Journal Home > Volume 10 , Issue 4
Food Science and Human Wellness 2021, 10(4): 480-489 https://doi.org/10.1016/j.fshw.2021.04.010
Research Article | Open Access | Issue | Published: 04 June 2021

The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics

Show Author's Information Jinbin Hao1,  Liyun Ye1,  Guoliang Meng,  Yanjiao Song,  Junsheng Fu,  Xiaoping Wu( )
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, China

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd

Keywords:
Transcriptome, Polysaccharide, Trametes lactinea, Hepatoprotective, Metabolome
Cite this article:
Hao J, Ye L, Meng G, et al. The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics. Food Science and Human Wellness, 2021, 10(4): 480-489. https://doi.org/10.1016/j.fshw.2021.04.010
Download citation
EndNote(RIS)
BibTeX

338

Views

43

Downloads

Citations

7

Crossref

7

WoS

8

Scopus

2

CSCD

Abstract Full text About this article

Trametes lactinea mycelia polysaccharides (TLMPS) have a wide range of bioactivities. The potential mechanisms of action of TLMPS against acute alcohol-induced liver injury in vivo were investigated by analyzing the physical and chemical properties of TLMPS and its protective effects on a mouse model of alcoholic liver injury. TLMPS protected the liver against alcohol-induced injury, as evidenced by the reduced alcohol-induced elevation of the liver index, serum biochemical indices, and maintenance of hepatic morphology. Potential mechanisms were analyzed using transcriptome and metabolome analyses. The transcriptome data revealed the involvement of many differentially expressed genes in chemical carcinogenesis, drug metabolism, and metabolism of xenobiotics by cytochrome P450. The metabolome analysis revealed that TLMPS significantly regulated specific metabolites in the liver, including organic acids, lipids, nucleosides, and organic oxygen compounds. KEGG enrichment analysis revealed the significant involvement of different metabolites in choline metabolism, ATP-binding cassette transporters, and glycerophospholipid metabolism. Assessment of the changes in gene expression and metabolites revealed the significantly different expression of several genes encoding key enzymes and metabolites in choline metabolism pathway. The collective findings confirmed that choline metabolism plays an important role in the protective effects of TLMPS against acute alcoholic liver injury.


menu
Abstract
Full text
Outline
About this article

The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics

Show Author's information Jinbin Hao1, Liyun Ye1, Guoliang Meng, Yanjiao Song, Junsheng Fu, Xiaoping Wu( )
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, China

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd

Abstract

Trametes lactinea mycelia polysaccharides (TLMPS) have a wide range of bioactivities. The potential mechanisms of action of TLMPS against acute alcohol-induced liver injury in vivo were investigated by analyzing the physical and chemical properties of TLMPS and its protective effects on a mouse model of alcoholic liver injury. TLMPS protected the liver against alcohol-induced injury, as evidenced by the reduced alcohol-induced elevation of the liver index, serum biochemical indices, and maintenance of hepatic morphology. Potential mechanisms were analyzed using transcriptome and metabolome analyses. The transcriptome data revealed the involvement of many differentially expressed genes in chemical carcinogenesis, drug metabolism, and metabolism of xenobiotics by cytochrome P450. The metabolome analysis revealed that TLMPS significantly regulated specific metabolites in the liver, including organic acids, lipids, nucleosides, and organic oxygen compounds. KEGG enrichment analysis revealed the significant involvement of different metabolites in choline metabolism, ATP-binding cassette transporters, and glycerophospholipid metabolism. Assessment of the changes in gene expression and metabolites revealed the significantly different expression of several genes encoding key enzymes and metabolites in choline metabolism pathway. The collective findings confirmed that choline metabolism plays an important role in the protective effects of TLMPS against acute alcoholic liver injury.

Keywords: Transcriptome, Polysaccharide, Trametes lactinea, Hepatoprotective, Metabolome

References(45)

[1]

A.Y. Yus, M.D. Mashitah, S.Y. Ahmad, The effect of culture conditions on the growth of T. lactinea and anti-inflammatory activities via in vitro inhibition of hyaluronidase and lipoxygenase enzyme activities, J. Taiwan Inst. Chem. E. 45 (2014) 2054-2059. https://doi:10.1016/j.jtice.2014.04.003.
DOI Google Scholar
[2]

Q.Y. Zhang, N.Y. Huang, J.Z. Wang, et al., The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk. ) Pat, and its effects on gastric cancer cells, Fitoterapia. 89 (2013) 210-217. https://doi:10.1016/j.fitote.2013.05.021.
DOI Google Scholar
[3]

