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Nano Research 2024, 17(3): 1785-1791 https://doi.org/10.1007/s12274-023-6012-4
Research Article | Issue | Published: 14 August 2023

Highly sensitive nanozyme strip: An effective tool for forensic material evidence identification

Show Author's Information Juanji Hong1,§,  Zhanjun Guo1,§,  Dihan Duan2,§,  Yi Zhang2,  Xin Chen3,  Yongjiu Li4,  Zheng Tu4,  Lei Feng4,  Lei Chen5,  Xiyun Yan5( ),  Lizeng Gao5( ),  Minmin Liang1( ),  Demin Duan1,3,5( )
Experimental Center of Advanced Materials, School of Materials Science & Engineering, Beijing Institute of Technology, Beijing 100081, China
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China
Chongqing kangjuquanhong biology science and technology Co., ltd, Chongqing 400026, China
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China
CAS Engineering Laboratory for Nanozyme, Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academic of Science, Beijing 100101, China

§ Juanji Hong, Zhanjun Guo, and Dihan Duan contributed equally to this work.

Keywords:
peroxidase-like activity, nanozyme strip, human hemoglobin, CoFe2O4 nanozyme, blood evidence
Topics:
Nano detectionNanoprobes
Cite this article:
Hong J, Guo Z, Duan D, et al. Highly sensitive nanozyme strip: An effective tool for forensic material evidence identification. Nano Research, 2024, 17(3): 1785-1791. https://doi.org/10.1007/s12274-023-6012-4
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Abstract Full text Electronic supplementary material About this article

During criminal case investigations, blood evidence tracing is critical for criminal investigation. However, the blood stains are often cleaned or covered up after the crime, resulting in trace residue and difficult tracking. Therefore, a highly sensitive and specific method for the rapid detection of human blood stains remains urgent. To solve this problem, we established a nanozyme-based strip for rapid detection of blood evidence with high sensitivity and specificity. To construct reliable nanozyme strips, we synthesized CoFe2O4 nanozymes with high peroxidase-like activity by scaling up to gram level, which can be supplied for six million tests, and conjugated antibody as a detection probe in nanozyme strip. The developed CoFe2O4 nanozyme strip can detect human hemoglobin (HGB) at a concentration as low as 1 ng/mL, which is 100 times lower than the commercially available colloidal gold strips (100 ng/mL). Moreover, this CoFe2O4 nanozyme strip showed high generality on 12 substrates and high specificity to human HGB among 13 animal blood samples. Finally, we applied the developed CoFe2O4 nanozyme strip to successfully detect blood stains in three real cases, where the current commercial colloidal gold strip failed to do. The results suggest that the CoFe2O4 nanozyme strip can be used as an effective on-scene detection method for human blood stains, and can further be used as a long-term preserved material evidence for traceability inquiry.


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Abstract
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Electronic supplementary material
About this article

Highly sensitive nanozyme strip: An effective tool for forensic material evidence identification

Show Author's information Juanji Hong1,§, Zhanjun Guo1,§, Dihan Duan2,§, Yi Zhang2, Xin Chen3, Yongjiu Li4, Zheng Tu4, Lei Feng4, Lei Chen5, Xiyun Yan5( ), Lizeng Gao5( ), Minmin Liang1( ), Demin Duan1,3,5( )
Experimental Center of Advanced Materials, School of Materials Science & Engineering, Beijing Institute of Technology, Beijing 100081, China
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China
Chongqing kangjuquanhong biology science and technology Co., ltd, Chongqing 400026, China
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China
CAS Engineering Laboratory for Nanozyme, Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academic of Science, Beijing 100101, China

§ Juanji Hong, Zhanjun Guo, and Dihan Duan contributed equally to this work.

Abstract

During criminal case investigations, blood evidence tracing is critical for criminal investigation. However, the blood stains are often cleaned or covered up after the crime, resulting in trace residue and difficult tracking. Therefore, a highly sensitive and specific method for the rapid detection of human blood stains remains urgent. To solve this problem, we established a nanozyme-based strip for rapid detection of blood evidence with high sensitivity and specificity. To construct reliable nanozyme strips, we synthesized CoFe2O4 nanozymes with high peroxidase-like activity by scaling up to gram level, which can be supplied for six million tests, and conjugated antibody as a detection probe in nanozyme strip. The developed CoFe2O4 nanozyme strip can detect human hemoglobin (HGB) at a concentration as low as 1 ng/mL, which is 100 times lower than the commercially available colloidal gold strips (100 ng/mL). Moreover, this CoFe2O4 nanozyme strip showed high generality on 12 substrates and high specificity to human HGB among 13 animal blood samples. Finally, we applied the developed CoFe2O4 nanozyme strip to successfully detect blood stains in three real cases, where the current commercial colloidal gold strip failed to do. The results suggest that the CoFe2O4 nanozyme strip can be used as an effective on-scene detection method for human blood stains, and can further be used as a long-term preserved material evidence for traceability inquiry.

Keywords: peroxidase-like activity, nanozyme strip, human hemoglobin, CoFe2O4 nanozyme, blood evidence

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Publication history
Copyright
Acknowledgements

Publication history

Received: 02 June 2023
Revised: 11 July 2023
Accepted: 14 July 2023
Published: 14 August 2023
Issue date: March 2024

Copyright

© Tsinghua University Press 2023

Acknowledgements

Acknowledgements

The authors gratefully acknowledge the support from the National Natural Science Foundation of China (No. 82072324), the National Key R&D Program of China (No. 2019YFA0709200), National Natural Science Foundation of China Foundation of Innovative Research Group grant (No. 22121003) and the Chongqing Special Key Project of Technological Innovation and Application Development (No. cstc2019jscx-gksbX0053).

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