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Nano Research 2024, 17(3): 1827-1836 https://doi.org/10.1007/s12274-023-5915-4
Research Article | Issue | Published: 26 July 2023

Rapid and sensitive detection of Epstein-Barr virus antibodies in nasopharyngeal carcinoma by chemiluminescence strips based on iron-porphyrin single atom nanozyme

Show Author's Information Daji Wang1,§( ),  Jie Wang1,3,§,  Dan Liu2,§,  Jiuyang He4,  Meiying Wang5,  Haibing Huang3,  Guohui Nie3,  Hui Ding3( ),  Xiyun Yan1,4( )
Nanozyme Synthesis Center, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Center for Drug Evaluation, National Medical Products Administration, Beijing 100022, China
Shenzhen Key Laboratory of nanozymes and Translational Cancer Research, Department of Otolaryngology, and Shenzhen Institute of Translational Medicine, Shenzhen Second People’s Hospital/the First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen 518035, China
CAS Engineering Laboratory for Nanozyme, Key Laboratory of Protein and Peptide Pharmaceutical, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Department of Rheumatology and Immunology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518055, China

§ Daji Wang, Jie Wang, and Dan Liu contributed equally to this work.

Keywords:
iron-porphyrin, chemiluminescence strips, antibody detection, single-atom nanozyme (SANzyme), Epstein-Barr virus (EBV), nasopharyngeal carcinoma (NPC)
Topics:
Nano detectionNanocatalysts
Cite this article:
Wang D, Wang J, Liu D, et al. Rapid and sensitive detection of Epstein-Barr virus antibodies in nasopharyngeal carcinoma by chemiluminescence strips based on iron-porphyrin single atom nanozyme. Nano Research, 2024, 17(3): 1827-1836. https://doi.org/10.1007/s12274-023-5915-4
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Abstract Full text Electronic supplementary material About this article

The correlation between Epstein-Barr virus (EBV) infection and nasopharyngeal carcinoma (NPC) risk has been extensively researched. The continual monitoring of EBV-IgAs provides a promising approach of NPC screening in its early stage. In this study, we successfully synthesized a single-atom nanozyme (SANzyme) through the application of iron-porphyrin based metal organic framework (MOF-FeP). The MOF-FeP possesses precisely-defined electronic and geometric structures that accurately mimic highly-evolved catalytic site of natural peroxidase. The peroxidase-like activity of MOF-FeP enables it to catalyze the chemiluminescence of luminol substrate. By integrating MOF-FeP into a traditional strip, we created a rapid and highly-sensitive evaluation tool for detecting EBV-IgAs. Importantly, the MOF-FeP strip enables the simultaneous detection of three EBV-IgAs, greatly improving the accuracy of EBV-associated NPC screening. The sensitivities of the MOF-FeP strip (75.56%–93.30%) surpass those of current enzyme-linked immunosorbent assay (ELISA) methods (64.44%–82.22%). This test takes only 16 min to perform as opposed to the customary 1–2 h required for standard ELISA. Additionally, the MOF-FeP strip is suitable for whole blood samples, thereby significantly simplifying the sample preparation and detection process. In conclusion, the MOF-FeP strip combines the simplicity of traditional strip with the high catalytic activity of SANzyme. Our innovative MOF-FeP strip offers a new point-of-care strategy for EBV-IgAs detection, which is expected to markedly facilitate early screening for EBV-associated diseases.


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Abstract
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Outline
Electronic supplementary material
About this article

Rapid and sensitive detection of Epstein-Barr virus antibodies in nasopharyngeal carcinoma by chemiluminescence strips based on iron-porphyrin single atom nanozyme

Show Author's information Daji Wang1,§( ), Jie Wang1,3,§, Dan Liu2,§, Jiuyang He4, Meiying Wang5, Haibing Huang3, Guohui Nie3, Hui Ding3( ), Xiyun Yan1,4( )
Nanozyme Synthesis Center, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Center for Drug Evaluation, National Medical Products Administration, Beijing 100022, China
Shenzhen Key Laboratory of nanozymes and Translational Cancer Research, Department of Otolaryngology, and Shenzhen Institute of Translational Medicine, Shenzhen Second People’s Hospital/the First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen 518035, China
CAS Engineering Laboratory for Nanozyme, Key Laboratory of Protein and Peptide Pharmaceutical, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Department of Rheumatology and Immunology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518055, China

§ Daji Wang, Jie Wang, and Dan Liu contributed equally to this work.

Abstract

The correlation between Epstein-Barr virus (EBV) infection and nasopharyngeal carcinoma (NPC) risk has been extensively researched. The continual monitoring of EBV-IgAs provides a promising approach of NPC screening in its early stage. In this study, we successfully synthesized a single-atom nanozyme (SANzyme) through the application of iron-porphyrin based metal organic framework (MOF-FeP). The MOF-FeP possesses precisely-defined electronic and geometric structures that accurately mimic highly-evolved catalytic site of natural peroxidase. The peroxidase-like activity of MOF-FeP enables it to catalyze the chemiluminescence of luminol substrate. By integrating MOF-FeP into a traditional strip, we created a rapid and highly-sensitive evaluation tool for detecting EBV-IgAs. Importantly, the MOF-FeP strip enables the simultaneous detection of three EBV-IgAs, greatly improving the accuracy of EBV-associated NPC screening. The sensitivities of the MOF-FeP strip (75.56%–93.30%) surpass those of current enzyme-linked immunosorbent assay (ELISA) methods (64.44%–82.22%). This test takes only 16 min to perform as opposed to the customary 1–2 h required for standard ELISA. Additionally, the MOF-FeP strip is suitable for whole blood samples, thereby significantly simplifying the sample preparation and detection process. In conclusion, the MOF-FeP strip combines the simplicity of traditional strip with the high catalytic activity of SANzyme. Our innovative MOF-FeP strip offers a new point-of-care strategy for EBV-IgAs detection, which is expected to markedly facilitate early screening for EBV-associated diseases.

Keywords: iron-porphyrin, chemiluminescence strips, antibody detection, single-atom nanozyme (SANzyme), Epstein-Barr virus (EBV), nasopharyngeal carcinoma (NPC)

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Publication history
Copyright
Acknowledgements

Publication history

Received: 18 April 2023
Revised: 30 May 2023
Accepted: 11 June 2023
Published: 26 July 2023
Issue date: March 2024

Copyright

© Tsinghua University Press 2023

Acknowledgements

Acknowledgements

We thank Prof. Zhe Zhang from First Affiliated Hospital of Guangxi Medical University for his guidance on this study. This work was financially supported by the National Key Research and Development Program of China (No. 2019YFA0709204), the National Natural Science Foundation of China (No. 32200744), the Natural Science Foundation of Guangdong Province (Nos. 2021A1515110028 and 2022A1515220147), the Science and Technology Program for Basic Research in Shenzhen (Nos. JCYJ20210324103015039, JCYJ20190809095811254, and JCYJ20200109140412476), and the National Natural Science Foundation of China (Nos. 81930050 and 22121003).

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