N.W. He, L.M. Tian, X.C. Zhai, et al., Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk. ) Pat, Int. J. Biol. Macromol. 115 (2018) 114-123. https://doi:10.1016/j.ijbiomac.2018.04.049.
DOI Google Scholar
[4]

P. Pronko, L. Bardina, V. Satanovskaya, et al., Effect of chronic alcohol consumption on the ethanol- and acetaldehyde-metabolizing systems in the rat gastrointestinal tract, Alcohol Alcohol. 37 (2002) 229-235. https://doi:10.1093/alcalc/37.3.229.
DOI Google Scholar
[5]

A. Stornetta, V. Guidolin, S. Balbo, Alcohol-derived acetaldehyde exposure in the oral cavity, Cancers (Basel). 10 (2018) 20-47. https://doi:10.3390/cancers10010020.
DOI Google Scholar
[6]

K.T. Suk, M.Y. Kim, S.K. Baik, Alcoholic liver disease: treatment, World J. Gastroenterol. 20 (2014) 12934-12944.
DOI Google Scholar
[7]

X. Liu, R.L. Hou, J.J. Yan, et al., Purification and characterization of Inonotus hispidus exopolysaccharide and its protective effect on acute alcoholic liver injury in mice, Int. J. Biol. Macromol. 129 (2019) 41-49. https://doi:10.1016/j.ijbiomac.2019.02.011.
DOI Google Scholar
[8]

R.L. Hou, X. Liu, J.J. Yan, et al., Characterization of natural melanin from Auricularia auricula and its hepatoprotective effect on acute alcohol liver injury in mice, Food Funct. 10 (2019) 1017-1027. https://doi:10.1039/C8FO01624K.
DOI Google Scholar
[9]

Y.M. Jin, X.M. Tao, Y.N. Shi, et al., Salvianolic acid B exerts a protective effect in acute liver injury by regulating the Nrf2/HO-1 signaling pathway, Can. J. Physiol. Pharmacol. 98 (2020) 162-168. https://doi:10.1139/cjpp-2019-0349.
DOI Google Scholar
[10]

L.Y. Ye, S.R. Liu, F. Xie, et al., Enhanced production of polysaccharides and triterpenoids in Ganoderma lucidum fruit bodies on induction with signal transduction during the fruiting stage, Plos One 13 (2018) e0196287. https://doi:10.1371/journal.pone.0196287.
DOI Google Scholar
[11]

X.Y. Ren, L. He, J.W. Cheng, et al., Optimization of the solid-state fermentation and properties of apolysaccharide from Paecilomyces cicadae (Miquel) samon and its antioxidant activities in vitro, Plos One. 9 (2014) e87578. https://doi:10.1371/journal.pone.0087578.
DOI Google Scholar
[12]

Y.L. Feng, W.Q. Li, X.Q. Wu, et al., Statistical optimization of media for mycelial growth and exo-polysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies, Biochem. Eng. J. 49 (2010) 104-112. https://doi:10.1016/j.bej.2009.12.002.
DOI Google Scholar
[13]

B.Z. Chen, B.R. Ke, L.Y. Ye, et al., Isolation and varietal characterization of Ganoderma resinaceum from areas of Ganoderma lucidum production in China, Sci. Hortic. 224 (2017) 109-114. https://doi:10.1016/j.scienta.2017.06.002.
DOI Google Scholar
[14]

M. Dubois, K.A. Gilles, J.K. Hamilton, et al., Colorimetric method for determination of sugars and related substances, Anal. Chem. 28 (1956) 350-356. https://doi:10.1021/ac60111a017.
DOI Google Scholar
[15]

N. Blumenkrantz, G. Asboe-Hansen, New method for quantitative determination of uronic acids, Anal. Biochem. 54 (1973) 484-489. https://doi:10.1016/0003-2697(73)90377-1.
DOI Google Scholar
[16]

M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254. https://doi:10.1016/0003-2697(76)90527-3.
DOI Google Scholar
[17]

H.N. Sun, T.H. Mu, X.L. Liu, et al., Purple sweet potato (Ipomoea batatas L. ) anthocyanins: preventive effect on acute and subacute alcoholic liver damage and dealcoholic effect, J. Agric. Food Chem. 62 (2014) 2364-2373. https://doi:10.1021/jf405032f.
DOI Google Scholar
[18]

