V.Vorhees, M.T.Williams, Morris water maze: procedures for assessing spatial and related forms of learning and memory, Nat.Protoc.1 (2006) 848.http://doi.org/10.1038/nprot.2006.116.
DOI Google Scholar
[30]

P.Barai, N.Raval, S.Acharya, et al., Bergenia ciliata ameliorates streptozotocin-induced spatial memory deficits through dual cholinesterase inhibition and attenuation of oxidative stress in rats, Biomed.Pharmacother.102 (2018) 966-980.http://doi.org/10.1016/j.biopha.2018.03.115.
DOI Google Scholar
[31]

S.Sasaki-Hamada, M.Ikeda, J.I.Oka, Glucagon-like peptide-2 rescues memory impairments and neuropathological changes in a mouse model of dementia induced by the intracerebroventricular administration of streptozotocin, Sci.Rep.9 (2018) 1-8.http://doi.org/10.1038/s41598-019-50167-3.
DOI Google Scholar
[32]

C.Wang, Z.Cai, W.Wang, et al., Piperine attenuates cognitive impairment in an experimental mouse model of sporadic Alzheimer's disease, J.Nutr.Biochem.70 (2019) 147-155.http://doi.org/ 10.1016/j.jnutbio.2019.05.009.
DOI Google Scholar
[33]

C.Sanchez, Reactive oxygen species and antioxidant properties from mushrooms, Synth.Syst.Biotechnol.2 (2017) 13-22.http://doi.org/10.1016/j.synbio.2016.12.001.
DOI Google Scholar
[34]

I.C.Ferreira, L.Barros, R.M.Abreu, Antioxidants in wild mushrooms, Curr.Med.Chem.16 (2009) 1543-1560.http://doi.org/10.2174/092986709787909587.
DOI Google Scholar
[35]

L.Kang, Q.Li, J.Lin, et al., Biosynthesis of resveratrol in blastospore of the macrofungus Tremella fuciformis, Mol.Biotechnol.57 (2015) 675-684.http://doi.org/10.1007/s12033-015-9858-1.
DOI Google Scholar
[36]

C.Halls, O.Yu, Potential for metabolic engineering of resveratrol biosynthesis, Trends Biotechnol.26 (2008) 77-81.http://doi.org/10.1016/j.tibtech.2007.11.002.
DOI Google Scholar
[37]

M.Sharma, Y.K.Gupta, Chronic treatment with trans resveratrol prevents intracerebroventricular streptozotocin induced cognitive impairment and oxidative stress in rats, Life Sci.71 (2002) 2489-2498.http://doi.org/10.1016/S0024-3205(02)02083-0.
DOI Google Scholar
[38]

T.Ahmed, S.Javed, S.Javed, et al., Resveratrol and Alzheimer's disease: mechanistic insights, Mol.Neurobiol.54 (2017) 2622-2635.http://doi.org/10.1007/s12035-016-9839-9.
DOI Google Scholar
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 09 September 2020
Revised: 10 October 2020
Accepted: 12 October 2020
Published: 04 June 2021
Issue date: July 2021

Copyright

© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgements

The study was funded by University Grants Commission under UGC BSR fellowship scheme (Award no. F.4.25-1/2013-14(BSR)/7-265/2009(BSR)). The authors are thankful to Prof. Nirmal Singh and Mr. Pankaj Bhatia, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India for their help in carrying out animal studies. The authors are also thankful to DBT-IPLS project (Grant no: BT/PR4548/INF/22/146/2012), Punjabi University, Patiala, Punjab, India for providing HPTLC facility and Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India for providing the necessary laboratory facilities for this work.

Rights and permissions

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Return