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Nano Research 2023, 16(5): 6615-6621 https://doi.org/10.1007/s12274-023-5393-8
Research Article | Issue | Published: 04 February 2023

N, P, or S-doped carbon nanotubes as dual mimics of NADH oxidase and cytochrome c reductase

Show Author's Information Hao Wang1,2,  Jinxing Chen1,  Qing Dong1,  Xu Sun1,2,  Qiong Liu1,  Dan Li1( ),  Erkang Wang1,2,  Jin Wang3( )
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
College of Chemistry, Jilin University, Changchun 130012, China
Department of Chemistry and Physics, State University of New York at Stony Brook, NY 11794-3400, USA
Keywords:
carbon nanotubes, enzyme mimic, nicotinamide adenine dinucleotide (NADH) oxidase, cytochrome c reductase
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Wang H, Chen J, Dong Q, et al. N, P, or S-doped carbon nanotubes as dual mimics of NADH oxidase and cytochrome c reductase. Nano Research, 2023, 16(5): 6615-6621. https://doi.org/10.1007/s12274-023-5393-8
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Abstract Full text Electronic supplementary material About this article

Most nanozyme research is limited to oxidase and peroxidase. Here, we reported the N, P, or S doped carbon nanotubes (CNTs) for enzyme mimics of nicotinamide adenine dinucleotide (NADH) oxidase and cytochrome c (Cyt c) reductase. Through the doping of N element, the NADH oxidase-like activity of CNTs is highly improved, and the maximum initial velocity for N doped CNT (N-CNT) is increased by 4.28 times compared to that before the modification. Through the analysis of NADH oxidation products, we found that biologically active NAD+ was produced, and the oxygen was selectively reduced to water or hydrogen peroxide, which is consistent with natural NADH oxidase. Furthermore, we found for the first time that carbon nanotubes can promote the transfer of electrons from NADH to Cyt c, thereby can mimic the properties of Cyt c reductase.


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About this article

N, P, or S-doped carbon nanotubes as dual mimics of NADH oxidase and cytochrome c reductase

Show Author's information Hao Wang1,2, Jinxing Chen1, Qing Dong1, Xu Sun1,2, Qiong Liu1, Dan Li1( ), Erkang Wang1,2, Jin Wang3( )
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
College of Chemistry, Jilin University, Changchun 130012, China
Department of Chemistry and Physics, State University of New York at Stony Brook, NY 11794-3400, USA

Abstract

Most nanozyme research is limited to oxidase and peroxidase. Here, we reported the N, P, or S doped carbon nanotubes (CNTs) for enzyme mimics of nicotinamide adenine dinucleotide (NADH) oxidase and cytochrome c (Cyt c) reductase. Through the doping of N element, the NADH oxidase-like activity of CNTs is highly improved, and the maximum initial velocity for N doped CNT (N-CNT) is increased by 4.28 times compared to that before the modification. Through the analysis of NADH oxidation products, we found that biologically active NAD+ was produced, and the oxygen was selectively reduced to water or hydrogen peroxide, which is consistent with natural NADH oxidase. Furthermore, we found for the first time that carbon nanotubes can promote the transfer of electrons from NADH to Cyt c, thereby can mimic the properties of Cyt c reductase.

Keywords: carbon nanotubes, enzyme mimic, nicotinamide adenine dinucleotide (NADH) oxidase, cytochrome c reductase

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Publication history
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Acknowledgements

Publication history

Received: 04 October 2022
Revised: 06 December 2022
Accepted: 07 December 2022
Published: 04 February 2023
Issue date: May 2023

Copyright

© Tsinghua University Press 2023

Acknowledgements

Acknowledgements

H. W., J. X. C., Q. D., X. S., Q. L., D. L., and E. K. W. thank the support from the National Key Research and Development Program of China (No. 2019YFA0709202), Natural Science Foundation of Jilin Province (No. 20220101055JC), the International Cooperation Project of Jilin Scientific and Technological Development Program (No. 20190701059GH), and the National Natural Science Foundation of China (No. 31301177).

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