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Food Science and Human Wellness 2021, 10(1): 63-71 https://doi.org/10.1016/j.fshw.2020.05.003
Research Article | Open Access | Issue | Published: 06 July 2020

Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea

Show Author's Information Jingna Wua,b( ),  Xiaoting Chenc,  Kun Qiaoc,  Yongchang Suc,  Zhiyu Liuc( )
Xiamen Key Laboratory of Marine Medicinal Natural Products Resources, Xiamen Medical College, 361023, Xiamen, China
Fujian Universities and Colleges Engineering Research Center of Marine Biopharmaceutical Resources, Xiamen Medical College, 361023, Xiamen, China
Fisheries Research Institute of Fujian, 361013, Xiamen, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Polysaccharide, Bangia fuscopurpurea, Structure, ovarian cancer, Antitumor effect
Cite this article:
Wu J, Chen X, Qiao K, et al. Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea. Food Science and Human Wellness, 2021, 10(1): 63-71. https://doi.org/10.1016/j.fshw.2020.05.003
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Abstract Full text About this article

A water-soluble polysaccharide, designated BFP-3, was isolated from Bangia fuscopurpurea by hot water extraction, anion-exchange, and size-exclusion chromatography and tested to determine its antitumor activity. The structural characteristics of BFP-3 were investigated by chemical and spectroscopic methods, including partial acid hydrolysis, methylation analysis, one- and two-dimensional nuclear magnetic resonance, and gas chromatography-mass spectrometry. The results showed that BFP-3 was mainly comprised of rhamnose, arabinose, mannose, glucose, and galactose. Moreover, the weight-average molecular weight of BFP-3 was estimated to be approximately 333 kDa. The backbone of BFP-3 was primarily composed of repeating 5-α-l-Araf-1→(4-α-d-Glcp-1)4→4,6-β-d-Manp-1 units, and the side chains consisted of repeating β-d-Galp-1→(4-β-d-Galp-1)4→4,6-β- d-Galp-1→3,4-α-l-Rhap, β-l-Arap-1→(3-β-d-Galp-1)3, and β-l-Arap-1 units. Counting Kit-8 assays revealed that BFP-3 significantly inhibited the proliferation of A2780, COC1, SKOV3, HO-8910, and OVCAR3 ovarian cancer cells in vitro, indicating that BFP-3 could have potential applications in the treatment of ovarian cancer.


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Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea

Show Author's information Jingna Wua,b( ), Xiaoting Chenc, Kun Qiaoc, Yongchang Suc, Zhiyu Liuc( )
Xiamen Key Laboratory of Marine Medicinal Natural Products Resources, Xiamen Medical College, 361023, Xiamen, China
Fujian Universities and Colleges Engineering Research Center of Marine Biopharmaceutical Resources, Xiamen Medical College, 361023, Xiamen, China
Fisheries Research Institute of Fujian, 361013, Xiamen, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

A water-soluble polysaccharide, designated BFP-3, was isolated from Bangia fuscopurpurea by hot water extraction, anion-exchange, and size-exclusion chromatography and tested to determine its antitumor activity. The structural characteristics of BFP-3 were investigated by chemical and spectroscopic methods, including partial acid hydrolysis, methylation analysis, one- and two-dimensional nuclear magnetic resonance, and gas chromatography-mass spectrometry. The results showed that BFP-3 was mainly comprised of rhamnose, arabinose, mannose, glucose, and galactose. Moreover, the weight-average molecular weight of BFP-3 was estimated to be approximately 333 kDa. The backbone of BFP-3 was primarily composed of repeating 5-α-l-Araf-1→(4-α-d-Glcp-1)4→4,6-β-d-Manp-1 units, and the side chains consisted of repeating β-d-Galp-1→(4-β-d-Galp-1)4→4,6-β- d-Galp-1→3,4-α-l-Rhap, β-l-Arap-1→(3-β-d-Galp-1)3, and β-l-Arap-1 units. Counting Kit-8 assays revealed that BFP-3 significantly inhibited the proliferation of A2780, COC1, SKOV3, HO-8910, and OVCAR3 ovarian cancer cells in vitro, indicating that BFP-3 could have potential applications in the treatment of ovarian cancer.

Keywords: Polysaccharide, Bangia fuscopurpurea, Structure, ovarian cancer, Antitumor effect

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Publication history

Received: 02 February 2020
Revised: 29 April 2020
Accepted: 04 May 2020
Published: 06 July 2020
Issue date: January 2021

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© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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Acknowledgements

This work was funded by the Science and Technology Project of Xiamen Medical College (K2016-36).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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