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Rubus fruits are rich in nutrition with a wide range of phytochemical and consumed by humans, but many of its wild varieties are not commercially useful. Phytochemical exploration of these wild fruits could improve the commercial usage of these Rubus species.This study focuses to analyze the phytochemical, antioxidant and cytotoxicity of three wild Rubus ellipticus, R. niveus and R. fairholmianus fruits comparatively, which has not revealed. The in vitro antioxidant studies indicated the ethyl acetate and methanol extracts of studied Rubus species competent in scavenging different stable and generated radical. The correlation and regression analysis also statistically supported the contents in the extracts could act against various radicals. Correlation between phytochemical constituent and radical scavenging activity found to be highly positive. The results acquired from this investigation revealed that they are equally competent and could be recommended as dietary supplements or as nutraceuticals.


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Phenolics, tannins, flavonoids and anthocyanins contents influenced antioxidant and anticancer activities of Rubus fruits from Western Ghats, India

Show Author's information Kasipandi Muniyandia,Elizabeth Georgea,bSaikumar SathyanarayananaBlassan P. GeorgecHeidi AbrahamsecSuman ThamburajaParimelazhagan Thangaraja( )
Bioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu 641046, India
Department of Botany, Mar Thoma College, Chungathara, Kerala 679334, India
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Rubus fruits are rich in nutrition with a wide range of phytochemical and consumed by humans, but many of its wild varieties are not commercially useful. Phytochemical exploration of these wild fruits could improve the commercial usage of these Rubus species.This study focuses to analyze the phytochemical, antioxidant and cytotoxicity of three wild Rubus ellipticus, R. niveus and R. fairholmianus fruits comparatively, which has not revealed. The in vitro antioxidant studies indicated the ethyl acetate and methanol extracts of studied Rubus species competent in scavenging different stable and generated radical. The correlation and regression analysis also statistically supported the contents in the extracts could act against various radicals. Correlation between phytochemical constituent and radical scavenging activity found to be highly positive. The results acquired from this investigation revealed that they are equally competent and could be recommended as dietary supplements or as nutraceuticals.

Keywords: Antioxidant, Flavonoids, Anticancer, Phenolics, Nutraceuticals

References(30)

[1]

S. Saklani, S. Chandra, A.P. Mishra, Evaluation of nutritional profile, medicinal value and quantitative estimation in different parts of Pyrus pashia, Ficus palmata and Pyracantha crenulata, Asian J. Chem. 2 (3) (2011) 350–354.

[2]

L.B. Carvalho-Silva, M.V. Oliveira, V.S. Gontijo, et al., Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis, Food Res. Int. 48 (2012) 180–186.

[3]
R.L. Prior, Absorption and metabolism of anthocyanins: potential health effects, in: M. Meskin, W.R. Bidlack, A.J. Davies, D.S. Lewis, R.K. Randolph (Eds. ), Phytochemicals: Mechanism of Action, CRC Press, Inc., Boca Raton, USA, 2004, pp. 19–20.
DOI
[4]

J.M. Sasikumar, V. Maheshu, A.G. Smilin, et al., Antioxidant and antihaemolytic activities of common Nilgiri barberry (Berberis tinctoria Lesch. ) from South India, Int. Food Res. J. 19 (2012) 1601–1607.

[5]

B.P. George, T. Parimelazhagan, R. Chandran, Anti-inflammatory and wound healing properties of Rubus fairholmianus Gard. Root-An in vivo study, Ind. Crops Prod. 54 (2014) 216–225.

[6]

B.P. George, T. Parimelazhagan, Y.T. Kumar, et al., Antitumor and wound healing properties of Rubus ellipticus smith, J. Acupunct. Meridian Stud. 8 (3) (2015) 134–141.

[7]

B.P. George, T. Parimelazhagan, S. Saravanan, et al., Anti-inflammatory, analgesic and antipyretic properties of Rubus niveus Thunb. root acetone extract, Pharmacologia 4 (2013) 228–235.

[8]

S.K. Uniyal, K.N. Singh, P. Jamwal, et al., Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya, J. Ethnobiol. Ethnomed. 2 (1) (2006) 14.

[9]
H.P.S. Makkar, Quantification of Tannins in Tree and Shrub Foliage: A Laboratory Mannual, Dondrecht. Kluwer Academic Publishers, The Netherlands, 2003.
DOI
[10]

J. Zhishen, T. Mengecheng, W. Jianming, The determination of flavonoid contents on mulberry and their scavenging effects on superoxide radical, Food Chem. 64 (1999) 555–559.

