Journal Home > Volume 16 , Issue 7
Nano Research 2023, 16(7): 9663-9671 https://doi.org/10.1007/s12274-023-5519-z
Research Article | Issue | Published: 13 April 2023

β-Cyclodextrin-modified AuBi metallic aerogels enable efficient peroxidase mimicking for colorimetric sensing of urease-positive pathogenic bacteria

Show Author's Information Minghui Wang,  Peixian Wu,  Sha Yang,  Gui-long Wu,  Na Li,  Xiaofeng Tan( ),  Qinglai Yang( )
Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang 421001, China
Keywords:
surface modification, metallic aerogels, urease activity, proteus mirabilis, bacteria detection, urea hydrolysis, fluoride ion
Topics:
Bacteria Nanosensors
Cite this article:
Wang M, Wu P, Yang S, et al. β-Cyclodextrin-modified AuBi metallic aerogels enable efficient peroxidase mimicking for colorimetric sensing of urease-positive pathogenic bacteria. Nano Research, 2023, 16(7): 9663-9671. https://doi.org/10.1007/s12274-023-5519-z
Download citation
EndNote(RIS)
BibTeX

1508

Views

125

Downloads

Citations

1

Crossref

0

WoS

2

Scopus

0

CSCD

Abstract Full text Electronic supplementary material About this article

The detection of pathogenic bacteria with improved accessibility, reduced analysis time, and increased sensitivity is of great importance for diagnosing the infected disease. Nanozymes have attracted rising attention in the bioassay field. Designing a model nanozyme needs the combined merit of sensible nanostructures and a large specific surface area to guarantee exceptional enzyme-mimic activity. Herein, a β-cyclodextrin modified AuBi aerogel is prepared by a one-pot reduction strategy. The introduction of β-cyclodextrin (featured with a hydrophobic cavity and hydrophilic surface) enhances the catalytic activity of AuBi aerogels by engendering host–guest complex and improving dispersity/stability. Based on the specific urea hydrolysis, which could produce NH3 to raise pH by urease, the pH up-regulation would inhibit the peroxidase-mimicking performances of β-cyclodextrin/AuBi aerogels. Therefore, the sensitive colorimetric detection platform for urease activity could be constructed. Moreover, the sensing platform can detect straightforwardly urease-positive Proteus mirabilis in urine circumstances with a wide detection range and a low limit of detection (LOD) of 4 colony-forming unit (CFU)·mL−1. The reproducibility, stability, and specificity of this approach are verified to be satisfactory. Also, as an inhibitor of urease activity, the fluoride ion could be detected by the constructed sensing platform sensitively and specifically. Overall, this work provides a blueprint for designing an ideal nanozyme and paves a new roadway for detecting pathogenic bacteria.


menu
Abstract
Full text
Outline
Electronic supplementary material
About this article

β-Cyclodextrin-modified AuBi metallic aerogels enable efficient peroxidase mimicking for colorimetric sensing of urease-positive pathogenic bacteria

Show Author's information Minghui Wang, Peixian Wu, Sha Yang, Gui-long Wu, Na Li, Xiaofeng Tan( ), Qinglai Yang( )
Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang 421001, China

Abstract

The detection of pathogenic bacteria with improved accessibility, reduced analysis time, and increased sensitivity is of great importance for diagnosing the infected disease. Nanozymes have attracted rising attention in the bioassay field. Designing a model nanozyme needs the combined merit of sensible nanostructures and a large specific surface area to guarantee exceptional enzyme-mimic activity. Herein, a β-cyclodextrin modified AuBi aerogel is prepared by a one-pot reduction strategy. The introduction of β-cyclodextrin (featured with a hydrophobic cavity and hydrophilic surface) enhances the catalytic activity of AuBi aerogels by engendering host–guest complex and improving dispersity/stability. Based on the specific urea hydrolysis, which could produce NH3 to raise pH by urease, the pH up-regulation would inhibit the peroxidase-mimicking performances of β-cyclodextrin/AuBi aerogels. Therefore, the sensitive colorimetric detection platform for urease activity could be constructed. Moreover, the sensing platform can detect straightforwardly urease-positive Proteus mirabilis in urine circumstances with a wide detection range and a low limit of detection (LOD) of 4 colony-forming unit (CFU)·mL−1. The reproducibility, stability, and specificity of this approach are verified to be satisfactory. Also, as an inhibitor of urease activity, the fluoride ion could be detected by the constructed sensing platform sensitively and specifically. Overall, this work provides a blueprint for designing an ideal nanozyme and paves a new roadway for detecting pathogenic bacteria.

