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Food Science and Human Wellness 2018, 7(4): 273-281 https://doi.org/10.1016/j.fshw.2018.10.004
Research Article | Open Access | Issue | Published: 03 November 2018

Anti-inflammatory activity of Alpinia officinarum hance on rat colon inflammation and tissue damage in DSS induced acute and chronic colitis models

Show Author's Information Vijayabharathi Rajendiran,  Vidhya Natarajan,  Sivasithamparam Niranjali Devaraj( )
Department of Biochemistry, University of Madras, Maraimalai Campus (Guindy), Chennai, 600025, India

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:
Inflammation, Antioxidants, Alpinia officinarum, Dextran sulfate sodium (DSS), Inflammatory bowel diseases (IBD)
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Rajendiran V, Natarajan V, Devaraj SN. Anti-inflammatory activity of Alpinia officinarum hance on rat colon inflammation and tissue damage in DSS induced acute and chronic colitis models. Food Science and Human Wellness, 2018, 7(4): 273-281. https://doi.org/10.1016/j.fshw.2018.10.004
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Abstract Full text About this article

The purpose of this study was to investigate the possible prophylactic effects of Alpinia officinarum hance on experimentally induced acute and chronic colitis models, in-vivo and in-vitro. Acute and chronic colitis were induced in Male Wistar rats by administration of Dextran Sulfate Sodium (DSS) in drinking water. DSS induction exhibited colon shrinkage, increased the Disease Activity Index (DAI) score, increased the levels of inflammatory markers and caused severe anemia. DSS induced animals, co-treated with the hexane extract of Alpinia officinarum (HEAO) (200 mg/kg body wt), effectively suppressed colonic injury that was evidenced by the reduced DAI score, colon weight/length ratio, histological damage, proinflammatory markers and MPO activity. Further, it restored the colonic antioxidants near to normal levels by regulating the oxidative stress via attenuation of lipid peroxidation. Our results revealed that the degree of colitis caused by the administration of DSS was significantly attenuated by HEAO. In addition, the in-vitro study showed that HEAO treatment inhibited the proliferation of HT-29 cells and down regulated the mRNA expression of NF-κB and COX-2. Taken together, these results suggest that HEAO is a promising anti-oxidant and anti-inflammatory agent that support its possible therapeutic role in the treatment of colitis.


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About this article

Anti-inflammatory activity of Alpinia officinarum hance on rat colon inflammation and tissue damage in DSS induced acute and chronic colitis models

Show Author's information Vijayabharathi Rajendiran, Vidhya Natarajan, Sivasithamparam Niranjali Devaraj( )
Department of Biochemistry, University of Madras, Maraimalai Campus (Guindy), Chennai, 600025, India

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

The purpose of this study was to investigate the possible prophylactic effects of Alpinia officinarum hance on experimentally induced acute and chronic colitis models, in-vivo and in-vitro. Acute and chronic colitis were induced in Male Wistar rats by administration of Dextran Sulfate Sodium (DSS) in drinking water. DSS induction exhibited colon shrinkage, increased the Disease Activity Index (DAI) score, increased the levels of inflammatory markers and caused severe anemia. DSS induced animals, co-treated with the hexane extract of Alpinia officinarum (HEAO) (200 mg/kg body wt), effectively suppressed colonic injury that was evidenced by the reduced DAI score, colon weight/length ratio, histological damage, proinflammatory markers and MPO activity. Further, it restored the colonic antioxidants near to normal levels by regulating the oxidative stress via attenuation of lipid peroxidation. Our results revealed that the degree of colitis caused by the administration of DSS was significantly attenuated by HEAO. In addition, the in-vitro study showed that HEAO treatment inhibited the proliferation of HT-29 cells and down regulated the mRNA expression of NF-κB and COX-2. Taken together, these results suggest that HEAO is a promising anti-oxidant and anti-inflammatory agent that support its possible therapeutic role in the treatment of colitis.

Keywords: Inflammation, Antioxidants, Alpinia officinarum, Dextran sulfate sodium (DSS), Inflammatory bowel diseases (IBD)

References(42)

[1]

M.S. Geier, R.N. Butler, G.S. Howarth, Inflammatory bowel disease: current insights into pathogenesis and new therapeutic options; probiotics, prebiotics and synbiotics, Int. J. Food Microbiol. 115 (2007) 1–11.
DOI Google Scholar
[2]

D.K. Podolsky, Inflammatory bowel disease, N. Engl. J. Med. 347 (2002) 417–429.
DOI Google Scholar
[3]

Phil-Sun Oh and Kye-Taek Lim, Plant originated glycoprotein has anti-oxidative and anti-inflammatory eff; ;ects on dextran sulfate sodium-induced colitis in mouse, J. Biomed. Sci. 13 (549–560) (2006) 549.
DOI Google Scholar
[4]

