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Nano Research 2023, 16(5): 7383-7392 https://doi.org/10.1007/s12274-023-5460-1
Research Article | Issue | Published: 22 February 2023

Fish scale-derived scaffolds with MSCs loading for photothermal therapy of bone defect

Show Author's Information Siyu Shen1,2,  Rui Liu3,  Chuanhui Song3,  Tao Shen1,2,  Yiwen Zhou4,  Junxia Guo5,  Bin Kong6( ),  Qing Jiang1,2( )
State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Branch of National Clinical Research Center for Orthopedics, Sports Medicine and Rehabilitation, Nanjing 210008, China
The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing 210008, China
Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China
Keywords:
bone regeneration, photothermal therapy, mesenchymal stem cell, fish scale, GelMA
Topics:
Tissue engineering
Cite this article:
Shen S, Liu R, Song C, et al. Fish scale-derived scaffolds with MSCs loading for photothermal therapy of bone defect. Nano Research, 2023, 16(5): 7383-7392. https://doi.org/10.1007/s12274-023-5460-1
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Abstract Full text Electronic supplementary material About this article

Tissue engineering scaffolds have presented effective value in bone repair. However, the integration of the diverse components, complex structures, and multifunction to impart the scaffolds with improved applicability is still a challenge. Here, we propose a novel fish-derived scaffold combined with photothermal therapy and mesenchymal stem cells (MSCs) to promote bone regeneration. The fish-derived scaffold is composed of the decellularized fish scale and gelatin methacrylate synthesized from fish gelatin (fGelMA), which can promote the proliferation and osteogenesis of MSCs with no obvious immunological rejection. Furthermore, the black phosphorus (BP) nanosheets are incorporated into the fGelMA hydrogel network, which can endow the hydrogel with the capacity of photothermal conversion stimulated by near-infrared (NIR) light. The fish-derived scaffold can promote the osteogenesis process of MSCs with higher expression of osteogenic markers and higher mineralization assisted by the NIR light in vitro. The regeneration of mice calvarial defect has also been accelerated by the scaffold with photothermal therapy and MSCs. These results suggest that the fish-derived scaffold, photothermal therapy, and MSCs-based regenerative therapy is a promising clinical strategy in bone regeneration.


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Electronic supplementary material
About this article

Fish scale-derived scaffolds with MSCs loading for photothermal therapy of bone defect

Show Author's information Siyu Shen1,2, Rui Liu3, Chuanhui Song3, Tao Shen1,2, Yiwen Zhou4, Junxia Guo5, Bin Kong6( ), Qing Jiang1,2( )
State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
Branch of National Clinical Research Center for Orthopedics, Sports Medicine and Rehabilitation, Nanjing 210008, China
The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing 210008, China
Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China

Abstract

Tissue engineering scaffolds have presented effective value in bone repair. However, the integration of the diverse components, complex structures, and multifunction to impart the scaffolds with improved applicability is still a challenge. Here, we propose a novel fish-derived scaffold combined with photothermal therapy and mesenchymal stem cells (MSCs) to promote bone regeneration. The fish-derived scaffold is composed of the decellularized fish scale and gelatin methacrylate synthesized from fish gelatin (fGelMA), which can promote the proliferation and osteogenesis of MSCs with no obvious immunological rejection. Furthermore, the black phosphorus (BP) nanosheets are incorporated into the fGelMA hydrogel network, which can endow the hydrogel with the capacity of photothermal conversion stimulated by near-infrared (NIR) light. The fish-derived scaffold can promote the osteogenesis process of MSCs with higher expression of osteogenic markers and higher mineralization assisted by the NIR light in vitro. The regeneration of mice calvarial defect has also been accelerated by the scaffold with photothermal therapy and MSCs. These results suggest that the fish-derived scaffold, photothermal therapy, and MSCs-based regenerative therapy is a promising clinical strategy in bone regeneration.

Keywords: bone regeneration, photothermal therapy, mesenchymal stem cell, fish scale, GelMA

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Publication history
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Acknowledgements

Publication history

Received: 09 October 2022
Revised: 24 December 2022
Accepted: 01 January 2023
Published: 22 February 2023
Issue date: May 2023

Copyright

© Tsinghua University Press 2023

Acknowledgements

Acknowledgements

This work was supported by the National Key Research and Development Project (No. 2021YFA1201404), Key Program of National Natural Science Foundation of China (No. 81730067), Major Project of National Natural Science Foundation of China (No. 81991514), the National Natural Science Foundation of China (No. 82101184), Shenzhen Fundamental Research Program (No. JCYJ20210324102809024), Shenzhen PhD Start-up Program (No. RCBS20210609103713045), Jiangsu Provincial Key Medical Center Foundation, Jiangsu Provincial Medical Outstanding Talent Foundation, Jiangsu Provincial Medical Youth Talent Foundation, Jiangsu Provincial Key Medical Talent Foundation, and the Fundamental Research Funds for the Central Universities (Nos. 14380493 and 14380494).

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