Keywords: surface modification, metallic aerogels, urease activity, proteus mirabilis, bacteria detection, urea hydrolysis, fluoride ion

References(45)

[1]

Rippa, M.; Castagna, R.; Pannico, M.; Musto, P.; Borriello, G.; Paradiso, R.; Galiero, G.; Bolletti Censi, S.; Zhou, J.; Zyss, J. et al. Octupolar metastructures for a highly sensitive, rapid, and reproducible phage-based detection of bacterial pathogens by surface-enhanced Raman scattering. ACS Sens. 2017, 2, 947–954.
DOI Google Scholar
[2]

Wang, J. C.; Chi, S. W.; Yang, T. H.; Chuang, H. S. Label-free monitoring of microorganisms and their responses to antibiotics based on self-powered microbead sensors. ACS Sens. 2018, 3, 2182–2190.
DOI Google Scholar
[3]

Hameed, S.; Xie, L. J.; Ying, Y. B. Conventional and emerging detection techniques for pathogenic bacteria in food science: A review. Trends Food Sci. Technol. 2018, 81, 61–73.
DOI Google Scholar
[4]

Al Ramahi, R.; Zaid, A. N.; Abu-Khalaf, N. Evaluating the potential use of electronic tongue in early identification and diagnosis of bacterial infections. Infect. Drug Resist. 2019, 12, 2445–2451.
DOI Google Scholar
[5]

Majdinasab, M.; Hayat, A.; Marty, J. L. Aptamer-based assays and aptasensors for detection of pathogenic bacteria in food samples. TrAC Trends Anal. Chem. 2018, 107, 60–77.
DOI Google Scholar
[6]

Laliwala, A.; Svechkarev, D.; Sadykov, M. R.; Endres, J.; Bayles, K. W.; Mohs, A. M. Simpler procedure and improved performance for pathogenic bacteria analysis with a paper-based ratiometric fluorescent sensor array. Anal. Chem. 2022, 94, 2615–2624.
DOI Google Scholar
[7]

Santopolo, G.; Clemente, A.; Rojo-Molinero, E.; Oliver, A.; Noé, C. de la Rica, R. Rapid identification and classification of pathogens that produce carbapenemases and cephalosporinases with a colorimetric paper-based multisensor. Anal. Chem. 2022, 94, 9442–9449.
DOI Google Scholar
[8]
Zhou, J.; Tian, F. Y.; Fu, R. J.; Yang, Y. J.; Jiao, B. N.; He, Y. Enzyme-nanozyme cascade reaction-mediated etching of gold nanorods for the detection of Escherichia coli. ACS Appl. Nano Mater. 2020, 3, 9016–9025.
[9]
Eissa, S.; Zourob, M. Ultrasensitive peptide-based multiplexed electrochemical biosensor for the simultaneous detection of Listeria monocytogenes and Staphylococcus aureus. Microchim. Acta 2020, 187, 486.
[10]

Zhao, L. L.; Wiebe, J.; Zahoor, R.; Slavkovic, S.; Malile, B.; Johnson, P. E.; Chen, J. I. L. Colorimetric detection of catalase and catalase-positive bacteria (E. coli) using silver nanoprisms. Anal. Methods 2016, 8, 6625–6630.
DOI Google Scholar
[11]

Chen, P. P.; Wang, Y.; Meng, Y. M.; He, Y. Q.; Xiong, Y.; Ao, K. P.; Huang, J.; Chen, J.; Xie, Y.; Ying, B. W. Color and distance two-dimensional visual and homogeneous dual fluorescence analysis of pathogenic bacteria in clinical samples. Sens. Actuators B:Chem. 2022, 357, 131422.
DOI Google Scholar
[12]

