Effect of Sampling Frequency on a Unilateral Isometric Hamstring Strength Assessment Using Force Plates

The purpose of this study was to determine the effect of sampling frequency on the 90–90° (90-degrees hip and knee flexion) isometric hamstring assessment. Thirty-three elite female soccer players (age: 18.7 ± 3.7 years; height: 158.3 ± 5.9 cm; body mass: 62.8 ± 5.5 kg) performed three unilateral trials on a single occasion of the 90–90° isometric hamstring assessment. Force-time data were collected using force plates at 1000 Hz and down sampled to 500-, 250-, and 100 Hz. Peak force (N), force (N) at 100- and 200 ms and average rate of force development (aRFD) (N/s) over a 100- and 200 ms epoch were calculated. A repeated measures of analysis of variance and effect size was used to compare means. Excellent absolute and good relative reliability was observed for peak force across all sampling frequencies. Force at 100- and 200 ms and aRFD over 100 ms and 200 ms resulted poor-moderate relative reliability and poor-excellent absolute reliability. No significant trivial differences were observed for peak force between sampling frequencies (P > 0.05, Cohen’s d = 0.02–0.12). A significant difference (P < 0.001) was identified in 500, 250 and 100 Hz, with small-moderate and small-large increases in force at set time points and aRFD, respectively, in comparison to 1000 Hz (d = 0.21–2.00). Higher sampling frequencies (> 500 Hz) reduces the reliability of time dependent force characteristics, with minimal effect on peak force. Regular monitoring of peak force can be performed with higher sampling frequencies, but lower sampling frequencies would be beneficial to collect reliable rapid-force generating measures.