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Nano Research 2023, 16(5): 7269-7279 https://doi.org/10.1007/s12274-023-5376-1
Research Article | Issue | Published: 14 February 2023

Ectopic mineralization-inspired cell membrane-based matrix vesicle analogs for in-depth remineralization of dentinal tubules for treating dentin hypersensitivity

Show Author's Information Mingjing Li1,  Xiaoran Zheng1,  Zhiyun Dong1,  Yuyue Zhang1,  Wei Wu2,  Xingyu Chen3,  Chunmei Ding1,  Jiaojiao Yang4,  Jun Luo1( ),  Jianshu Li1,4,5( )
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China
Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China
College of Medicine, Southwest Jiaotong University, Chengdu 610003, China
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology and Med-X Center for Material, Sichuan University, Chengdu 610041, China
Med-X Center for Material, Sichuan University, Chengdu 610041, China
Keywords:
bioinspiration, dentin remineralization, cell membrane coating nanotechnology, ectopic matrix vesicles, mineral precursors
Topics:
Biomaterials Cell membranes
Cite this article:
Li M, Zheng X, Dong Z, et al. Ectopic mineralization-inspired cell membrane-based matrix vesicle analogs for in-depth remineralization of dentinal tubules for treating dentin hypersensitivity. Nano Research, 2023, 16(5): 7269-7279. https://doi.org/10.1007/s12274-023-5376-1
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Abstract Full text Electronic supplementary material About this article

The invasion of etched dentinal tubules (DTs) by external substances induces dentin hypersensitivity (DH). The deep and compact occlusion of DTs is highly desirable for treating DH but still challenging due to the limited penetrability and mineralization capacities of most current desensitizers. Matrix vesicles (MVs) participate in the regulation of ectopic mineralization. Herein, ectopic MV analogs are prepared by employing natural cell membranes to endow mineral precursors with natural biointerfaces and integrated biofunctions for stimulating dentin remineralization. The analogs quickly access DTs (> 20 μm) in only 5 min and further penetrate deep into the interior of DTs (an extraordinary ~ 200 μm) in 7 days. Both in vitro and in vivo studies confirm that the DTs are efficiently sealed by the newly formed minerals (> 50 μm) with excellent resistance to wear and acid erosion, which is significantly deeper than most reported values. After repair, the microhardness of the damaged dentin can be recovered to those of healthy dentin. For the first time, cell membrane coating nanotechnology is used as a facile and efficient therapy for in-depth remineralization of DTs in treating DH with thorough and long-term effects, which provides insights into their potential for hard tissue repair.


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About this article

Ectopic mineralization-inspired cell membrane-based matrix vesicle analogs for in-depth remineralization of dentinal tubules for treating dentin hypersensitivity

Show Author's information Mingjing Li1, Xiaoran Zheng1, Zhiyun Dong1, Yuyue Zhang1, Wei Wu2, Xingyu Chen3, Chunmei Ding1, Jiaojiao Yang4, Jun Luo1( ), Jianshu Li1,4,5( )
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China
Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China
College of Medicine, Southwest Jiaotong University, Chengdu 610003, China
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology and Med-X Center for Material, Sichuan University, Chengdu 610041, China
Med-X Center for Material, Sichuan University, Chengdu 610041, China

Abstract

The invasion of etched dentinal tubules (DTs) by external substances induces dentin hypersensitivity (DH). The deep and compact occlusion of DTs is highly desirable for treating DH but still challenging due to the limited penetrability and mineralization capacities of most current desensitizers. Matrix vesicles (MVs) participate in the regulation of ectopic mineralization. Herein, ectopic MV analogs are prepared by employing natural cell membranes to endow mineral precursors with natural biointerfaces and integrated biofunctions for stimulating dentin remineralization. The analogs quickly access DTs (> 20 μm) in only 5 min and further penetrate deep into the interior of DTs (an extraordinary ~ 200 μm) in 7 days. Both in vitro and in vivo studies confirm that the DTs are efficiently sealed by the newly formed minerals (> 50 μm) with excellent resistance to wear and acid erosion, which is significantly deeper than most reported values. After repair, the microhardness of the damaged dentin can be recovered to those of healthy dentin. For the first time, cell membrane coating nanotechnology is used as a facile and efficient therapy for in-depth remineralization of DTs in treating DH with thorough and long-term effects, which provides insights into their potential for hard tissue repair.

Keywords: bioinspiration, dentin remineralization, cell membrane coating nanotechnology, ectopic matrix vesicles, mineral precursors

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Publication history
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Acknowledgements

Publication history

Received: 23 August 2022
Revised: 28 November 2022
Accepted: 03 December 2022
Published: 14 February 2023
Issue date: May 2023

Copyright

© Tsinghua University Press 2023

Acknowledgements

Acknowledgements

We are grateful for the financial support of the National Natural Science Foundation of China (Nos. 51925304, 51903175, and 51973133).

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