Sun, Z. W.; Peng, Y.; Wang, M. H.; Lin, Y. X.; Jalalah, M.; Alsareii, S. A.; Harraz, F. A.; Yang, J.; Li, G. X. Electrochemical deposition of Cu metal-organic framework films for the dual analysis of pathogens. Anal. Chem. 2021, 93, 8994–9001.
DOI Google Scholar
[13]

Bishai, W. R.; Timmins, G. S. Potential for breath test diagnosis of urease positive pathogens in lung infections. J. Breath Res. 2019, 13, 032002.
DOI Google Scholar
[14]

Gong, Z. L.; Shi, X. L.; Bai, F.; He, X. L.; Zhang, H. Y.; Li, Y. B.; Wan, Y.; Lin, Y. M.; Qiu, Y. Q.; Chen, Q. C. et al. Characterization of a novel diarrheagenic strain of Proteus mirabilis associated with food poisoning in China. Front. Microbiol. 2019, 10, 2810.
DOI Google Scholar
[15]

Hu, W. C.; Pang, J.; Biswas, S.; Wang, K.; Wang, C.; Xia, X. H. Ultrasensitive detection of bacteria using a 2D MOF nanozyme-amplified electrochemical detector. Anal. Chem. 2021, 93, 8544–8552.
DOI Google Scholar
[16]

Hao, Z.; Lin, X. D.; Li, J. J.; Yin, Y. L.; Gao, X.; Wang, S.; Liu, Y. Q. Multifunctional nanoplatform for dual-mode sensitive detection of pathogenic bacteria and the real-time bacteria inactivation. Biosens. Bioelectron. 2021, 173, 112789.
DOI Google Scholar
[17]

Yang, E. L.; Li, D.; Yin, P. K.; Xie, Q. Y.; Li, Y.; Lin, Q. Y.; Duan, Y. X. A novel surface-enhanced Raman scattering (SERS) strategy for ultrasensitive detection of bacteria based on three-dimensional (3D) DNA walker. Biosens. Bioelectron. 2021, 172, 112758.
DOI Google Scholar
[18]

Robby, A. I.; Kim, S. G.; Lee, U. H.; In, I.; Lee, G.; Park, S. Y. Wireless electrochemical and luminescent detection of bacteria based on surface-coated CsWO3-immobilized fluorescent carbon dots with photothermal ablation of bacteria. Chem. Eng. J. 2021, 403, 126351.
DOI Google Scholar
[19]
Yao, W. Y.; Shi, J.; Ling, J.; Guo, Y. D.; Ding, C. S.; Ding, Y. J. SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilis. Microchim. Acta 2020, 187, 406.
[20]

Santopolo, G.; Doménech-Sánchez, A.; Russell, S. M. de la Rica, R. Ultrafast and ultrasensitive naked-eye detection of urease-positive bacteria with plasmonic nanosensors. ACS Sens. 2019, 4, 961–967.
DOI Google Scholar
[21]

Li, Q. L.; Li, H.; Li, K. X.; Gu, Y.; Wang, Y. J.; Yang, D. Z.; Yang, Y. L.; Gao, L. Specific colorimetric detection of methylmercury based on peroxidase-like activity regulation of carbon dots/Au NPs nanozyme. J. Hazard. Mater. 2023, 441, 129919.
DOI Google Scholar
[22]

Liu, P.; Wang, Y. B.; Han, L.; Cai, Y. Y.; Ren, H.; Ma, T. X.; Li, X. Q.; Petrenko, V. A.; Liu, A. H. Colorimetric assay of bacterial pathogens based on Co3O4 magnetic nanozymes conjugated with specific fusion phage proteins and magnetophoretic chromatography. ACS Appl. Mater. Interfaces 2020, 12, 9090–9097.
DOI Google Scholar
[23]
Xue, L.; Jin, N. N.; Guo, R. Y.; Wang, S. Y.; Qi, W. Z.; Liu, Y. J.; Li, Y. B.; Lin, J. H. Microfluidic colorimetric biosensors based on Mno2 nanozymes and convergence-divergence spiral micromixers for rapid and sensitive detection of Salmonella. ACS Sens. 2021, 6, 2883–2892.
[24]

