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Research Article | Open Access

The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics

Jinbin Hao1, Liyun Ye1, Guoliang Meng, Yanjiao Song, Junsheng Fu, Xiaoping Wu( )
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, China

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd

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Abstract

Trametes lactinea mycelia polysaccharides (TLMPS) have a wide range of bioactivities. The potential mechanisms of action of TLMPS against acute alcohol-induced liver injury in vivo were investigated by analyzing the physical and chemical properties of TLMPS and its protective effects on a mouse model of alcoholic liver injury. TLMPS protected the liver against alcohol-induced injury, as evidenced by the reduced alcohol-induced elevation of the liver index, serum biochemical indices, and maintenance of hepatic morphology. Potential mechanisms were analyzed using transcriptome and metabolome analyses. The transcriptome data revealed the involvement of many differentially expressed genes in chemical carcinogenesis, drug metabolism, and metabolism of xenobiotics by cytochrome P450. The metabolome analysis revealed that TLMPS significantly regulated specific metabolites in the liver, including organic acids, lipids, nucleosides, and organic oxygen compounds. KEGG enrichment analysis revealed the significant involvement of different metabolites in choline metabolism, ATP-binding cassette transporters, and glycerophospholipid metabolism. Assessment of the changes in gene expression and metabolites revealed the significantly different expression of several genes encoding key enzymes and metabolites in choline metabolism pathway. The collective findings confirmed that choline metabolism plays an important role in the protective effects of TLMPS against acute alcoholic liver injury.

Keywords

Trametes lactineaPolysaccharideHepatoprotectiveTranscriptomeMetabolome

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Food Science and Human Wellness
Volume 10 Issue 4,
July 2021
Pages 480-489
DOI: 10.1016/j.fshw.2021.04.010
Cite this article:
Hao J, Ye L, Meng G, et al. The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics. Food Science and Human Wellness, 2021, 10(4): 480-489. https://doi.org/10.1016/j.fshw.2021.04.010

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Received: 18 August 2020
Revised: 20 October 2020
Accepted: 25 October 2020
Published: 04 June 2021
© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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