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Murraya paniculata (L.), a well-known medical plant, has widely been used to treat inflammation, stomach ache, internal and external injuries, and for other purposes. In this study, we determined the reducing, lipid peroxidation inhibition, 1,1-diphenyl-2-picryl-hydrazil radical (DPPH) scavenging, superoxide anion radical (O2−•) scavenging, hydroxyl radical (HO) scavenging and hydrogen peroxide (H2O2) scavenging activities of the methanolic extract of M. paniculata (MPE) by UV–vis spectrophotometer. The results showed that M. paniculata was rich in flavonoids (375 mg RE/g of extract). Reducing, lipid peroxidation inhibition and HO scavenging activities of the extract were 0.26 mg/mL, 0.023 mg/mL and 0.302 mg/mL, respectively, these activities were significantly higher than those of trolox. Other antioxidative behavior indicators, i.e., DPPH scavenging, O2−• scavenging and H2O2 scavenging activities of MPE were 0.93 mg/mL, 0.581 mg/mL and 0.47 mg/mL, respectively, and were comparable to those exhibited by trolox. These results indicate that the methanolic extract of M. paniculata exhibited strong antioxidative and radical scavenging activities.


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Studies on antioxidative activities of methanol extract from Murraya paniculata

Show Author's information Chao-hua ZhuZhen-lin LeiYan-ping Luo( )
Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Environment and Plant Protection, Hainan University, Haikou, Hainan 570228, PR China

Peer review under responsibility of Beijing Academy of Food Sciences.

Abstract

Murraya paniculata (L.), a well-known medical plant, has widely been used to treat inflammation, stomach ache, internal and external injuries, and for other purposes. In this study, we determined the reducing, lipid peroxidation inhibition, 1,1-diphenyl-2-picryl-hydrazil radical (DPPH) scavenging, superoxide anion radical (O2−•) scavenging, hydroxyl radical (HO) scavenging and hydrogen peroxide (H2O2) scavenging activities of the methanolic extract of M. paniculata (MPE) by UV–vis spectrophotometer. The results showed that M. paniculata was rich in flavonoids (375 mg RE/g of extract). Reducing, lipid peroxidation inhibition and HO scavenging activities of the extract were 0.26 mg/mL, 0.023 mg/mL and 0.302 mg/mL, respectively, these activities were significantly higher than those of trolox. Other antioxidative behavior indicators, i.e., DPPH scavenging, O2−• scavenging and H2O2 scavenging activities of MPE were 0.93 mg/mL, 0.581 mg/mL and 0.47 mg/mL, respectively, and were comparable to those exhibited by trolox. These results indicate that the methanolic extract of M. paniculata exhibited strong antioxidative and radical scavenging activities.

Keywords: Antioxidative activity, Flavonoids, Methanol extract, Murraya paniculata, Radical scavenging activities

References(36)

[1]

L.M. Perry, J. Metzger, Medicinal Plants of East and Southeast Asia, The MIT Press, Cambridge, 1980, pp. 367.

[2]

F. Imai, T. Kinoshita, U. Sankawa, Constituents of the leaves of Murraya paniculata collected in Taiwan, Chem. Pharm. Bull. 37 (1989) 358-362.

[3]

B.N. Sastri, The wealth of India. A dictionary of Indian raw materials and industrial products, Raw Mater. 6 (1956) 47-50.

[4]

A.U. Rahman, M. Shabbir, S.Z. Sultani, A. Jabbar, M.I. Choudhary, Cinnamates and coumarins from the leaves of Murraya paniculata, Phytochemistry 44 (1997) 683-685.

[5]

A. Ghani, Medicinal Plants of Bangladesh: Chemical constituents and Uses, Second ed., Asiatic Society of Bangladesh, Dhaka, 1998, pp. 309–310.

[6]

S.S.S.A. Aziz1, M.A. Sukari, M. Rahmani, M. Kitajima, N. Aimi, N.J. Ahpandi, Coumarins from Murraya paniculata (rutaceae), Malays. J. Anal. Sci. 14 (2010) 1-5.

[7]

C.H. Chen, H.C. Chan, Y.T. Chu, H.Y. Ho, P.Y. Chen, T.H. Lee, C.K. Lee, Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase, Molecules 14 (2009) 2947-2958.

[8]

M.K. Gautam, Anamika Gupta, C.V. Rao, R.K. Goel, Antihyperglycemic and antioxidant potential of Murraya paniculata linn. leaves: a preclinical study, J. Pharm. Res. 5 (2012) 1334-1337.

[9]

E.J. Rodríguez, G. Ramis-Ramos, Y.V. Heyden, E.F. Simó-Alfonso, M.J. Lerma-García, Y. Saucedo-Hernández, U. Monteagudo, Y. Morales, B. Holgado, J.M. Herrero-Martínez, Chemical composition, antioxidant properties and antimicrobial activity of the essential oil of Murraya paniculata leaves from the mountains of Central Cuba, Nat. Prod. Commun. 7 (2012) 1527-1530.

[10]

T.A. Mita, M.H. Shihan, M. Rahman, T. Sharmin, M. Maleque, M. Razi-Ul-Hasan Alvi, S.R. Chowdhury, In vitro antioxidant, cytotoxic, thrombolytic, antimicrobial and membrane stabilizing activities of Murraya paniculata, Am. J. Res. Commun. 1 (2013) 226-237.

[11]

N.O. Olawore, I.A. Ogunwander, O. Ekundayo, K.A. Adeleke, Chemical composition of the leaf and fruit essential oils of Murraya paniculata (L.) Jack. (Syn. Murraya exotica Linn.), Flavour Frag. J. 20 (2005) 54-56.