Su, Y. T.; Wu, F.; Song, Q. X.; Wu, M. J.; Mohammadniaei, M.; Zhang, T. W.; Liu, B. L.; Wu, S. S.; Zhang, M.; Li, A. et al. Dual enzyme-mimic nanozyme based on single-atom construction strategy for photothermal-augmented nanocatalytic therapy in the second near-infrared biowindow. Biomaterials 2022, 281, 121325.
DOI Google Scholar
[25]

Wang, X. W.; Shi, Q. Q.; Zha, Z.; Zhu, D. D.; Zheng, L. R.; Shi, L. X.; Wei, X. W.; Lian, L.; Wu, K. L.; Cheng, L. Copper single-atom catalysts with photothermal performance and enhanced nanozyme activity for bacteria-infected wound therapy. Bioact. Mater. 2021, 6, 4389–4401.
DOI Google Scholar
[26]

Wei, H.; Gao, L. Z.; Fan, K. L.; Liu, J. W.; He, J. Y.; Qu, X. G.; Dong, S. J.; Wang, E. K.; Yan, X. Y. Nanozymes: A clear definition with fuzzy edges. Nano Today 2021, 40, 101269.
DOI Google Scholar
[27]

Zhang, R. F.; Yan, X. Y.; Fan, K. L. Nanozymes inspired by natural enzymes. Acc. Mater. Res. 2021, 2, 534–547.
DOI Google Scholar
[28]

Tang, G. H.; He, J. Y.; Liu, J. W.; Yan, X. Y.; Fan, K. L. Nanozyme for tumor therapy: Surface modification matters. Exploration 2021, 1, 75–89.
DOI Google Scholar
[29]

Zeng, Y. T.; Li, Y.; Tan, X. F.; Gong, J. D.; Wang, Z. Y.; An, Y. H.; Wang, Z. Q.; Li, H. B, N-doped PdRu aerogels as high-performance peroxidase mimics for sensitive detection of glucose. ACS Appl. Mater. Interfaces 2021, 13, 36816–36823.
DOI Google Scholar
[30]

Wang, H. J.; Fang, Q.; Gu, W. L.; Du, D.; Lin, Y. H.; Zhu, C. Z. Noble metal aerogels. ACS Appl. Mater. Interfaces 2020, 12, 52234–52250.
DOI Google Scholar
[31]

Xing, L.; Zheng, X. Y.; Tang, Y. L.; Zhou, X. M.; Hao, J. K.; Hu, L.; Shen, J. L.; Yan, Z. Q. Ag-β-cyclodextrin-graphene oxide ternary nanostructures with peroxidase-mimicking activity for Hg2+ detection. ACS Appl. Nano Mater. 2021, 4, 13807–13817.
DOI Google Scholar
[32]

Lu, W. H.; Zhang, J. X.; Li, N. L.; You, Z.; Feng, Z. Y.; Natarajan, V.; Chen, J.; Zhan, J. H. Co3O4@β-cyclodextrin with synergistic peroxidase-mimicking performance as a signal magnification approach for colorimetric determination of ascorbic acid. Sens. Actuators B:Chem. 2020, 303, 127106.
DOI Google Scholar
[33]

Ma, Y.; Jiang, K.; Chen, H. R.; Shi, Q. Q.; Liu, H.; Zhong, X. Y.; Qian, H. S.; Chen, X. L.; Cheng, L.; Wang, X. W. Liquid exfoliation of V8C7 nanodots as peroxidase-like nanozymes for photothermal-catalytic synergistic antibacterial treatment. Acta Biomater. 2022, 149, 359–372.
DOI Google Scholar
[34]

Xiao, L. P.; Zhu, A. M.; Xu, Q. C.; Chen, Y.; Xu, J.; Weng, J. Colorimetric biosensor for detection of cancer biomarker by Au nanoparticle-decorated Bi2Se3 nanosheets. ACS Appl. Mater. Interfaces 2017, 9, 6931–6940.
DOI Google Scholar
[35]

Wan, K.; Tan, A. D.; Yu, Z. P.; Liang, Z. X.; Piao, J. H.; Tsiakaras, P. 2D nitrogen-doped hierarchically porous carbon: Key role of low dimensional structure in favoring electrocatalysis and mass transfer for oxygen reduction reaction. Appl. Catal. B:Environ. 2017, 209, 447–454.
DOI Google Scholar
[36]

