Journal Home > Volume 5 , Issue 4

Xylanase is a hemicellulase that can hydrolyses the complex polysaccharides. Hemicelluloses are main components of cell walls of cereal grains. Moreover, hemicelluloses are considered as potential sources of mono- and oligosaccharides. In this study, influence of xylanase on the physicochemical properties and sensory qualities of the whole wheat bread during storage was investigated. Studies of whole wheat bread on microstructure, texture, thermotics, Scanning Electron Microscopic (SEM), X-Ray Diffraction (XRD) were conducted at ambient temperature of 25 and 4 °C respectively. During storage at different temperatures, bread containing xylanase exhibited less firmness but larger volume with whiter crumb color and longer shelf life as compared to control bread. Results of firmness, enthalpy, Fourier Transformation Infra Red (FTIR) and X-Ray Diffraction (XRD) studies suggested a lower staling rate of bread containing xylanase as compared to control one. Bread containing xylanase showed a smoother surface and more uniform pore size than the control. Significant differences in microstructure of control and bread containing xylanase were observed which might be attributed due to the change in water starch gluten interaction. These differences were also found to be interrelated to the textural properties of bread. Better sensory features were achieved in bread containing xylanase.


menu
Abstract
Full text
Outline
About this article

Thermo-mechanical and micro-structural properties of xylanase containing whole wheat bread

Show Author's information G. Ghoshala( )U.S. ShivhareaU.C. Banerjeeb
Dr. S.S. Bhatnagar University Institute of Chemical Engineering and Technology, Panjab University, Chandigarh 160014, India
Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research, SAS Nagar 160062, Punjab, India

Peer review under responsibility of Beijing Academy of Food Sciences.

Abstract

Xylanase is a hemicellulase that can hydrolyses the complex polysaccharides. Hemicelluloses are main components of cell walls of cereal grains. Moreover, hemicelluloses are considered as potential sources of mono- and oligosaccharides. In this study, influence of xylanase on the physicochemical properties and sensory qualities of the whole wheat bread during storage was investigated. Studies of whole wheat bread on microstructure, texture, thermotics, Scanning Electron Microscopic (SEM), X-Ray Diffraction (XRD) were conducted at ambient temperature of 25 and 4 °C respectively. During storage at different temperatures, bread containing xylanase exhibited less firmness but larger volume with whiter crumb color and longer shelf life as compared to control bread. Results of firmness, enthalpy, Fourier Transformation Infra Red (FTIR) and X-Ray Diffraction (XRD) studies suggested a lower staling rate of bread containing xylanase as compared to control one. Bread containing xylanase showed a smoother surface and more uniform pore size than the control. Significant differences in microstructure of control and bread containing xylanase were observed which might be attributed due to the change in water starch gluten interaction. These differences were also found to be interrelated to the textural properties of bread. Better sensory features were achieved in bread containing xylanase.

Keywords: Microstructure, Texture, Enzyme, Firmness, Bread

References(40)

[1]

U. Fernandez, Y. Vodovotz, P. Courtney, M. Pascall, Extended shelf life of soy bread using modified atmosphere packaging, J. Food Prot. 69 (3) (2006) 693-698.

[2]

A. Pandey, C.R. Socool, D. Mitichell, New developments in solid-state fermentation. I, Bioprocess and products, Proc. Biochem. 35 (2000) 1153-1169.

[3]

X. Qiang, C. YomgLie, W. QianBing, Health benefit application of functional oligosaccharides, Carbohydr. Polym. 77 (3) (2009) 435-441.

[4]

U. Schwab, L. Lauritzen, T. Tholstrup, et al., Effect of the amount and type of dietary fat on cardiometabolic risk factors and risk of developing type 2 diabetes, cardiovascular diseases, and cancer: a systematic review, Food Nutr. Res. 58 (2014) 1-26.

[5]
R. Ezekiel, N. Singh, Use of Potato Flour in Bread and Flat Bread, Impact of fibers on physical characteristics of fresh and staled bake off bread, Breadmaking (Second Edition). Improving Quality, 2011, pp. 247–259.
DOI
[6]

G. Kerch, J. Zicans, R.M. Meri, The effect of chitosan oligosaccharides on bread staling, J. Food Eng. 52 (2010) 491-495.

