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Food Science and Human Wellness 2017, 6(2): 60-69 https://doi.org/10.1016/j.fshw.2017.02.001
Research Article | Open Access | Issue | Published: 24 February 2017

Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC)

Show Author's Information S. Jamunaa,  M.S. Sakeena Sadullaha,  R. Ashokkumara,  Shanmuganathan Gokulb,  Mozhi Senguttuvan Sivanb,  S. Niranjali Devaraja( )
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, India
Department of Biotechnology, Prist University, Puducherry, India

Peer review under responsibility of Beijing Academy of Food Sciences.

Keywords:
Antioxidants, Cymbopogon citratus, Cytoprotective, PBMC, Modified LDL
Cite this article:
Jamuna S, Sakeena Sadullah M, Ashokkumar R, et al. Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC). Food Science and Human Wellness, 2017, 6(2): 60-69. https://doi.org/10.1016/j.fshw.2017.02.001
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Abstract Full text About this article

Cymbopogon citratus (lemon grass) is commonly used in traditional folk medicine. The essential oil extracted from C. citratus has been reported as a potential anti-oxidant and anti-inflammatory agent. This study has been designed to explore the protective effect of C. citratus (lemon grass) against modified LDL (OxLDL and H2O2 LDL) induced cytotoxicity in Peripheral Blood Mononuclear Cells (PBMC). The essential oil extracted from C. citratus (EOC) was subjected to FT-IR spectroscopic analysis for the identification of functional groups. In vitro antioxidant assays were carried out to assess the electron donating capability of EOC as compared with a known standard L-ascorbic acid. The cytoprotective effects of EOC were determined in PBMC induced with modified LDL. Spectra obtained from FT-IR analysis showed the presence of functional groups in EOC such as H-bonded, O—H stretching, N—H stretching, aldehyde—C—H stretching, aldehyde/ketone—C═O stretching, —C═C-stretching, —CH3 bending, —C—H in plane bending. EOC has greater antioxidant property when compared with the standard L-ascorbic acid. EOC at all test concentrations demonstrated free radical scavenging activity and cytoprotective effect when challenged against modified LDL in PBMC. The above results show EOC as a promising antioxidant and cytoprotective agent.


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Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC)

Show Author's information S. Jamunaa, M.S. Sakeena Sadullaha, R. Ashokkumara, Shanmuganathan Gokulb, Mozhi Senguttuvan Sivanb, S. Niranjali Devaraja( )
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, India
Department of Biotechnology, Prist University, Puducherry, India

Peer review under responsibility of Beijing Academy of Food Sciences.

Abstract

Cymbopogon citratus (lemon grass) is commonly used in traditional folk medicine. The essential oil extracted from C. citratus has been reported as a potential anti-oxidant and anti-inflammatory agent. This study has been designed to explore the protective effect of C. citratus (lemon grass) against modified LDL (OxLDL and H2O2 LDL) induced cytotoxicity in Peripheral Blood Mononuclear Cells (PBMC). The essential oil extracted from C. citratus (EOC) was subjected to FT-IR spectroscopic analysis for the identification of functional groups. In vitro antioxidant assays were carried out to assess the electron donating capability of EOC as compared with a known standard L-ascorbic acid. The cytoprotective effects of EOC were determined in PBMC induced with modified LDL. Spectra obtained from FT-IR analysis showed the presence of functional groups in EOC such as H-bonded, O—H stretching, N—H stretching, aldehyde—C—H stretching, aldehyde/ketone—C═O stretching, —C═C-stretching, —CH3 bending, —C—H in plane bending. EOC has greater antioxidant property when compared with the standard L-ascorbic acid. EOC at all test concentrations demonstrated free radical scavenging activity and cytoprotective effect when challenged against modified LDL in PBMC. The above results show EOC as a promising antioxidant and cytoprotective agent.

Keywords: Antioxidants, Cymbopogon citratus, Cytoprotective, PBMC, Modified LDL

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Publication history
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Publication history

Received: 16 July 2016
Revised: 12 January 2017
Accepted: 06 February 2017
Published: 24 February 2017
Issue date: June 2017

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© 2017 Beijing Academy of Food Sciences.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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