Wu, K. L.; Zhu, D. D.; Dai, X. L.; Wang, W. N.; Zhong, X. Y.; Fang, Z. B.; Peng, C.; Wei, X. W.; Qian, H. S.; Chen, X. L. et al. Bimetallic oxide Cu1.5Mn1.5O4 cage-like frame nanospheres with triple enzyme-like activities for bacterial-infected wound therapy. Nano Today 2022, 43, 101380.
DOI Google Scholar
[37]
Hong, C. Y.; Chen, L. L.; Huang, J. Y.; Shen, Y. L.; Yang, H. F.; Huang, Z. Y.; Cai, R.; Tan, W. H. Gold nanoparticle-decorated MoSe2 nanosheets as highly effective peroxidase-like nanozymes for total antioxidant capacity assay. Nano Res., in press, https://doi.org/10.1007/s12274-022-5328-9.
[38]

Xu, R. X.; Tan, X. F.; Li, T.; Liu, S. Q.; Li, Y.; Li, H. Norepinephrine-induced AuPd aerogels with peroxidase- and glucose oxidase-like activity for colorimetric determination of glucose. Microchim. Acta 2021, 188, 362.
DOI Google Scholar
[39]

Hong, C. Y.; Chen, L. L.; Wu, C. Y.; Yang, D.; Dai, J. Y.; Huang, Z. Y.; Cai, R.; Tan, W. H. Green synthesis of Au@WSe2 hybrid nanostructures with the enhanced peroxidase-like activity for sensitive colorimetric detection of glucose. Nano Res. 2022, 15, 1587–1592.
DOI Google Scholar
[40]

Xu, J.; Sun, F. Y.; Li, Q.; Yuan, H. X.; Ma, F. Y.; Wen, D.; Shang, L. Ultrasmall gold nanoclusters-enabled fabrication of ultrafine gold aerogels as novel self-supported nanozymes. Small 2022, 18, 2200525.
DOI Google Scholar
[41]

Jannah, F.; Kim, J. M. Ph-sensitive colorimetric polydiacetylene vesicles for urease sensing. Dyes Pigm. 2019, 169, 15–21.
DOI Google Scholar
[42]

Kashyap, S. J.; Sankannavar, R.; Madhu, G. M. Fluoride sources, toxicity and fluorosis management techniques—A brief review. J. Hazard. Mater. Lett. 2021, 2, 100033.
DOI Google Scholar
[43]

Zhou, Y.; Zhang, J. F.; Yoon, J. Fluorescence and colorimetric chemosensors for fluoride-ion detection. Chem. Rev. 2014, 114, 5511–5571.
DOI Google Scholar
[44]

Mazzei, L.; Cianci, M.; Benini, S.; Ciurli, S. The structure of the elusive urease-urea complex unveils the mechanism of a paradigmatic nickel-dependent enzyme. Angew. Chem., Int. Ed. 2019, 58, 7415–7419.
DOI Google Scholar
[45]

Zhang, Y.; Jiao, L.; Xu, W. Q.; Chen, Y. F.; Wu, Y.; Yan, H. Y.; Gu, W. L.; Zhu, C. Z. Defect-rich and ultrathin nitrogen-doped carbon nanosheets with enhanced peroxidase-like activity for the detection of urease activity and fluoride ion. Chin. Chem. Lett. 2022, 33, 1317–1320.
DOI Google Scholar
File
12274_2023_5519_MOESM1_ESM.pdf (1.5 MB)
Publication history
Copyright
Acknowledgements

Publication history

Received: 30 November 2022
Revised: 07 January 2023
Accepted: 18 January 2023
Published: 13 April 2023
Issue date: July 2023

Copyright

© Tsinghua University Press 2023

Acknowledgements

Acknowledgements

This work was financially supported by the Science and Technology Innovation Program of Hunan Province “Huxiang Young Talents Plan” (No. 2021RC3106), Hunan Provincial Natural Science Foundation of China (No. 2022JJ40360), and the Key Research and Development Program of Hunan Province, China (No. 2022SK2053). The authors would also like to thank Shiyanjia Lab (www.shiyanjia.com) for the support of the XPS and XRD tests.

Return