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Circulating microRNAs (miRNAs) play a pivotal role in the occurrence and development of acute myocardial infarction (AMI), and precise detection of them holds significant clinical implications. The development of luminol-based luminophores in the field of electrochemiluminescence (ECL) for miRNA detection has been significant, while their effectiveness is hindered by the instability of co-reactant hydrogen peroxide (H2O2). In this work, an iron single-atom catalyst (Fe-PNC) was employed for catalyzing the luminol-O2 ECL system to achieve ultra-sensitive detection of myocardial miRNA. Target miRNA triggers a hybridization chain reaction (HCR), resulting in the generation of a DNA product featuring multiple sticky ends that facilitate the attachment of Fe-PNC probes to the electrode surface. The Fe-PNC catalyst exhibits high promise and efficiency for the oxygen reduction reaction (ORR) in electrochemical energy conversion systems. The resulting ECL biosensor allowed ultrasensitive detection of myocardial miRNA with a low detection limit of 0.42 fM and a wide linear range from 1 fM to 1.0 nM. Additionally, it demonstrates exceptional performance when evaluated using serum samples collected from patients with AMI. This work expands the application of single-atom catalysis in ECL sensing and introduces novel perspectives for utilizing ECL in disease diagnosis.

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Publication history
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Acknowledgements

Publication history

Received: 13 March 2024
Revised: 29 April 2024
Accepted: 15 May 2024
Published: 06 June 2024

Copyright

© Tsinghua University Press 2024

Acknowledgements

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 22004003), the Natural Science Foundation of Anhui Province for Distinguished Young Scholars (No. 2008085J11), the Open Project Program of Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science (No. M2024-5), MOE, the Open Project of Engineering Research Center of Biofilm Water Purification and Utilization Technology of Ministry of Education (No. BWPU2023KF06), and the Natural Science Research Project of Anhui Province Education Department (No. 2023AH051116).

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