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Food Science and Human Wellness 2021, 10(1): 6-12 https://doi.org/10.1016/j.fshw.2020.08.007
Research Article | Open Access | Issue | Published: 25 September 2022

Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis

Show Author's Information Guliang Yanga,  Jianfeng Zhana( ),  Yiwen Yanga,  Li Yuana,  Peilei Wanga,  Chi-Tang Hob,  Shiming Lia,b( )
Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, China
Department of Food Science, Rutgers University, New Brunswick, NJ, USA

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Oxidative stress, Hepatic fibrosis, Oxyresveratrol, TGF-β/Smad/ERK signaling pathway
Cite this article:
Yang G, Zhan J, Yang Y, et al. Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis. Food Science and Human Wellness, 2021, 10(1): 6-12. https://doi.org/10.1016/j.fshw.2020.08.007
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Abstract Full text About this article

Oxyresveratrol (ORes, trans-2,4,3′,5′-tetrahydroxy stilbene) naturally exists in mulberry, grapes, peanuts and other plants. It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol (Res). Hence, ORes has stronger antioxidant activity than resveratrol. In present study, we employed a rat hepatic fibrosis model induced by carbon tetrachloride (CCl4) and administrated ORes via gavage feeding to study the protective effects and potential mechanisms of ORes against hepatic fibrosis. We demonstrated that rat liver oxidative damage induced by CCl4 was significantly alleviated after ORes feeding. Furthermore, the mRNA transcription levels of α-smooth muscle actinn (α-SMA), desmin, and two MMPs (MMP2 and MMP9) were reduced and the expression levels of transforming growth factor β1 (TGF-β1), p- small mother against decapen-taplegic protein (Smad)1/2 and p-extracellular signal-regulated kinases (ERK)1/2 in the liver tissue down-regulated dramatically. In a parallel study with Res, ORes showed more efficacious protective effect than Res against rat liver fibrosis, which is attributed to extended conjugation system due to the extra hydroxyl group at 2-position on ORes making it more electron-rich and susceptible to oxidation than Res. Therefore, dietary consumption of mulberry and other fruits containing ORes may be beneficial in the prevention of liver fibrosis.


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About this article

Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis

Show Author's information Guliang Yanga, Jianfeng Zhana( ), Yiwen Yanga, Li Yuana, Peilei Wanga, Chi-Tang Hob, Shiming Lia,b( )
Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, China
Department of Food Science, Rutgers University, New Brunswick, NJ, USA

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Oxyresveratrol (ORes, trans-2,4,3′,5′-tetrahydroxy stilbene) naturally exists in mulberry, grapes, peanuts and other plants. It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol (Res). Hence, ORes has stronger antioxidant activity than resveratrol. In present study, we employed a rat hepatic fibrosis model induced by carbon tetrachloride (CCl4) and administrated ORes via gavage feeding to study the protective effects and potential mechanisms of ORes against hepatic fibrosis. We demonstrated that rat liver oxidative damage induced by CCl4 was significantly alleviated after ORes feeding. Furthermore, the mRNA transcription levels of α-smooth muscle actinn (α-SMA), desmin, and two MMPs (MMP2 and MMP9) were reduced and the expression levels of transforming growth factor β1 (TGF-β1), p- small mother against decapen-taplegic protein (Smad)1/2 and p-extracellular signal-regulated kinases (ERK)1/2 in the liver tissue down-regulated dramatically. In a parallel study with Res, ORes showed more efficacious protective effect than Res against rat liver fibrosis, which is attributed to extended conjugation system due to the extra hydroxyl group at 2-position on ORes making it more electron-rich and susceptible to oxidation than Res. Therefore, dietary consumption of mulberry and other fruits containing ORes may be beneficial in the prevention of liver fibrosis.

Keywords: Oxidative stress, Hepatic fibrosis, Oxyresveratrol, TGF-β/Smad/ERK signaling pathway

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Publication history

Received: 27 June 2020
Revised: 16 August 2020
Accepted: 20 August 2020
Published: 25 September 2022
Issue date: January 2021

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© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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Acknowledgement

Grant from Hubei Province, China (GRANT number 2019ABA100)

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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