[7]

M.D. Shogren, Y. Pomeranz, K.F. Finney, Counteracting the deleterious effects of fiber in breadmaking, Cereal Chem. 58 (2) (1981) 142-144.

[8]

E. Haseborg, A. Himmelstein, Quality problems with high-fiber breads solved by use of hemicellulase enzymes, Cereal Food World 33 (5) (1988) 419-422.

[9]

Z. Kohajdova, J. Karovicova, S. Schmidt, Significance of emulsifiers and hydrocolloids in bakery industry, Acta Chim. Slovaca 2 (1) (2009) 46-61.

[10]

K. Hartikainen, K. Katina, Improving the quality of high-fibre breads. A volume in Woodhead Publishing Series in Food Sc, Technol. Nutr. (2012) 736-753.

[11]

H.A. Gaavilighi, M.H. Azizi, A.M. Barzegar, M.A. Ameri, Effect of selected hydrocolloids on bread staling as evaluated by DSC and XRD, J. Food Technol. 4 (3) (2006) 185-188.

[12]

Z. Jiang, X. Li, S. Yang, L. Li, S. Tan, Improvement of the bread making quality of wheat flour by the hyperthermophilic xylanase from Thermotoga maritima, Food Res. Int. 38 (2005) 37-43.

[13]

M.E. Steffolani, P.D. Ribotta, G.T. Pérez, A.E. Leon, Combinations of glucose oxidase, α-amylase and xylanase affect dough properties and bread quality, Int. J. Food Sci. Technol. 47 (3) (2012) 525-534.

[14]

J.K. Purhagen, M.E. Sjoo, A.C. Eliasson, Starch affecting anti-staling agents and their function in freestanding and pan-baked bread, Food Hydrocol. 25 (7) (2011) 1656-1666.

[15]

H.K. Leung, J.A. Magnuson, B.L. Bruinsma, Water binding of wheat flour doughs and breads as studied by deuteron relaxation, J. Food Sci. 48 (1983) 95-99.

[16]

M.S. Kim-Shin, F. Mari, P.A. Rao, T.R. Stengle, P. Chinachoti, 17O nuclear magnetic resonance studies of water mobility during bread staling, J. Agric. Food Chem. 39 (1991) 1915-1920.

[17]

E. Curti, E. Carini, G. Tribuzio, E. Vittadini, Bread staling: effect of gluten on physico-chemical properties and molecular mobility, LWT: Food Sci. Technol. 59 (1) (2014) 418-425.

[18]

A.A. Karim, M.H. Norzian, C.C. Seow, Methods of study of starch retrogradation, Food Chem. 71 (2000) 9-36.

[19]

D. Sievert, Z. Czuchajowska, Y. Pomeranz, Enzyme-resistant starch. III. X-ray diffraction of autoclaved amylomaize VII starch and enzyme-resistant starch residues, Cereal Chem. 68 (1) (1991) 86-91.

[20]
G. Ghoshal, Studies on the isolation, screening and characterization of a novel xylanase producing organism and its application in food processing, in: PhD Thesis, Panjab University, Chandigarh, India, 2011.
[21]

G. Ghoshal, U.C. Banerjee, U.S. Shivhare, Xylanase production by Penicillium citrinum in laboratory scale stirred tank reactor, Chem. Biochem. Eng. Q. 28 (3) (2014) 57-66.

[22]

G. Ghoshal, U.C. Banerjee, U.S. Shivhare, Isolation, screening and optimization of xylanase production in submerged production using P. citrinum, J. Sci. Ind. Res. 74 (2015) 400-405.

[23]
AACC, American Association of Cereal Chemists, Approved Methods of the AACC, 10th ed., The Association, St. Paul, MN, 2000.
[24]
AOAC, Official Methods of analysis, 17th ed., Association of Official Analytical Chemists, Arlington, VA, 2000.
[25]

G. Ghoshal, U.S. Shivhare, U.C. Banerjee, Effect of xylanase on quality attributes of whole wheat bread, J. Food Qual. 36 (2013) 172-180.

