1160
Views
11
Crossref
10
WoS
12
Scopus
0
CSCD
Tissue engineering scaffolds have presented effective value in bone repair. However, the integration of the diverse components, complex structures, and multifunction to impart the scaffolds with improved applicability is still a challenge. Here, we propose a novel fish-derived scaffold combined with photothermal therapy and mesenchymal stem cells (MSCs) to promote bone regeneration. The fish-derived scaffold is composed of the decellularized fish scale and gelatin methacrylate synthesized from fish gelatin (fGelMA), which can promote the proliferation and osteogenesis of MSCs with no obvious immunological rejection. Furthermore, the black phosphorus (BP) nanosheets are incorporated into the fGelMA hydrogel network, which can endow the hydrogel with the capacity of photothermal conversion stimulated by near-infrared (NIR) light. The fish-derived scaffold can promote the osteogenesis process of MSCs with higher expression of osteogenic markers and higher mineralization assisted by the NIR light in vitro. The regeneration of mice calvarial defect has also been accelerated by the scaffold with photothermal therapy and MSCs. These results suggest that the fish-derived scaffold, photothermal therapy, and MSCs-based regenerative therapy is a promising clinical strategy in bone regeneration.
This work was supported by the National Key Research and Development Project (No. 2021YFA1201404), Key Program of National Natural Science Foundation of China (No. 81730067), Major Project of National Natural Science Foundation of China (No. 81991514), the National Natural Science Foundation of China (No. 82101184), Shenzhen Fundamental Research Program (No. JCYJ20210324102809024), Shenzhen PhD Start-up Program (No. RCBS20210609103713045), Jiangsu Provincial Key Medical Center Foundation, Jiangsu Provincial Medical Outstanding Talent Foundation, Jiangsu Provincial Medical Youth Talent Foundation, Jiangsu Provincial Key Medical Talent Foundation, and the Fundamental Research Funds for the Central Universities (Nos. 14380493 and 14380494).