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Food Science and Human Wellness 2015, 4(4): 162-168 https://doi.org/10.1016/j.fshw.2015.08.002
Research Article | Open Access | Issue | Published: 20 August 2015

Methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours inhibit some key enzymes linked to the pathology and complications of type 2 diabetes in vitro

Show Author's Information Emmanuel Anyachukwu Irondia,b( ),  Ganiyu Oboha,  Afolabi Akintunde Akindahunsia
Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, Akure 340001, Nigeria
Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University, Malete, P.M.B. 1530, Ilorin, Nigeria

Peer review under responsibility of Beijing Academy of Food Sciences.

Keywords:
Enzyme inhibition, Antioxidants, Type 2 diabetes, Phytochemical, Brachystegia eurycoma, Detarium microcarpum
Cite this article:
Irondi EA, Oboh G, Akindahunsi AA. Methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours inhibit some key enzymes linked to the pathology and complications of type 2 diabetes in vitro. Food Science and Human Wellness, 2015, 4(4): 162-168. https://doi.org/10.1016/j.fshw.2015.08.002
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Abstract Full text About this article

The inhibitory effect of methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours on some key enzymes [α-amylase, α-glucosidase and aldose reductase (AR)] linked to the pathology and complications of type 2 diabetes (T2D); and their antioxidant properties were evaluated. The antioxidant properties evaluated were DPPH• and ABTS•+ scavenging abilities, reducing power, and antioxidant phytochemicals (total phenolics, tannins, total flavonoids and total saponins). Extracts of both flours inhibited α-amylase, α-glucosidase and AR in a dose-dependent manner. The half-maximal inhibitory concentrations (IC50) of B. eurycoma on α-amylase, α-glucosidase, AR and lipid peroxidation were lower than those of D. microcarpum, indicating that it had stronger inhibitory potency than D. microcarpum. B. eurycoma also had significantly (P<0.05) higher DPPH• and ABTS•+ scavenging abilities, and reducing power than D. microcarpum. The antioxidant phytochemicals (total phenolics, tannins, total flavonoids and total saponins) were also significantly (P<0.05) higher in B. eurycoma than D. microcarpum. The inhibitory effect of B. eurycoma and D. microcarpum extracts on α-amylase, α-glucosidase and AR activities may be attributed to the combined action of their polyphenols and total saponins, and this may be a possible mechanism of action providing support for their use in managing hyperglycemia and the complications of T2D.


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About this article

Methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours inhibit some key enzymes linked to the pathology and complications of type 2 diabetes in vitro

Show Author's information Emmanuel Anyachukwu Irondia,b( ), Ganiyu Oboha, Afolabi Akintunde Akindahunsia
Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, Akure 340001, Nigeria
Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University, Malete, P.M.B. 1530, Ilorin, Nigeria

Peer review under responsibility of Beijing Academy of Food Sciences.

Abstract

The inhibitory effect of methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours on some key enzymes [α-amylase, α-glucosidase and aldose reductase (AR)] linked to the pathology and complications of type 2 diabetes (T2D); and their antioxidant properties were evaluated. The antioxidant properties evaluated were DPPH• and ABTS•+ scavenging abilities, reducing power, and antioxidant phytochemicals (total phenolics, tannins, total flavonoids and total saponins). Extracts of both flours inhibited α-amylase, α-glucosidase and AR in a dose-dependent manner. The half-maximal inhibitory concentrations (IC50) of B. eurycoma on α-amylase, α-glucosidase, AR and lipid peroxidation were lower than those of D. microcarpum, indicating that it had stronger inhibitory potency than D. microcarpum. B. eurycoma also had significantly (P<0.05) higher DPPH• and ABTS•+ scavenging abilities, and reducing power than D. microcarpum. The antioxidant phytochemicals (total phenolics, tannins, total flavonoids and total saponins) were also significantly (P<0.05) higher in B. eurycoma than D. microcarpum. The inhibitory effect of B. eurycoma and D. microcarpum extracts on α-amylase, α-glucosidase and AR activities may be attributed to the combined action of their polyphenols and total saponins, and this may be a possible mechanism of action providing support for their use in managing hyperglycemia and the complications of T2D.

Keywords: Enzyme inhibition, Antioxidants, Type 2 diabetes, Phytochemical, Brachystegia eurycoma, Detarium microcarpum

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Received: 31 January 2015
Revised: 10 August 2015
Accepted: 11 August 2015
Published: 20 August 2015
Issue date: December 2015

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