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Research paper | Open Access

SSR genetic linkage map construction of pea (Pisum sativum L.) based on Chinese native varieties

Xuelian Suna,b, Tao Yanga,b, Junjie Haoc, Xiaoyan Zhangc, Rebecca Fordd, Junye Jianga,b, Fang Wanga,b, Jianping Guana,b, Xuxiao Zonga,b( )
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Qingdao Academy of Agricultural Sciences, Qingdao 266100, China
Department of Agriculture and Food Systems, Melbourne School of Land and Environment, The University of Melbourne, Melbourne, Victoria 3010, Australia

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science, CAAS.

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Abstract

Simple sequence repeat (SSR) markers have previously been applied to linkage mapping of the pea (Pisum sativum L.) genome. However, the transferability of existing loci to the molecularly distinct Chinese winter pea gene pool was limited. A novel set of pea SSR markers was accordingly developed. Together with existing SSR sequences, the genome of the G0003973 (winter hardy)×G0005527 (cold sensitive) cross was mapped using 190 F2 individuals. In total, 157 SSR markers were placed in 11 linkage groups with an average interval of 9.7cM and total coverage of 1518cM. The novel markers and genetic linkage map will be useful for marker-assisted pea breeding.

Keywords

PeaSSR markersGenetic linkage mapMarker-assisted breeding

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The Crop Journal
Volume 2 Issue 2-3,
June 2014
Pages 170-174
DOI: 10.1016/j.cj.2014.03.004

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Cite this article:
Sun X, Yang T, Hao J, et al. SSR genetic linkage map construction of pea (Pisum sativum L.) based on Chinese native varieties. The Crop Journal, 2014, 2(2-3): 170-174. https://doi.org/10.1016/j.cj.2014.03.004

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Received: 08 November 2013
Revised: 22 February 2014
Accepted: 19 March 2014
Published: 27 March 2014
© 2014 Crop Science Society of China and Institute of Crop Sciences, CAAS.All rights reserved.