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Research Article

N, P, or S-doped carbon nanotubes as dual mimics of NADH oxidase and cytochrome c reductase

Hao Wang1,2Jinxing Chen1Qing Dong1Xu Sun1,2Qiong Liu1Dan Li1( )Erkang Wang1,2Jin Wang3( )
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
College of Chemistry, Jilin University, Changchun 130012, China
Department of Chemistry and Physics, State University of New York at Stony Brook, NY 11794-3400, USA
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Graphical Abstract

Nicotinamide adenine dinucleotide (NADH) oxidase and cytochrome c (Cyt c) reductase-like activities of carbon nanotubes (CNTs) before and after N, P, or S doping are reported, and the N-CNT showed the highest NADH oxidation activity. At the same time, the CNTs can mimic the properties of Cyt c reductase by promoting the transfer of electrons from NADH to Cyt c.

Abstract

Most nanozyme research is limited to oxidase and peroxidase. Here, we reported the N, P, or S doped carbon nanotubes (CNTs) for enzyme mimics of nicotinamide adenine dinucleotide (NADH) oxidase and cytochrome c (Cyt c) reductase. Through the doping of N element, the NADH oxidase-like activity of CNTs is highly improved, and the maximum initial velocity for N doped CNT (N-CNT) is increased by 4.28 times compared to that before the modification. Through the analysis of NADH oxidation products, we found that biologically active NAD+ was produced, and the oxygen was selectively reduced to water or hydrogen peroxide, which is consistent with natural NADH oxidase. Furthermore, we found for the first time that carbon nanotubes can promote the transfer of electrons from NADH to Cyt c, thereby can mimic the properties of Cyt c reductase.

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Nano Research
Pages 6615-6621
Cite this article:
Wang H, Chen J, Dong Q, et al. N, P, or S-doped carbon nanotubes as dual mimics of NADH oxidase and cytochrome c reductase. Nano Research, 2023, 16(5): 6615-6621. https://doi.org/10.1007/s12274-023-5393-8
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Received: 04 October 2022
Revised: 06 December 2022
Accepted: 07 December 2022
Published: 04 February 2023
© Tsinghua University Press 2023
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