[26]

I. Demirkesen, B. Mert, G. Sumnu, S. Sahin, Rheological properties of gluten-free bread formulations, J. Food Eng. 96 (2010) 295-303.

[27]

S.K. Kim, B.L. D’Appolonia, Bread staling studies. II-Effect of protein content and storage temperature on the role of starch, Cereal Chem. 54 (1977) 216-224.

[28]

N.D.M. Villanueva, M.A.A.P. Da Silva, Comparative performance of the nine-point hedonic, hybrid and self-adjusting scales in the generation of internal preference maps, Food Qual. Preference 20 (1) (2009) 1-12.

[29]

R. Maheshwari, G. Bahadradwaj, M.K. Bhat, Thermophilic fungi: their physiology and enzymes, Microbiol. Mol. Biol. Rev. 64 (2000) 461-488.

[30]

R.J. Ridgwell, J.H. de Michieli, M. Fischer, et al., Xylanase induced changes to water and alkali extractable arabinoxylans in wheat flour: their role in lowering batter viscosity, J. Cereal Sci. 33 (2001) 83-96.

[31]

A.C. Norma, A.O. Guillermo, Production, purification and characterization of a low-molecular-mass xylanase from Aspergillus sp. and its application in baking, Appl. Biochem. Biotechnol. 104 (2003) 159-171.

[32]

P. Koletta, M. Irakli, M. Papageorgiou, et al., Physicochemical and technological properties of highly enriched wheat breads with wholegrain non wheat flours, J. Cereal Sci. 60 (3) (2014) 561-568.

[33]

J.H. Jagannath, K.S. Jayaraman, S.S. Arya, et al., Differential scanning calorimetry and wide-angle X-rayscattering studies of bread staling, J. Appl. Polm. Sci. 67 (1998) 1597-1603.

[34]

E. Armero, C. Collar, Crumb firming kinetics of wheat breads with anti-staling additives, J. Cereal Sci. 28 (2) (1998) 165-174.

[35]

J.A. Rojas, C.M. Rosell, C. Benedito de Barber, Role of maltodextrins in the staling of starch gels, Eur. Food Res.Technol. 212 (2001) 364-368.

[36]

A.C. Dona, G. Pages, G. Robert, R.G. Gilbert, P.W. Kuchel, Digestion of starch: in vivo and in vitro kinetic models used to characterise oligosaccharide or glucose release, Carbohydr. Polym. 80 (3) (2010) 599-617.

[37]

H. Goesaert, L. Slade, H. Levine, A. Jan, J.A. Delcour, Amylases and bread firming—an integrated view, J. Cereal Sci. 50 (3) (2009) 345-352.

[38]

M. Haros, C.M. Rosell, C. Benedito, Effect of different carbohydrases on fresh bread texture and bread staling, Eur. Food Res. Technol. 215 (2002) 425-430.

[39]

K.J. Zeleznak, R.C. Hoseney, Characterization of starch from bread aged at different temperatures, Starch 39 (1987) 231-233.

[40]

Z. Czuchajowska, Y. Pomeranz, DSC, water activity and moisture contents in crumb centre and near-crust zones of bread during storage, Cereal Chem. 66 (1989) 305-309.

Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 17 October 2015
Revised: 18 September 2016
Accepted: 23 September 2016
Published: 27 October 2016
Issue date: December 2016

Copyright

© 2016 Beijing Academy of Food Sciences.

Acknowledgements

Acknowledgements

G. Ghoshal and U.S. Shivhare are thankful to AICTE, New Delhi, India (Grant sanction no. 8023/BOR/RPS-2/2006-07; dated 26/02/2007) for providing financial support to carryout this work. Authors are also thankful to Prof. Tejbir Singh from Department of Chemistry, Panjab University, Chandigarh for his generous help in interpreting FTIR results.

Rights and permissions

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Return