Y.H. Xie, L.X. Wang, H. Sun, et al., Immunomodulatory, antioxidant and intestinal morphology-regulating activities of alfalfa polysaccharides in mice, Int. J. Biol. Macromol. 133 (2019) 1107-1114. https://doi:10.1016/j.ijbiomac.2019.04.144.
DOI Google Scholar
[19]

Z. Yun, H.X. Qu, H. Wang, et al., Comparative transcriptome and metabolome provides new insights into the regulatory mechanisms of accelerated senescence in litchi fruit after cold storage, Sci. Rep. UK. 6 (2016) 19356-19356. https://doi:10.1038/srep19356.
DOI Google Scholar
[20]

M. Pertea, D. Kim, G.M. Pertea, et al., Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown, Nat. Protoc. 11 (2016) 1650-1667. https://doi:10.1038/nprot.2016.095.
DOI Google Scholar
[21]

R.J. Hao, X.D. Du, C.Y. Yang, et al., Integrated application of transcriptomics and metabolomics provides insights into unsynchronized growth in pearl oyster Pinctada fucata martensii, Sci. Total Environ. 666 (2019) 46-56. https://doi:10.1016/j.scitotenv.2019.02.221.
DOI Google Scholar
[22]

H.H. Guo, H.X. Guo, L. Zhang, et al., Metabolome and transcriptome association analysis reveals dynamic regulation of purine metabolism and flavonoid synthesis in transdifferentiation during somatic embryogenesis in cotton, Int. J. Mol. Sci. 20 (2019) 2070-2093. https://doi:10.3390/ijms20092070.
DOI Google Scholar
[23]

J. Chong, O. Soufan, C. Li, et al., MetaboAnalyst 4.0: towards more transparent and integrative metabolomics analysis, Nucleic Acids Res. W1 (2018) W486-W494. https://doi:10.1093/nar/gky310.
DOI Google Scholar
[24]

Q.G. Meng, Y.H. Li, T.C. Xiao, et al., Antioxidant and antibacterial activities of polysaccharides isolated and purified from Diaphragma juglandis fructus, Int. J. Biol. Macromol. 105 (2017) 431-437. https://doi:10.1016/j.ijbiomac.2017.07.062.
DOI Google Scholar
[25]

R. Kasimu, C.L. Chen, X.Y. Xie, et al., Water-soluble polysaccharide from Erythronium sibiricum bulb: structural characterisation and immunomodulating activity, Int. J. Biol. Macromol. 105 (2017) 452-462. https://doi:10.1016/j.ijbiomac.2017.07.060.
DOI Google Scholar
[26]

W. Zheng, T. Zhao, W.W. Feng, et al., Purification, characterization and immunomodulating activity of a polysaccharide from flowers of Abelmoschus esculentus, Carbohydr. Polym. 106 (2014) 335-342. https://doi:10.1016/j.carbpol.2014.02.079.
DOI Google Scholar
[27]

S. Zhang, B. He, J.B. Ge, et al., Extraction, chemical analysis of Angelica sinensis polysaccharides and antioxidant activity of the polysaccharides in ischemia-reperfusion rats, Int. J. Biol. Macromol. 47 (2010) 546-550. https://doi:10.1016/j.ijbiomac.2010.07.012.
DOI Google Scholar
[28]

T. Zhao, G.H. Mao, W.W. Feng, et al., Isolation, characterization and antioxidant activity of polysaccharide from Schisandra sphenanthera, Carbohydr. Polym. 105 (2014) 26-33. https://doi:10.1016/j.carbpol.2014.01.059.
DOI Google Scholar
[29]

J. Chena, H.N. Suna, A.D. Suna, et al., Studies of the protective effect and antioxidant mechanism of blueberry anthocyanins in a CC14-induced liver injury model in mice, Food Agr. Immunol. 23 (2012) 352-362. https://doi:10.1080/09540105.2011.634378.
DOI Google Scholar
[30]

J.K. Marx, Oxygen free radicals linked to many diseases, Science 235 (1987) 529-531. https://doi:10.1126/science.3810154.
DOI Google Scholar
[31]

J.I. Beier, G.E. Arteel, C.J. McClain, Advances in alcoholic liver disease, Curr. Gastroenterol. Rep. 13 (2011) 56-64. https://doi:10.1007/s11894-010-0157-5.
DOI Google Scholar
[32]

J.L. Xiang, W.X. Zhu, Z.X. Li, et al., Effect of juice and fermented vinegar from Hovenia dulcis peduncles on chronically alcohol-induced liver damage in mice, Food Funct. 3 (2012) 628-634. https://doi:10.1039/C2FO10266H.
DOI Google Scholar
[33]