[11]

B. Du, B.J. He, P.B. Shi, et al., Phenolic content and antioxidant activity of wine grapes and table grapes, J. Med. Plants Res. 6 (2012) 3381–3387.

[12]

M.S. Blois, Antioxidants determination by the use of a stable free radical, Nature 4617 (1958) 1199–1200.

[13]

R. Re, N. Pellegrini, A. Proteggente, et al., Antioxidant activity applying an improved ABTS radical cation decolourization assay, Free Radic. Biol. Med. 26 (1999) 1231–1237.

[14]

R. Pulido, L. Bravo, F. Sauro-Calixto, Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing and antioxidant power assay, J. Agric. Food Chem. 48 (2000) 3396–3402.

[15]

P. Prieto, M. Pineda, M. Aguilar, Spectophotometric quantitative of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E, Anal. Biochem. 269 (1999) 337–341.

[16]

M. Kasipandi, E. George, M. Vekataramana, et al., Antioxidant and anticancer activities of Plectranthus stocksii Hook. f. leaf and stem extracts, Agric. Nat. Resour. 51 (2) (2017) 63–73.

[17]

A. Hubaux, G. Vos, Decision and detection limits for calibration curves, Anal. Chem. 42 (8) (1970) 849–855.

[18]

E. George, M. Kasipandi, M. Vekataramana, et al., In vitro anti-oxidant and cytotoxic analysis of Pogostemon mollis Benth, Bangladesh J. Pharmacol. 11 (1) (2016) 148–158.

[19]

J. Dai, R.J. Mumper, Plant Phenolics: extraction, analysis and their antioxidant and anticancer properties, Molecules 15 (2010) 7313–7352.

[20]

K.T. Chung, T.Y. Wong, C.I. Wei, et al., Tannins and human health: a review, Crit. Rev. Food Sci. Nutr. 38 (1998) 421–464.

[21]

, C. Kandaswami, Effects of flavonoids on immune and inflammatory cell functions, Biochem. Pharmacol. 43 (1992) 1167–1179.

[22]

A.E. Hagerman, K.M. Reidl, G.A. Jones, et al., High molecular weight polyphenolics (tannins) as biological antioxidants, J. Agric. Food Chem. 46 (1998) 1887–1892.

[23]

M.M.B. Almeida, P.H.M. de Sousa, A.M.C. Arriaga, et al., Bioactive compounds and antioxidant activity of fresh exotic fruits from Northeastern Brazil, Food Res. Int. 44 (2011) 2155–2159.

[24]

F.G.K. Vieira, P.F. Di Pietro, E.L. da Silva, et al., Improvement of serum antioxidant status in humans after the acute intake of apple juices, Nutr. Res. 32 (2012) 229–232.

[25]

A. Mansouri, G. Embarek, E. Kokkalou, et al., Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera), Food Chem. 89 (2005) 411–420.

[26]

C.S. Bowen-Forbes, Y. Zhang, M.G. Nair, Anthocyanin content, antioxidant, anti-inflammatory and anticancer properties of blackberry and raspberry fruits, J. Food Compos. Anal. 23 (6) (2010) 554–560.

[27]

R. Byamukama, B.T. Kiremire, Ø. M. Andersen, et al., Anthocyanins from fruits of Rubus pinnatus and Rubus rigidus, J. Food Compos. Anal. 18 (6) (2005) 599–605.

[28]

R.A. Moyer, K.E. Hummer, C.E. Finn, et al., Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, Rubus, and Ribes, J. Agric. Food Chem. 50 (3) (2002) 519–525.

[29]

B.P. George, T. Parimelazhagan, R. Chandran, Evaluation of total phenolic content, antioxidant and analgesic potential of Rubus fairholmianus gard, Int. J. Pharm. Pharm. Sci. 5 (3) (2013) 484–488.

[30]

R. Zhong, Q. Guo, G. Zhou, et al., Three new labdane-type diterpene glycosides from fruits of Rubus chingii and their cytotoxic activities against five humor cell lines, Fitoterapia 102 (2015) 23–26.

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Publication history

Received: 11 September 2018
Revised: 04 March 2019
Accepted: 06 March 2019
Published: 25 March 2019
Issue date: March 2019

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© 2019 “Society information”.

Acknowledgements

Acknowledgement

All the authors acknowledge the University Grants Commission (UGC)–Special Assistance Programme [Ref No: F.5-16/2016/DRS-1(SAP-II)] in Department of Botany, Bharathiar University, Coimbatore, India for granting the instrument facilities during the research work. The first author gratefully acknowledges to University Grants Commission, India for the Rajiv Gandhi National Fellowship (Ref No. F1-17.1/2017-18/RGNF-2017-18-SC-TAM-37934).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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