I. Okayasu, S. Hatakeyama, M. Yamada, T. Ohkusa, Y. Inagaki, R. Nakaya, A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice, Gastroenterology 98 (3) (1990) 694–702.
DOI Google Scholar
[5]

L.G. Axelsson, E. Landstrom, T.J. Goldschmidt, A. Gronberg, A.C. Bylund-Fellenius, Dextran sulfate sodium (DSS) induced experimental colitis in immunodeficient mice: effects in CD4(+) -cell depleted, athymic and NK-cell depleted SCID mice, Inflamm. Res. 45 (1996) 181–191.
DOI Google Scholar
[6]

Tae Woon Kim, Jae Nam Seo, Young Ho Suh, Hyo Jin Park, Ju Hyun Kim, Ji Young Kim, Oh Kwon Ik, Involvement of lymphocytes in dextran sulfate sodium-induced experimental colitis, World J. Gastroenterol. 12 (2) (2006) 302–305.
DOI Google Scholar
[7]

Tzou-Chi Huang, Shinn-Shyong Tsai, Li-Fang Liu, Yu Lin Liu, Hung-Jen Liu, Kuo Pin Chuang, Effect of Arctium lappa L. in the dextran sulfate sodium colitis mouse model, World J. Gastroenterol. 16 (33) (2010) 4193–4199.
DOI Google Scholar
[8]

S.B. Hanauer, Inflammatory bowel disease, N. Engl. J. Med. 334 (1996) 841e8.
DOI Google Scholar
[9]

M. John, Pezzuto, Plant-derived anticancer agents, Biochem. Pharmacol. 53 (2) (1997) 121–133.
DOI Google Scholar
[10]

S. Ghosh, L. Rangan, Alpinia: the gold mine of future therapeutics, Biotechnology 3 (2013) 173.
DOI Google Scholar
[11]

Ning An, Hong-wu Zhang, Li-zhen Xu, Shi-lin Yang, Zhong-mei Zou, New diarylheptanoids from the rhizome of Alpinia officinarum Hance, Food Chem. 119 (2010) 513–517.
DOI Google Scholar
[12]

Griangsak Eumkeb, Santi Sakdarat, Supatcharee Siriwong, Reversing β-lactam antibiotic resistance of Staphylococcus aureus with galangin from Alpinia officinarum Hance and synergism with ceftazidime, Phytomedicine 18 (2010) 40–45.
DOI Google Scholar
[13]

Kacey Reid, Vincent Wright, Samson Omoregie, Anticancer Properties of Alpinia officinarum (Lesser Galangal) – a mini review, Int. J. Adv. Res. 4 (2016) 300–306.
DOI Google Scholar
[14]

Sabine Mercier, Denis Breuille, Laurent Mosoni, Christiane Obled, Philippe Patureau Mirand, Chronic inflammation alters protein metabolism in several organs of adult rats, Nutr. Metab. J. Nutr. 132 (2002) 1921–1928.
DOI Google Scholar
[15]

H.S. Cooper, S.N. Murthy, R.S. Shah, D.J. Sedergran, Clinicopathologic study of dextran sulfate sodium experimental murine colitis, Lab. Invest. 69 (1993) 238–249.
Google Scholar
[16]
B.A. Brown, Textbook of Hematology Principle Procedures, 2nd edition, Lea and Febiger, Philadelphia. USA, 1976, pp. 56–81.
[17]
S.G. Hewitt, Manual for veterinary investigation, hematology and laboratory techniques, in: Bulleting of Ministry of Agric. Fishery, Food and Hematology, 3rd edition, 1984, pp. 77–79.
[18]
O.W. Schalm, N.C. Jain, E.J. Caroll, Textbook of Veterinary Hematology, 3rd edition, 1975.
[19]
J. King, The hydrolases-acid and alkaline phosphatases in: Practical Clinical Enzymology, D Van Nostrand Co., London, 1965, 199e208.
[20]
J. King, Lactate dehydrogenase, in: Practical Clinical Enzymology, D. Van Nostrand Co., London, 1965, pp. 3e93.
[21]

S.B. Rosali, D. Rau, Serum gamma-glutamyl transpeptidase activity in alcoholism, Clin. Chim. Acta 39 (1972) 41–47.
DOI Google Scholar
[22]
H.U. Bergmeyer, E. Bernt, Aminotransferases and related enzymes, in: H.U. Bergmeyer (Ed. ), Methods of Enzymatic Analysis, 2nd ed., Academic Press, New York, 1974, pp. 735–763.
DOI
[23]

P.P. Bradley, D.A. Priebat, R.D. Christensen, G. Rothstein, Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker, J. Invest. Dermatol. 78 (1982) 206–209.
DOI Google Scholar
[24]

J. Sedlak, R.H. Lindsay, Estimation of total protein bound and nonprotein sulfhydryl groups in tissues with Ellman's reagent, Anal. Biochem. 25 (1968) 192–205.
DOI Google Scholar
[25]