Q. Dong, F. Chu, C. Wu, et al., Scutellaria baicalensis Georgi extract protects against alcohol induced acute liver injury in mice and affects the mechanism of ER stress, Mol. Med. Rep. 13 (2016) 3052-3062. https://doi:10.3892/mmr.2016.4941.
DOI Google Scholar
[34]

C.L. Zhang, T. Zeng, X.L. Zhao, et al., Protective effects of garlic oil on hepatocarcinoma induced by N-nitrosodiethylamine in rats, Int. J. Bio. Sci. 8 (2012) 363-374. https://doi:10.7150/ijbs.3796.
DOI Google Scholar
[35]

K. Pradeep, C.V. Mohan, K. Gobianand, et al., Effect of Cassia fistula Linn. leaf extract on diethylnitrosamine induced hepatic injury in rats, Chem. Biol. Interact. 167 (2007) 12-18. https://doi:10.1016/j.cbi.2006.12.011.
DOI Google Scholar
[36]

J.K. Reddy, M.S. Rao, Lipid metabolism and liver inflammation. Ⅱ. Fatty liver disease and fatty acid oxidation, Am. J. Physiol. -Gastr. L. 290 (2006) G852-G858. https://doi:10.1152/ajpgi.00521.2005.
DOI Google Scholar
[37]

J. Sun, D.Y. Shi, S. Li, et al., Chemical constituents of the red alga Laurencia tristicha, J. Asian Nat. Prod. Res. 9 (2007) 725-734. https://doi:10.1080/10286020601103338.
DOI Google Scholar
[38]

N. Ge, H. Liang, Y.Y. Zhao, et al., Aplysin protects against alcohol-induced liver injury via alleviating oxidative damage and modulating endogenous apoptosis-related genes expression in rats, J. Food Sci. 83 (2018) 2612-2621. https://doi:10.1111/1750-3841.14320.
DOI Google Scholar
[39]

J. Yi, W. Xia, J.P. Wu, et al., Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice, J. Vet. Sci. 15 (2014) 141-148. https://doi:10.4142/jvs.2014.15.1.141.
DOI Google Scholar
[40]

X.Y. Zhao, L.Q. Wang, H. Zhang, et al., Protective effect of artemisinin on chronic alcohol induced-liver damage in mice, Environ. Toxicol. Phar. 52 (2017) 221-226. https://doi:10.1016/j.etap.2017.04.008.
DOI Google Scholar
[41]

S.H. Zeisel, K.A. Da Costa, P.D. Franklin, et al., Choline, an essential nutrient for humans, FASEB J. 5 (1991) 2093-2098.
DOI Google Scholar
[42]

R.J.Wurtman, M.J.Hirsch, J.H.Growdon, Lecithin consumption raises serum free choline levels, Lancet 2 (1977) 68-69.https://doi:10.1016/S0140-6736(77)90067-8.
DOI Google Scholar
[43]

D.J.Tuma, A.J.Barak, D.F.Schafer, et al., Possible interrelationship of ethanol metabolism and choline oxidation in the liver, Biochem.Cell Biol.51(1973) 117-120.https://doi:10.1139/o73-016.
DOI Google Scholar
[44]

R.Roberti, L.Binaglla, G.Michal, et al., The effect of acute ethanol ingestion on in vitro metabolism of choline and ethanolamine derivatives in rat liver, Biochem.Pharmacol.23 (1974) 3289-3298.https://doi:10.1016/0006-2952(74)90652-2.
DOI Google Scholar
[45]

S.F.Li, H.X.Liu, Y.B.Jin, et al., Metabolomics study of alcohol-induced liver injury and hepatocellular carcinoma xenografts in mice, J.Chromatogr.B.879 (2011) 2369-2375.https://doi:10.1016/j.jchromb.2011.06.018.
DOI Google Scholar
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 18 August 2020
Revised: 20 October 2020
Accepted: 25 October 2020
Published: 04 June 2021
Issue date: July 2021

Copyright

© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

This work was supported by the National Key Research and Development Program of China (grant number 2019YFC1710501), the construction of a modern agricultural industrial technology system in Fujian province and an expert workstation of the modern edible mushroom industrial technology systeminvolving Pleurotus pulmonarius and Ganoderma lucidum (project number Minnong General [2019] no. 144).

Rights and permissions

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Return