[12]

S. Saied, Studies in the Chemical Constituents of Murraya paniculata and ipomoea hederacea, departlvient of chemistry university of Karachi, Pakistan, 2005, pp. 5–16.

[13]

B.R.R. Rao, D.K. Rajput, G.R. Mallavarapu, Chemical diversity in curry leaf (Murraya koenigii) essential oils, Food Chem. 126 (2011) 989-994.

[14]

T. Kinoshita, K. Firman, Highly oxygenated flavonoids from Murraya paniculata, Phytochemistry 42 (1996) 1207-1210.

[15]

M.A. Sukari, M. Rahmani, N. Aimi, Polysubstituted flavonoid from the leaves of Murraya paniculata (Rutaceae), Nat. Prod. Sci. 9 (2003) 56-59.

[16]

J.Y. Zhang, N. Li, Y.Y. Che, Y. Zhang, S.X. Liang, M.B. Zhao, Y. Jiang, P.F. Tu, Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry, J. Pharm. Biomed. 56 (2011) 950-961.

[17]

J. Tchoumtchoua, D. Njamen, J.C. Mbanya, A. Skaltsounis, M. Halabalaki, Structure-oriented UHPLC-LTQ Orbitrap-based approach as a dereplication strategy for the identification of isoflavonoids from Amphimas pterocarpoides crude extract, J. Mass Spectrom. 48 (2013) 561-575.

[18]

S.B. Lotito, B. Frei, Relevance of apple polyphenolsas antioxidants in human plasma: contrasting in vitro and in vivo effects, Free Radic. Biol. Med. 36 (2004) 201-211.

[19]

L. Sun, J. Zhang, X. Lu, L. Zhang, Y. Zhang, Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leaves, Food Chem. Toxicol. 49 (2011) 2689-2696.

[20]

I. Gulcin, M.E. Buyukokuroglu, M. Oktay, O.I. Kufrevioglu, Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. Sub sp. pallsiana (Lamb.) Holmboe, J. Ethnopharmacol. 86 (2003) 51-58.

[21]

Y.C. Chung, S.J. Chen, C.K. Hsu, C.T. Chang, S.T. Chou, Studies on the antioxidative activity of Graptopetalum Paraguayense E. Walther, Food Chem. 91 (2005) 419-424.

[22]

R. Manian, N. Anusuya, P. Siddhuraju, S. Manian, The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficusbengalensis L. and Ficusracemosa L., Food Chem. 107 (2008) 1000-1007.

[23]

T. Osawa, M. Namiki, Natural antioxidant isolated from eucalyptus leaf waxes, J. Agric. Food Chem. 33 (1985) 777-780.

[24]

Y.C. Chung, C.T. Chang, W.W. Chao, C.F. Lin, S.T. Chou, Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NKI, J. Agric. Food Chem. 50 (2002) 2454-2458.

[25]

T. Osawa, M. Namiki, A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves, Agric. Biol. Chem. 45 (1981) 735-740.

[26]

I. Gulcin, Antioxidant and antiradical activities of l-carnitine, Life Sci. 78 (2006) 803-811.

[27]

A.L. Miller, Antioxidant flavonoids: structure, function and clinical usage, Alt. Med. Rev. 1 (1996) 103-111.

[28]

E. Vamanu, In vitro antimicrobial and antioxidant activities of ethanolic extract of lyophilized mycelium of Pleurotus ostreatus PQMZ91109, Molecules 17 (2012) 3653-3671.

[29]

M. Nishikimi, A. Rao, K. Yagi, The occurrence of superoxide anion in the reaction of reduced phenazinemethosulphate and molecular oxygen, Biochem. Biophys. Res. Commun. 46 (1972) 849-854.

[30]

K. Elizabeth, M.N.A. Rao, Oxygen radical scavenging activity of curcumin, Int. J. Pharm. 58 (1990) 237-240.

[31]

L.R.C. Barclay, S.J. Locke, J.M. MacNeil, Autoxidation in micelles. Synergism of vitamin C with lipid-soluble vitamin E and water-soluble Trolox, Can. J. Chem. 63 (1985) 366-374.

[32]

W. Fu, J. Chen, Y. Cai, Y. Lei, L. Chen, L. Pei, D. Zhou, X. Liang, J. Ruan, Antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica (Franch. et Sav.) Ching, J. Ethnopharmacol. 130 (2010) 521-528.

[33]

M. Elmastasa, O. Isildaka, I. Turkekulb, N. Temura, Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms, J. Food Compost. Anal. 20 (2007) 337-345.

[34]

J.Y. Yeh, L.H. Hsieh, K.T. Wu, C.F. Tsai, Antioxidant properties and antioxidant compounds of various extracts from the edible basidiomycete Grifola frondosa (Maitake), Molecules 16 (2011) 3197-3211.

[35]

P.G. Pietta, Flavonoids as antioxidants, J. Nat. Prod. 63 (2000) 1035-1042.

[36]

A.P. Wickens, Aging and the free radical theory, Respir. Physiol. 128 (2001) 379-391.

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Received: 05 May 2015
Revised: 25 June 2015
Accepted: 02 July 2015
Published: 09 July 2015
Issue date: September 2015

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© 2015 Beijing Academy of Food Sciences.

Acknowledgements

We are grateful for financial support from the National NSFC (No. 21162007) and Special Funds to Enhance the Comprehensive Strength of Midwestern Universities.

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