M.S. Moron, J.W. Depierre, B. Mannervik, Levels of glutathione, glutathione reductase and glutathione- S-transferase activities in rat lung and liver, Chin. J. Biochem. Biophys. 582 (1979) 67–78.
DOI Google Scholar
[26]

H.P. Misra, I. Fridovich, The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase, J. Biol. Chem. 247 (1972) 3170–3175.
DOI Google Scholar
[27]

S. Takahara, H.B. Hamilton, J.V. Neel, T.Y. Kobara, Y. Ogura, E.T. Nishimura, Hypocatalasemia: a new genetic carrier state, J. Clin. Invest. 39 (1960) 610–619.
DOI Google Scholar
[28]

J. Rotruck, A.L. T Pope, H.E. Ganther, A.B. Swanson, D.G. Hafeman, W.G. Hoekstra, Selenium: biochemical role as a component of glutathione peroxidise, Science 179 (1973) 588–590.
DOI Google Scholar
[29]

W.H. Habig, M.J. Pabst, W.B. Jakoby, Glutathione S-transferases: the first enzymatic step in mercapturic acid formation, J. Biol. Chem. 249 (22) (1974) 7130–7139.
DOI Google Scholar
[30]

H. Ohkawa, N. Ohishi, K. Yagi, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem. 95 (1979) 351–358.
DOI Google Scholar
[31]

C.O. Beauchamp, I. Fridovich, Superoxide dismutase: improved assays and an assay applicable to acrylamide gels, Anal. Biochem. 44 (1) (1971) 276–287.
DOI Google Scholar
[32]

Y. Sun, Free radicals, antioxidant enzymes, and carcinogenesis, Free Rad. Biol. Med. 8 (1990) 583–599.
DOI Google Scholar
[33]

T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods 65 (1–2) (1983) 55–63.
DOI Google Scholar
[34]

S. Ashorn, T. Honkanen, K.L. Kolho, M. Ashorn, T. Valineva, B. Wei, J. Braun, I. Rantala, T. Luukkaala, S. Iltanen, Fecal calprotectin levels and serological responses to microbial antigens among children and adolescents with inflammatory bowel disease, Inflamm. Bowel Dis. 15 (2) (2009) 199–205.
DOI Google Scholar
[35]

V. Uko, S. Thangada, K. Radhakrishnan, Liver disorders in inflammatory bowel disease, Gastroenterol. Res. Pract. (2012) 642923.
DOI Google Scholar
[36]

S. Wirtz, M.F. Neurath, Mouse models of inflammatory bowel disease, Adv. Drug Delivery Rev. 59 (2007) 1073–1083.
DOI Google Scholar
[37]

L. Camacho-Barquero, I. Villegas, J.M. Sanchez-Calvo, E. Talero, S. Sanchez-Fidalgo, V. Motilva, C. Alarcon de la Lastra, Curcumin, a Curcuma longa constituent, acts on MAPK p38 pathway modulating COX-2 and iNOS expression in chronic experimental colitis, Int. Immunopharmacol. 7 (2007) 333–342.
DOI Google Scholar
[38]

E. Talero, S. Sanchez-Fidalgo, J. Ramon Calvo, V. Motilva, Galanin in the trinitrobenzene sulfonic acid rat model of experimental colitis, Int. Immunopharmacol. 1 (6) (2006) 1404–1412.
DOI Google Scholar
[39]

A.K. Pandurangan, Z. Saadatdoust, H. Hamzah, A. Ismail, Dietary cocoa protects against colitis associated cancer by activating the Nrf2/Keap1 pathway, BioFactors 41 (2015) 14.
DOI Google Scholar
[40]

A. DOdorico, S. Bortolan, R. Cardin, R. DInca, D. Martines, A. Ferronato, G.C. Sturniolo, Reduced plasma antioxidant concentrations and increased oxidative DNA damage in inflammatory bowel disease, Scand. J. Gastroenterol. 12 (2001) 1289–1294.
DOI Google Scholar
[41]

I. Masoodi, R. Kochhar, U. Dutta, C. Vaishnavi, K.K. Prasad, K. Vaiphei, S. Kaur, K. Singh, Fecal lactoferrin, myeloperoxidase and serum C-reactive are effective biomarkers in the assessment of disease activity and severity in patients with idiopathic ulcerative colitis, J. Gastroenterol. Hepatol. 24 (11) (2009) 1768–1774.
DOI Google Scholar
[42]

J.C. Hardwick, G.R. van den Brink, G.J. Offerhaus, S.J. van Deventer, M.P. Peppelenbosch, NF-κB, p38 MAPK and JNK are highly expressed and active in the stroma of human colonic adenomatous polyps, Oncogene 20 (2001) 819–827.
DOI Google Scholar
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Received: 09 July 2018
Revised: 28 October 2018
Accepted: 31 October 2018
Published: 03 November 2018
Issue date: December 2018

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