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Nano Research 2024, 17(5): 4270-4278 https://doi.org/10.1007/s12274-023-6277-7
Research Article | Issue | Published: 01 December 2023

Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment

Show Author's Information Xue Mao1,§,  Zhen-Zhen Li3,§,  Dong-Lian Hao1,  Wei-Dong Han4,  Gao-Peng Li2( ),  Yao-Yu Wang5,  Kun Zhang1( )
School of Textile Science and Engineering, Key Laboratory of Functional Textile Material and Product (Ministry of Education), Xi’an Polytechnic University, Xi’an 710048, China
Key Laboratory of Magnetic Molecules & Magnetic Information Materials of the Ministry of Education, School of Chemistry & Material Science, Shanxi Normal University, Taiyuan 030031, China
Department of Clinical Laboratory, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China
Shanghai iProteome Biotechnology Co., Ltd, Shanghai 201210, China
College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China

§ Xue Mao and Zhen-Zhen Li contributed equally to this work.

Keywords:
biomimetic materials, flexibility, inorganic nanofibers, flexible ceramic nanofibrous membranes, exceptional mechanical performance
Topics:
ElectrospinningNanofibersOxides
Cite this article:
Mao X, Li Z-Z, Hao D-L, et al. Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment. Nano Research, 2024, 17(5): 4270-4278. https://doi.org/10.1007/s12274-023-6277-7
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Abstract Full text Electronic supplementary material About this article

Developing free-standing and mechanical robust membrane materials capable of superior enrichment of phosphopeptides for analyzing and identifying the specific phosphoproteome of cancer cells is significant in understanding the molecular mechanisms of cancer development and exploring new therapeutic approaches, but still a significant challenge in materials design. To this end, we firstly constructed highly flexible ZrTiO4 nanofibrous membranes (NFMs) with excellent mechanical stability through a cost-effective and scalable electrospinning and subsequent calcination technique. Then, to further increase the enrichment capacity of the phosphopeptide, the biomimetic TiO2@ZrTiO4 NFMs with root hair or leaf like branch microstructure are developed by the hydrothermal post-synthetic modification of ZrTiO4 NFMs through growing unfurling TiO2 nanosheets onto the ZrTiO4 nanofibers. Importantly, remarkable flexibility and mechanical stability enable the resulting TiO2@ZrTiO4 NFMs excellent practicability, while the biomimetic microstructure allows it outstanding enrichment ability of the phosphopeptide and identification ability of the specific phosphoproteins in the digest of cervical cancer cells. Specifically, 6770 phosphopeptides can be enriched by TiO2@ZrTiO4 NFMs (2205 corresponding phosphoproteins can be identified), and the value is much higher than that of ZrTiO4 NFMs (6399 phosphopeptides and 2132 identified phosphoproteins) and commercial high-performance TiO2 particles (4525 phosphopeptides and 1811 identified phosphoproteins). These results demonstrate the super ability of TiO2@ZrTiO4 NFMs in phosphopeptide enrichment and great potential for exploring the pathogenesis of cancer.


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Abstract
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Electronic supplementary material
About this article

Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment

Show Author's information Xue Mao1,§, Zhen-Zhen Li3,§, Dong-Lian Hao1, Wei-Dong Han4, Gao-Peng Li2( ), Yao-Yu Wang5, Kun Zhang1( )
School of Textile Science and Engineering, Key Laboratory of Functional Textile Material and Product (Ministry of Education), Xi’an Polytechnic University, Xi’an 710048, China
Key Laboratory of Magnetic Molecules & Magnetic Information Materials of the Ministry of Education, School of Chemistry & Material Science, Shanxi Normal University, Taiyuan 030031, China
Department of Clinical Laboratory, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China
Shanghai iProteome Biotechnology Co., Ltd, Shanghai 201210, China
College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China

§ Xue Mao and Zhen-Zhen Li contributed equally to this work.

Abstract

Developing free-standing and mechanical robust membrane materials capable of superior enrichment of phosphopeptides for analyzing and identifying the specific phosphoproteome of cancer cells is significant in understanding the molecular mechanisms of cancer development and exploring new therapeutic approaches, but still a significant challenge in materials design. To this end, we firstly constructed highly flexible ZrTiO4 nanofibrous membranes (NFMs) with excellent mechanical stability through a cost-effective and scalable electrospinning and subsequent calcination technique. Then, to further increase the enrichment capacity of the phosphopeptide, the biomimetic TiO2@ZrTiO4 NFMs with root hair or leaf like branch microstructure are developed by the hydrothermal post-synthetic modification of ZrTiO4 NFMs through growing unfurling TiO2 nanosheets onto the ZrTiO4 nanofibers. Importantly, remarkable flexibility and mechanical stability enable the resulting TiO2@ZrTiO4 NFMs excellent practicability, while the biomimetic microstructure allows it outstanding enrichment ability of the phosphopeptide and identification ability of the specific phosphoproteins in the digest of cervical cancer cells. Specifically, 6770 phosphopeptides can be enriched by TiO2@ZrTiO4 NFMs (2205 corresponding phosphoproteins can be identified), and the value is much higher than that of ZrTiO4 NFMs (6399 phosphopeptides and 2132 identified phosphoproteins) and commercial high-performance TiO2 particles (4525 phosphopeptides and 1811 identified phosphoproteins). These results demonstrate the super ability of TiO2@ZrTiO4 NFMs in phosphopeptide enrichment and great potential for exploring the pathogenesis of cancer.

Keywords: biomimetic materials, flexibility, inorganic nanofibers, flexible ceramic nanofibrous membranes, exceptional mechanical performance

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Publication history
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Acknowledgements

Publication history

Received: 04 September 2023
Revised: 09 October 2023
Accepted: 18 October 2023
Published: 01 December 2023
Issue date: May 2024

Copyright

© Tsinghua University Press 2023

Acknowledgements

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 52202110 and 22201167), the Natural Science Foundation of Science and Technology Agency of Shanxi Province (No. 20210302124654), the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (No. 2021L259), the Innovation and Entrepreneurship Training Program for College students in Shanxi Province (No. 20220312), the Outstanding Young Talents of Shaanxi Universities (2019), the Scientific and Technological Plan Project of Xi’an (No. 21XJZZ0012), the Key Research and Development Program of Shaanxi Province of China (No. 2022SF-201), the Service Local Special Program of Education Department of Shaanxi Province (No. 23JC029), and the Scientific and Technological Plan Project of the Beilin District of Xi’an City (No. GX2206).

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