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Biophysics Reports 2019, 5(1): 10-18 https://doi.org/10.1007/s41048-018-0080-0
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Detection of cancer cells based on glycolytic-regulated surface electrical charges

Show Author's Information Wenjun Le1,  Bingdi Chen1,  Zheng Cui1,2,  Zhongmin Liu1( ),  Donglu Shi1,3( )
The Institute for Translational Nanomedicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai 200092, China
Department of Pathology, School of Medicine, Wake Forest University, Winston-Salem, NC, USA
Department of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH, USA
Keywords:
Nanotechnology, Cancer cell, Circulating tumor cells, Cell surface charge
Cite this article:
Le W, Chen B, Cui Z, et al. Detection of cancer cells based on glycolytic-regulated surface electrical charges. Biophysics Reports, 2019, 5(1): 10-18. https://doi.org/10.1007/s41048-018-0080-0
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Abstract Full text About this article

Over the past decades, cell surface charge, although experimentally observed, has not been well understood particularly from the viewpoint of biophysics. Our recent studies have shown that all cancer cells exhibit negative surface charges that are directly proportional to the secreted lactic acid, a unique cancer metabolic characteristic: high rate of glycolysis. We have therefore designed and developed a set of electrically-charged, fluorescent, and super-paramagnetic nanoprobes, capable of sensitive detection of cancer cells based on the surface charges. These probes are utilized to bind onto cells via electrostatic reaction for capture and magnetic separation. In this fashion, we are able to characterize cell surface charges that are regulated by different metabolic patterns, therefore effectively distinguishing the cancer cells from the normal cells. All 22 cancer cells of different organs are found to be negatively-charged therefore bound strongly by the positively-charged nanoprobes, whereas the normal cells show insignificant binding to the nanoprobes of either charge signs (positive or negative). This finding suggests that all tested cancer cells are negatively-charged and normal cells are either charge-neutral or slightly positive. For diagnosis, cancer cells can be detected, electrostatically bound, and magnetically separated in blood by charged and super-paramagnetic nanoprobes. In therapeutics, circulating cancer cells (CTCs) can be filtered and removed in a continuous fashion to reduce the risk of cancer metastasis. If successful, this new nanotechnology will revolutionize early cancer diagnosis and potentially enable new therapeutics in clinical settings.


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About this article

Detection of cancer cells based on glycolytic-regulated surface electrical charges

Show Author's information Wenjun Le1, Bingdi Chen1, Zheng Cui1,2, Zhongmin Liu1( ), Donglu Shi1,3( )
The Institute for Translational Nanomedicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai 200092, China
Department of Pathology, School of Medicine, Wake Forest University, Winston-Salem, NC, USA
Department of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH, USA

Abstract

Over the past decades, cell surface charge, although experimentally observed, has not been well understood particularly from the viewpoint of biophysics. Our recent studies have shown that all cancer cells exhibit negative surface charges that are directly proportional to the secreted lactic acid, a unique cancer metabolic characteristic: high rate of glycolysis. We have therefore designed and developed a set of electrically-charged, fluorescent, and super-paramagnetic nanoprobes, capable of sensitive detection of cancer cells based on the surface charges. These probes are utilized to bind onto cells via electrostatic reaction for capture and magnetic separation. In this fashion, we are able to characterize cell surface charges that are regulated by different metabolic patterns, therefore effectively distinguishing the cancer cells from the normal cells. All 22 cancer cells of different organs are found to be negatively-charged therefore bound strongly by the positively-charged nanoprobes, whereas the normal cells show insignificant binding to the nanoprobes of either charge signs (positive or negative). This finding suggests that all tested cancer cells are negatively-charged and normal cells are either charge-neutral or slightly positive. For diagnosis, cancer cells can be detected, electrostatically bound, and magnetically separated in blood by charged and super-paramagnetic nanoprobes. In therapeutics, circulating cancer cells (CTCs) can be filtered and removed in a continuous fashion to reduce the risk of cancer metastasis. If successful, this new nanotechnology will revolutionize early cancer diagnosis and potentially enable new therapeutics in clinical settings.

Keywords: Nanotechnology, Cancer cell, Circulating tumor cells, Cell surface charge

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Publication history
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Publication history

Received: 29 May 2018
Accepted: 27 August 2018
Published: 01 March 2019
Issue date: February 2019

Copyright

© The Author(s) 2019

Acknowledgements

Acknowledgement

This study was supported by National Natural Science Foundation of China (31671029, 81772285), Shanghai Natural Science Foundation (16ZR1400700), National Postdoctoral Program for Innovation Talents (BX201700173), and China Postdoctoral Science Foundation (2017M621534).

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