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Food Science and Human Wellness 2021, 10(4): 452-459 https://doi.org/10.1016/j.fshw.2021.04.007
Research Article | Open Access | Issue | Published: 04 June 2021

Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress

Show Author's Information M. Subbulakshmia,  Sugapriya Dhanasekaranb( ),  S. Abiramic,  M. Kannand,  R. Palaniappane,  Divya Venugopalb
Department of Microbiology, K. R. College of Arts and Science, Kovilpatti, Tamil Nadu, India
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Wadi Al Dawaser, Saudi Arabia
Department of Microbiology, Kamaraj College, Thoothukudi, Tamil Nadu, India
Research Department of Zoology, V.H.N. Senthikumara Nadar College (Autonomus) Virudhunagar, Tamil Nadu, India
Research Department of Computer Science, V.H.N. Senthikumara Nadar College (Autonomus) Virudhunagar, Tamil Nadu, India

Peer review under responsibility of KeAi Communications Co., Ltd

Keywords:
Antioxidant, Thymol, Anti-proliferation, Dietary mushrooms, HeLa cells, Novel Mushrooms
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Subbulakshmi M, Dhanasekaran S, Abirami S, et al. Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress. Food Science and Human Wellness, 2021, 10(4): 452-459. https://doi.org/10.1016/j.fshw.2021.04.007
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Abstract Full text About this article

Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects. This study identified a unique isoform of 18S rRNA gene (864bp) from a novel wild mushroom (SMK-1) (GenBank accession number: SUB3267363). Thin layer chromatographic (TLC) profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and flavonoid fractions with retention factor (Rf) values of 0.955 and 0.927 respectively. The GC/MS chromatograms of the SMK-1 methanolic extract identified the main bioactive compound was phenol, 5-methyl-2-(1-methylethyl) (74.00%) (thymol). The radical scavenging activity for the flavonoid fraction was greater than the phenolic fractions (Rf–phenolics fractions > Rf–flavonoid fractions) with the antioxidant activity more than that of standard ascorbic acid. Also, the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5μg/mL to 80μg/mL in a dose-dependent manner. This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefits. The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug.


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About this article

Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress

Show Author's information M. Subbulakshmia, Sugapriya Dhanasekaranb( ), S. Abiramic, M. Kannand, R. Palaniappane, Divya Venugopalb
Department of Microbiology, K. R. College of Arts and Science, Kovilpatti, Tamil Nadu, India
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Wadi Al Dawaser, Saudi Arabia
Department of Microbiology, Kamaraj College, Thoothukudi, Tamil Nadu, India
Research Department of Zoology, V.H.N. Senthikumara Nadar College (Autonomus) Virudhunagar, Tamil Nadu, India
Research Department of Computer Science, V.H.N. Senthikumara Nadar College (Autonomus) Virudhunagar, Tamil Nadu, India

Peer review under responsibility of KeAi Communications Co., Ltd

Abstract

Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects. This study identified a unique isoform of 18S rRNA gene (864bp) from a novel wild mushroom (SMK-1) (GenBank accession number: SUB3267363). Thin layer chromatographic (TLC) profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and flavonoid fractions with retention factor (Rf) values of 0.955 and 0.927 respectively. The GC/MS chromatograms of the SMK-1 methanolic extract identified the main bioactive compound was phenol, 5-methyl-2-(1-methylethyl) (74.00%) (thymol). The radical scavenging activity for the flavonoid fraction was greater than the phenolic fractions (Rf–phenolics fractions > Rf–flavonoid fractions) with the antioxidant activity more than that of standard ascorbic acid. Also, the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5μg/mL to 80μg/mL in a dose-dependent manner. This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefits. The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug.

Keywords: Antioxidant, Thymol, Anti-proliferation, Dietary mushrooms, HeLa cells, Novel Mushrooms

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Received: 05 September 2020
Revised: 15 November 2020
Accepted: 01 December 2020
Published: 04 June 2021
Issue date: July 2021

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© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgment

The author is very thankful to the Deanship of Scientific Research, Prince Sattam bin Abdulaziz University for supporting this work. The author expresses hearty thanks to Our Team for their timely assistant and personally grateful to Mr. Muthamil Selvan for his understanding, full-hearted support and encouragement. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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